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Biochemical changes of serum Glutathione and Malondialdehyde by Paraqu at and Olive Oil on Adult Male Reproductive system of albino Rats

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Research Paper 01/10/2018
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Biochemical changes of serum Glutathione and Malondialdehyde by Paraqu at and Olive Oil on Adult Male Reproductive system of albino Rats

Dhuha A. Hussien, Zainab Jamal Mohammed Jawad
Int. J. Biosci.13( 4), 55-60, October 2018.
Certificate: IJB 2018 [Generate Certificate]
Key: MDAOlive oilParaquatRatsSerum Glutathione

Abstract

Paraquat (PQ) is a commonly used herbicide that induces oxidative stress via reactive oxygen species (ROS) generation. This study aimed to investigate the effects of the antioxidant (olive oil ) against PQ induced oxidative stress in rats. A total of forty eight (48)  male rats were randomly divided into 4 groups which administrated orally and daily for six weeks as follows: distilled water(C), 3mg PQ & 2ml Olive oil Kg B.wt(T1), 3mg /Kg B.wt  PQ(T2) and 2ml/Kg B.wt Olive oil (T3)respectively. Blood samples were collected after 2, 4 and 6 weeks for estimation of the biochemical parameters (serum glutathione and MDA).The trends of glutathione were significantly different (P < 0.05) across the periods in all groups. In the T1, T2groups showed a significant decreasing at 6 week. On the other hand, the T3 showed a significant increasing (P < 0.05) within 4 and 6 weeks. The results showed the means of Glutathione were significantly lowered in T1 and T2 as compared with control. While the MDA shows a significant increase (P < 0.05)in theT1 and T2during the experiment periods as compared with control group, while group (T3) treated with olive oil only shows a significant decrease (P <0.05) in MDA concentration comparing with control group. In conclusion, MDA increased in rats treated with Paraquat more than other groups treated with olive oil as antioxidant, while the glutathione is decrease at same groups and increased at groups of olive oil.

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Bindu M, Guha I, Mallika T, Behera P, Chakrabarti S. 2011“Age-related oxidative decline of mitochondrial functions in rat brain is prevented by long term oral antioxidant supplementation.” Bio gerontology 12, 119-131.

Castello P, Drechsel D, Patel M. 2007 “Mitochondria are a major source of Paraquat-induced reactive oxygen species in the brain.” The journal of biological chemistry 282, 14186-14193.

Chen CW, Wu, YH, Chien SC, M Lin JC. 2018. A  survival case of intravenous paraquat intoxication: A case report. Medicine 97( 30), 11669-11684.

Chen Q, Niu Y, Zhang R, Guo H, Gao Y, Li Y, 2010. “The toxic influence of paraquat on hippocampus of mice: involvement of oxidative stress.” Neurotoxic ology 31, 310-316.

Chen Q, Xin Z, Jin0Yan Z, Xiao-Ning L, Peng-Sheng ZH, Xiang X. 2017. Oxidative damage of the male reproductive system induced by paraquat. Journal of Biochemical and Molecular Toxicology 31(3). http://dx.doi.org/10.1002/jbt.21870

Couto N, Malys N, Gaskell SJ, Barber J. 2013. Partition and turnover of glutathione reductase from Saccharomyces cerevisiae: a proteomic approach. Journal of Proteome Research 12(6), 2885–94.

Devasagayam T, Tilak JC, Boloor KK, Sane Ketaki S, Ghaskadbi Saroj S, Lele RD. 2004. “Free Radicals and Antioxidants in Human Health: Current Status and Future Prospects.” Journal of Association of Physicians of India (JAPI) 52, p 796-797.

Gheita TA, Kenawy SA. 2014. Measurement of malondialdehyde, glutathione, and glutathione peroxidase in SLE patients. Methodsin Molecular Biology 1134, 193-9. http://dx.doi.org/10.1007/978-1-4939-0326-9_14.

Hafez  E. 1970. Reproduction and Breeding Techniques for Laboratory Animals. Lea and Fibiger, Philadelphia:, London.

Harman D. 1956. Aging: a theory based on free radical and radiation chemistry. Journal of Gerontology 11, 298-300.

Lykkesfeldt J. 2007. Malondialdehyde as biomarker of oxidative damage to lipids caused by smoking. Clinical Chimes try  Acta. 380, 50–58.

Meister A. 1991. Glutathione deficiency produced by inhibition of its synthesis, and its reversal: applications in research and therapy. Pharmacology and Therapeutics Journal 51, 155-194.

Mirhoseini M, Mohamadpour M, Khorsandi L.2012. Toxic effects of Carthamus tinctorius L. (Safflower) extract on mouse spermatogenesis. Journal of Assisted Reproduction and Genetics 29, 457–461.

Nair V, Crystal L, O’Neil PGW.2008. Malondialdehyde”, Encyclopedia of Reagents for Organic Synthesis. John Wiley & Sons, New York.

Nguyen NV, Nguyen NTT, Thao NTB, Phuoc TH. 2015. The effect of paraquat herbicide on in vivo spermatogenesis in mice. Journal of Science Ho Chi Minh City Open University 1(13), 3-7.

Oja S, Janáky R, Varga V, Saransaari P. 2000. Modulation of glutamate receptor functions by glutathione. Neurochemistry International 37(2–3), 299–306.

Pompella A, Visvikis A, Paolicchi A, De Tata V, Casini AF. 2003. The changing faces of glutathione, a cellular protagonist. Biochemical Pharmacology 66(8), 1499–503.

SAS. 2010. SAS/STAT Users Guide for Personal Computer. Release 9.13.SAS Institute, Inc., Cary, N.C., USA.

Serafani Mauro, Del Rio Daniele. 2004 “Understanding the association between dietary antioxidants, redox status and disease: is the total antioxidant capacity is the right tool?” Redox Report, 9, 145-152.

Servili M, Esposto S, Fabiani R, Urbani S, Taticchi A, Mariucci F, Selvaggini R, Montedoro GF. 2009. Phenolic compounds in olive oil: antioxidant, health and organoleptic activities according to their chemical structure  Inflammopharmacology 17(2), 76-84.

Yamamoto H. 1993. Protection against paraquat induced toxicity with sulfite or thiosulfate in mice. Toxicology 79, 37-43.

Yonny ME, García EM, López A, JoséI A, Nazareno MA. 2016. Measurement of malondialdehyde as oxidative stress biomarker in goat plasma by HPLC-DAD. Micro chemical Journal 129, 281-28.

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