Biodiversity of cultivated Trigonella foenum-graecum L. in Tunisia, North Africa

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Research Paper 01/04/2015
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Biodiversity of cultivated Trigonella foenum-graecum L. in Tunisia, North Africa

Nidhal Marzougui, Hédia Hannachi, Anissa Boubaya, Ali Ferchichi, Salwa Rejeb
J. Bio. Env. Sci.6( 4), 138-151, April 2015.
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In agricultural systems, biodiversity includes diversity within species and among species and provides many benefits for production, resilience and conservation. This article aims to study the diversity of Trigonella foenum-graecum L., an annual herb of Leguminosae with a worldwide distributed culture, among thirty eight local populations collected from different Tunisian regions. The variability analysis was based on morphological characters, compositions of minerals in leaves and vitamins in seeds, and molecular profile. Morphological parameters consisted of vegetative and reproductive characters. Mineral analysis concerned sodium, potassium, iron, calcium and magnesium leaves contents. The analyzed vitamins contents in seeds were B1, B9 and C vitamins and molecular study was carried out by ISSR technique. The structure of the studied populations was established by the principal compound analysis (PCA) and by the unweighted pair group method using arithmetic means (UPGMA). Both analyses based on the combination of morphological, chemical and biochemical parameters and on ISSR molecular study, presented a binary clustering of populations; but the one was different from the other. The combination of morphological, chemical and biochemical parameters allowed distinguishing the populations 2 and 4 of Menzel Temime, 11 of Mateur and 13 of Beja from the remaining populations. These four populations had the highest yields in biomass and seeds and, compared to the rest of populations, they were the best forages and seed producers. Molecular study showed that population 26 of Menzel Temime was genetically far away from the rest of studied fenugreek populations.


Acharya SN, Blade S, Mir Z, Moyer JR. 2007. Tristar fenugreek. Canadian Journal of Plant Science 87, 901-903.

Arella F, Deborde JL, Bourguignon JB, Hasselmann C. 1997. High performance liquid chromatographic determination of L-ascorbic acid and total vitamin C in foodstuffs: Interlaboratory study. Annales des Falsifications et de l’Expertise Chimique et Toxicologique 90, 217-233.

Basha SD, Francis G, Makkar HPS, Becker K, Sujatha M. 2009. A comparative study of biochemical traits and molecular markers for assessment of genetic relationships between Jatropha curcas L. germplasm from different countries. Plant Science 176, 812-823.

Beyene Y, Botha AM, Myburg AA. 2005. A comparative study of molecular and morphological methods of describing genetic relationships in traditional Ethiopian highland maize. African Journal of Biotechnology 4, 586-595.

Bruschi P, Vendramin GG, Bussotti F, Grossoni P. 2003. Morphological and molecular diversity among Italian populations of Quercus petraea. Annals of Botany 91, 7-16.

Chahidi B, El-Otmani M, Jacquemond C, Tijane M, El-Mousadik A, Srairi I, Luro F. 2008. Utilisation de caractères morphologiques, physiologiques et de marqueurs moléculaires pour l’évaluation de la diversité génétique de trois cultivars de clémentinier. Comptes rendus Biologies 331, 1-12.

Collmer K, Davies L. 1974. Separation and determination of vitamins B1, B2, B6 and nicotinamid in commercial vitamins preparation using high performance cation exchange chromatography. Chromatography 11, 635-644.

Dey SS, Singh AK, Chandel D, Behera TK. 2006. Genetic diversity of bitter gourd (Momordica charantia L.) genotypes revealed by RAPD markers and agronomic traits. Scientia Horticulturae 109, 21-28.

Duke AJ. 1986. Handbook of legumes of world economic importance. 1st Ed. Plenum Press. NewYork, USA.

Doyle JJ, Doyle JL. 1987. A rapid DNA isolation procedure for small amount of fresh leaf tissue. Phytochemical Bulletin 19, 11-15.

Enjalbert J, Dawson JC, Paillard S, Rhoné B, Rousselle Y, Thomas M, Goldringer I. 2011. Dynamic management of crop diversity: From an experimental approach to on-farm conservation. Comptes Rendus Biologies 334, 458-68.

Espósito MA, Martin EA, Cravero VP, Cointry E. 2007. Characterization of pea accessions by SRAP’s markers. Scientia Horticulturae 4, 329-335.

Kyndt T, Romeijn-Peeters E, Van Droogenbroeck B, Romero-Motochi JP, Gheysen G, Goetghebeur P. 2005. Species relationships in the genus Vasconcellea (Caricaceae) based on molecular and morphological evidence. American Journal of Botany 92, 1033-1044.

Malik SS, Singh SP. 2006. Role of plant genetic resources in sustainable agriculture. Indian Journal of Crop Science 1, 21-28.

Mantel N. 1967. Detection of disease grouping and a generalized regression approach. Cancer Research 27, 209-220.

Marzougui N. 2010. Diversité génétique et amélioration par polyploïdie artificielle de populations tunisiennes de Trigonella foenum-graecum L. PhD thesis, Higher Institute of Biotechnology of Monastir, Tunisia.

Marzougui N, Boubaya A, Elfalleh W, Guasmi F, Laaraiedh L, Ferchichi A Triki T, Beji M. 2009. Assessment of genetic diversity in Trigonella foenum-graecum Tunisian cultivars using ISSR markers. Journal of Food Agriculture and Environment 5, 245-250.

Marzougui N, Ferchichi A, Guasmi F, Beji M. 2007. Morphological and chemical diversity among 38 Tunisian cultivars of Trigonella foenum-graecum L. Journal of Food Agriculture and Environment 5, 245-250.

Marzougui N, Guasmi F, Mkaddem M, Boubaya A, Mrabet A, Elfalleh W, Ferchichi A, Beji M. 2009. Assessment of Tunisian Trigonella foenum-graecum diversity using seed vitamin B6, B1, B9 and C contents. Journal of Food Agriculture and Environment 5,

Mkaddem M. 2007. Mentha Pelegium L. en Tunisie: diversité génétique et variabilité de la composition des huiles essentielles des populations naturelles. PhD thesis. Faculty of sciences of Tunis. Tunis, Tunisia.

Nei M. 1978. Estimation of average heterozygosis and genetic distance from a small number of individuals. Genetics 89, 583-590.

Nei M, Li WH. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Genetics 76, 5269-5273.

Petropoulos AG. 2002. Fenugreek. The genus Trigonella. Georgios A. Petropoulos Ed. Taylor & Francis. London, New York.

Ramesh HP, Yamaki K, Ono H, Tsushida T. 2001. Two-dimensional NMR spectroscopic studies of fenugreek (Trigonella foenum-graecum L.) galactomannan without chemical fragmentation. Carbohydrate Polymers 45, 69-77.

Smith JSC, Smith OS. 1992. Fingerprinting crop varieties. Advances in agronomy 47, 85-140.

Statti GA, Conforti F, Sacchetti G, Muzzoli M, Agrimonti C, Menichini F. 2004. Chemical and biological diversity of Bergamot (Citrus bergamia) in relation to factors environmental. Fitoterapia 75, 212–216.

Tar’an  B,  Zhang  C,  Warkentin  T,  Tullu  A, Vandenderg A. 2005. Genetic diversity among varieties and wild species accessions of pea (Pisum sativum) based on molecular markers and morphological and physiological characters. Genome 48, 57-72.

Toma RB, Tabekhia M. 1979. High performance liquid chromatographic analysis of vitamins in rice and rice products. USA Journal of Food Science 44, 263-268.

Tucak M, Popović S, Čupić T, Grljušić S, Bolarić S, Kozumplik V. 2008. Genetic diversity of alfalfa (Medicago spp.) estimated by molecular markers and morphological characters. Periodicum Biologorum 110, 243-249.

Vats V, Grover JK, Rathi SS. 2002. Evaluation of anti- hyperglycemic and hypoglycaemic effect of Trigonella foenum-graecum, Ocimum sanctum and Pterocarpus marsupium Linn in normal and allxanized diabetic rats. Journal of Ethnopharmacology 79, 95-100.

Vigouroux Y, Barnaud A, Scarcelli N, Thuillet AC. 2011. Biodiversity, evolution and adaptation of cultivated crops. Comptes Rendus Biologies 334, 450-7.

Vinas P, Lopez Erroz C, Balsalobre N, Hernandez Cordoba M. 2003. Reversed-phase liquid chromatography on an amide stationary phase for the determination of the B group vitamins in baby foods. Journal of Chromatography A 10, 77-84.