Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | October 1, 2015

| Download 1

Comparative analysis of some biochemical responses of winter and spring wheat cultivars under low temperature

Ahmad Tahmasebi, Hassan Pakniyat

Key Words:

Int. J. Agron. Agri. Res.7(4), 14-22, October 2015


IJAAR 2015 [Generate Certificate]


To compare changes of biochemical indices between spring (Kavir) and winter (Azar2) cultivars of wheat (Triticum aestivum L.) under low temperature, 14 days old wheat seedlings were exposed to cold. The seedlings were transferred into growth chamber for 9 days at 5/3 °C (day/night) as cold treatment, or at 20/18 °C as control. Proline content, total protein accumulation, activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) enzymes, were assayed in the leaf extracts of control and cold treated plants. The results showed that cold led to an accumulation of proline and an increase in protein level, especially in winter cultivar. Rapid increases in proline and protein accumulations were observed during early stages of cold stress. SOD activity displayed no significant differences between the two cultivars during the first 3 days after cold stress, while in Azar 2, the level of SOD activity was gradually increased after 3 days of cold stress. The POD and CAT activity were higher in plants grown at cold stress than in the controls; however, their rate was different in winter and spring wheat cultivars. In general, Azar2 showed relatively higher POD and CAT activity compared to Kavir. Regarding antioxidant enzymes activities, cultivars respond differently under cold stress.


Copyright © 2015
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Comparative analysis of some biochemical responses of winter and spring wheat cultivars under low temperature

Abedi T, Pakniyat H. 2010. Antioxidant enzyme changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.). Czech Journal of Genetics and Plant Breeding 46, 27-34.

Aebi H. 1984. Catalase in vitro. Methods Enzymol 105, 121-126.

Apel K, Hirt H. 2004. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology 55, 373-399.

Ashraf M, Foolad M. 2007. Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany 59, 206-216.

Badiani M, et al. 1997. Non-optimal growth temperatures and antioxidants in the leaves of Sorghum bicolor (L.) Moench. II. Short-term acclimation. Journal of Plant Physiology 151, 409-421.

Bates L, Waldren R, Teare I. 1973. Rapid determination of free proline for water-stress studies. Plant and Soil 39, 205-207.

Beauchamp C, Fridovich I. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 44, 276-287.

Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248-254.

Chance B, Maehly A. 1955. Assay of catalases and peroxidases. Methods in Enzymology 2, 764-775.

Dionne J, Castonguay Y, Nadeau P, Desjardins Y. 2001. Amino Acid and Protein Changes during Cold Acclimation of Green-Type Annual Bluegrass (L.) Ecotypes. Crop Science 41, 1862-1870.

Fowler DB, Breton Gn, Limin AE, Mahfoozi S, Sarhan F. 2001. Photoperiod and temperature interactions regulate low-temperature-induced gene expression in barley. Plant Physiology 127, 1676-1681.

Galiba G, Vagujfalvi A, Li C, Soltesz A, Dubcovsky J. 2009. Regulatory genes involved in the determination of frost tolerance in temperate cereals. Plant Science 176, 12-19.

Heidari M, Golpayegani A. 2012. Effects of water stress and inoculation with plant growth promoting rhizobacteria (PGPR) on antioxidant status and photosynthetic pigments in basil (Ocimum basilicum L.). Journal of the Saudi Society of Agricultural Sciences 11, 57-61.

Hoque MA, Banu MNA, Nakamura Y, Shimoishi Y, Murata Y. 2008. Proline and glycinebetaine enhance antioxidant defense and methylglyoxal detoxification systems and reduce NaCl-induced damage in cultured tobacco cells. Journal of Plant Physiology 165, 813-824.

Huang M, Guo Z. 2005. Responses of antioxidative system to chilling stress in two rice cultivars differing in sensitivity. Biologia Plantarum 49, 81-84.

Huner NP, et al. 1987. Low temperature development induces a specific decrease in trans-Δ3-hexadecenoic acid content which influences LHCII organization. Plant Physiology 84, 12-18.

Iannelli MA, Van Breusegem F, Van Montagu M, Inzé D, Massacci A. 1999. Tolerance to low temperature and paraquat-mediated oxidative stress in two maize genotypes. Journal of Experimental Botany 50, 523-532.

Kasuga M, Liu Q, Miura S, Yamaguchi-Shinozaki K, Shinozaki K. 1999. Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Nature Biotechnology 17, 287-291.

Khedr AHA, Abbas MA, Wahid AAA, Quick WP, Abogadallah GM. 2003. Proline induces the expression of salt‐stress‐responsive proteins and may improve the adaptation of Pancratium maritimum L. to salt‐stress. Journal of experimental botany 54, 2553-2562.

Krishnamurthy A, Rathinasabapathi B. 2013. Oxidative stress tolerance in plants: Novel interplay between auxin and reactive oxygen species signaling. Plant Signaling and Behavior 8, e25761.

Lang P, Zhang C-k, Ebel RC, Dane F, Dozier WA. 2005. Identification of cold acclimated genes in leaves of Citrus unshiu by mRNA differential display. Gene 359, 111-118.

Levitt J. 1980. Responses of plants to environmental stresses. Volume II. Water, radiation, salt, and other stresses. Academic Press.

Matysik J, Bhalu B, Mohanty P. 2002. Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plants. Current Science 82, 525-532.

Mohapatra SS, Poole RJ, Dhindsa RS. 1987. Cold Acclimation, Freezing Resistance and Protein Synthesis in Alfalfa (Medicago sativa L. cv. Saranac). Journal of Experimental Botany 38, 1697-1703.

Otter T, Polle A. 1994. The influence of apoplastic ascorbate on the activities of cell wall-associated peroxidase and NADH oxidase in needles of Norway spruce (Picea abies L.). Plant and Cell Physiology 35, 1231-1238.

Papageorgiou GC, Murata N. 1995. The unusually strong stabilizing effects of glycine betaine on the structure and function of the oxygen-evolving photosystem II complex. Photosynthesis Research 44, 243-252.

Patton AJ, Cunningham SM, Volenec JJ, Reicher ZJ. 2007. Differences in freeze tolerance of zoysiagrasses: II. Carbohydrate and proline accumulation. Crop Science 47, 2170-2181.

Raza SH, Athar HR, Ashraf M, Hameed A. 2007. Glycinebetaine-induced modulation of antioxidant enzymes activities and ion accumulation in two wheat cultivars differing in salt tolerance. Environmental and Experimental Botany 60, 368-376.

Repo T, Mononen K, Alvila L, Pakkanen TT, Hänninen H. 2008. Cold acclimation of pedunculate oak (Quercus robur L.) at its northernmost distribution range. Environmental and Experimental Botany 63, 59-70.

Scandalios JG. 1993. Oxygen stress and superoxide dismutases. Plant Physiology 101, 7.

Suzuki N, Mittler R. 2006. Reactive oxygen species and temperature stresses: a delicate balance between signaling and destruction. Physiologia Plantarum 126, 45-51.

Szabados L, Savoure A. 2010. Proline: a multifunctional amino acid. Trends in Plant Science 15, 89-97.

Szalai G, Pap M, Janda T. 2009. Light-induced frost tolerance differs in winter and spring wheat plants. Journal of Plant Physiology 166, 1826-1831.

Urquiaga I, Leighton F. 2000. Plant polyphenol antioxidants and oxidative stress. Biological Research 33, 55-64.

Wang X, Li W, Li M, Welti R. 2006. Profiling lipid changes in plant response to low temperatures. Physiologia Plantarum 126, 90-96.

Yeh S, et al. 2000. Chitinase genes responsive to cold encode antifreeze proteins in winter cereals. Plant Physiology 124, 1251-1264.


Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background