Comparative antibiotic resistance profile of Acinetobacter spp. isolated from fish, chicken and beef meat

Paper Details

Research Paper 01/04/2019
Views (462) Download (11)

Comparative antibiotic resistance profile of Acinetobacter spp. isolated from fish, chicken and beef meat

Aftab Ahmad, Fareeha Akhtar, Muhammad Ilyas Khan, Hameed Ur Rehman, Syeda Alia Gerdazi, Uzma Ghani, Muhammad Hanzalah Khan, Uzma Ayaz, Hira Mushtaq, Nausheen Abdul Malik, Nasir Mahmood, Matiullah, Wahid Shah, Malik Nawaz Shuja
Int. J. Biosci.14( 4), 305-315, April 2019.
Certificate: IJB 2019 [Generate Certificate]


Acinetobacter is a gram-negative bacterium which is found to be involved in serious health implications due to consumption of contaminated food. In present study different markets of Lahore were selected to collect fish, beef and chicken meat samples. Identification was performed by using microscopic, morphological, biochemical and molecular analysis of all the samples. Sample were subjected to antibiotic resistance to create a comparative profile for antibiotic resistance for fish, beef and chicken meat. In the present study the molecular identification was used for carbapenem resistance gene (SPM-1). The results indicated that 24 (26.6%) of total samples were positive for Acinetobacter species. The prevalence of the Acinetobacterspecies was (30%) (26.6%) and (23.3%) in fish meat, chicken meat and beef, respectively. Ampicillin and trimethoprim showed highest resistance 100% followed by tetracycline 100%, 75% and 66.6% in beef, chicken and fish isolates respectively. Only 17 of 24 isolates were confirmed as targeted bacterium for the SPM-1 gene by PCR. Acinetobacter species had low percentage in beef meat than in fish meat and chicken meat. The chicken meat is contaminated with antibiotics resistance Acinetobacter species, they can cause serious diseases in future in poultry, animals and human. The risk concern with antibiotics resistance Acinetobacter species cannot be ignored because, they resist to currently using potential antibiotics.


Doughari HJ, Ndakidemi PA, Human IS, Benade S. 2011. The ecology, biology and pathogenesis of Acinetobacter spp.: an overview. Microbes and environment 26, 101-112.

Berlau J, Aucken H, Houang E, Pitt T. 1999.  Isolation of Acinetobacter spp including A. baumannii from vegetables: implications for hospital-acquired infections. Journal of Hospital Infection 42,201-204.

Čož-Rakovac R, Strunjak-Perović I, Popović NT, Hacmanjek M, Šimpraga B, Teskeredžić E. 2002. Health status of wild and cultured sea bass in the northern Adriatic Sea. Veterinary Med-Czech. 4, 7, 222-226.

Wang Y, Wu C, Zhang Q, Qi J, Liu H, Wang Y, He T, Ma L, Lai J, Shen Z. 2012. Identification of New Delhi metallo-β-lactamase 1 in Acinetobacter lwoffii of food animal origin. PloS one 7, e37152.

Doughari HJ, Ndakidemi PA, Human IS, Benade S. 2011. The ecology, biology and pathogenesis of Acinetobacter spp.: an overview. Microbes and environment 26, 101-112.

Rafei R, Hamze M, Pailhoriès H, Eveillard M, Marsollier L, Joly-Guillou M-L, Dabboussi F, Kempf M. 2015.Extrahuman epidemiology of Acinetobacter baumannii in Lebanon. Applied Environmental microbiology 81, 2359-2367.

Jalil H, Hussain SS, Siddiqi AF. 2013. An empirical study of meat supply chain and prices pattern in lahore (pakistan): A case study.

Chouliara E, Badeka A, Savvaidis I, Kontominas MG. 2008. Combined effect of irradiation and modified atmosphere packaging on shelf-life extension of chicken breast meat: microbiological, chemical and sensory changes. E. Food Research Technology 226, 877-888.

Gill C, Newton K. 1978. The ecology of bacterial spoilage of fresh meat at chill temperatures. Meat Science 2, 207-217.

Abbo A. 2005. Multidrug-Resistant Acinetobacter baumannii-Volume 11, Number 1—January 2005-Emerg Infect Dis J-CDC.

Koińska, Paźdior E, Pękala, Niemczuk W. 2014. Acinetobacter johnsonii and Acinetobacterl woffii-the emerging fish pathogens. B Vet I Pulawy. 58, 193-199.

Jung J, Park W. 2015.Acinetobacter species as model microorganisms in environmental microbiology: current state and perspectives. Applied microbial Biotechnology 99, 2533-2548.

Fournier PE, Richet H, Weinstein RA. 2006. The epidemiology and control of Acinetobacter baumannii in health care facilities. Clinical Infectious Disease 42, 692-699.

Guardabassi L, Dalsgaard A, Olsen J. 1999. Phenotypic characterization and antibiotic resistance of Acinetobacter spp. isolated from aquatic sources. Journal of applied microbiology 87, 659-667.

McDonald LC. 2006. Trends in antimicrobial resistance in health care–associated pathogens and effect on treatment. Clinical Infectious Disease 42, S65-S71.

Eliopoulos GM, Cosgrove SE, Carmeli Y. 2003. The impact of antimicrobial resistance on health and economic outcomes. Clinical Infectious Disease 36, 1433-1437.

Antwi-Agyei P, Maalekuu B. 2014. Determination of microbial contamination in meat and fish products sold in the Kumasi metropolis (A Case Study of Kumasi central market and the Bantama market). MRJASSS 2, 38-46.

Cheesbrough M.  2006. District laboratory practice in tropical countries.  Part 2, 2nd ed. Cambridge University Press. The Edinburgh building, Cambridge.

Vos P, Garrity G, Jones D, Krieg NR, Ludwig W, Rainey FA, Schleifer KH, Whitman WB.2011.Bergey’s manual of systematic bacteriology: 3, TheFirmicutes3, (Springer Science & Business Media).

Lee IM, Shiroma EJ, Lobelo F, Puska P, Blair SN, Katzmarzyk PT,2012. “Effect of physical inactivity on major non-communicable diseases worldwide: an analysis of burden of disease and life expectancy. Lancet 21(380),219-29.

Moradi J, Hashemi FB, Bahador A. 2015. Antibiotic resistance of Acinetobacterbaumannii in Iran: a systemic review of the published literature. Osong Public Health Res Perspect. 6, 79-86.

Kulah C, Aktas E, Comert F, Ozlu N, Akyar I, Ankarali H. 2009. Detecting imipenem resistance in Acinetobacter baumannii by automated systems (BD Phoenix, MicroscanWalkAway, Vitek 2); high error rates with MicroscanWalkAway. BMC infectious Diseases 9, 30.

Phillips I, Casewell M, Cox T, De Groot B, Friis C, Jones R, Nightingale C, Preston R, Waddell J. 2004. Does the use of antibiotics in food animals pose a risk to human health? A critical review of published data. Journal of Antimicrobial Chemotherapy 53, 28-52.