Int. J. Biosci.11( 4), 312-319, October 2017
The deleterious effects of oxidative stress caused by accumulation of free radicals in human body has gathered tremendous attention in the recent years. Wild medicinal mushrooms are rich in secondary metabolites that make them effective against a wide range of diseases. The present study was conducted to determine the antioxidant activity of two wild mushroom species; Phellinus baumii and Trametes versicolor from Pakistan. The 80% methanolic extract of each mushroom species was prepared and tested by using in vitro antioxidant assays such as 2, 2-diphenyl-1-picrylhydrazy (DPPH) and hydrogen peroxide (H2O2) scavenging assays. The results revealed that P. baumii possessed remarkable antioxidant activity against free radicals by showing (83.4±0.1%) and (81.9 %±1.0%) scavenging effects on DPPH and H2O2 radicals as compared to T. versicolor. In addition, the total phenolic content was measured by Folin-Ciocalteau assay, indicating the presence of high phenolic content in P. baumii (27.9±2.68 mg GAE/g dw) as compared to 6.18±2.2mg GAE/g dw for T. versicolor. These findings suggested that P. baumii could be used as a source of natural antioxidants in pharmaceutical industry.
Barja G. 2004. Free radicals and aging. Trends in Neurosciences 27(10), 595-600. https://doi.org/ 10.1016/j.tins.2004.07.005
Chun-hui L, Chang-hai W, Zhi-liang X, Yi W. 2007. Isolation, chemical characterization and antioxidant activities of two polysaccharides from the gel and the skin of Aloe barbadensis Miller irrigated with sea water. Process Biochemistry 42(6), 961-970. https://doi.org/10.1016/j.procbio.2007.03.004
Ferreira ICFR, Baptista P, Vilas-Boas M, Barros L. 2007. Free-radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: Individual cap and stipe activity. Food chemistry 100(4), 1511-1516. https://doi. org/10.1016/j.foodchem.2005.11.043
Ferreira ICFR, Barros L, Abreu R. 2009. Antioxidants in wild mushrooms. Current Medicinal Chemistry 16(12), 1543-1560. https://doi.org/10. 2174/092986709787909587
Finkel T, Holbrook NJ. 2000. Oxidants, oxidative stress and the biology of ageing. Nature 408(6809), 239-247. https://doi.org/10.1038/35041687
Kamiyama M, Horiuchi M, Uman K, Kondo K, Otsuka Y, Shibamoto T. 2013. Antioxidant/anti-inflammatory activities and chemical composition of extracts from the mushroom Trametes versicolor. International Journal of Food Sciences and Nutrition 2, 85-91. https://doi.org/10.11648/j.ijnfs.20130202.19
Kim MY, Seguin P, Ahn JK, Kim JJ, Chun SC, Kim EH, Seo SH, Kang EY, Kim SL, Park YJ, Ro HM, Chung IM. 2008. Phenolic compound concentration and antioxidant activities of edible and medicinal mushrooms from Korea. Journal of Agricultural and Food Chemistry 56(16), 7265-7270. https://doi.org/10.1021/jf8008553
Lachance PA, Nakat Z, Jeong WS. 2001. Antioxidants: an integrative approach. Journal of Nutrition 17, 835-838. http://dx.doi.org/10.1016/ S0899-9007(01)00636-0
Li HY, Hao ZB, Wang XL, Huang L, Li JP. 2009. Antioxidant activities of extracts and fractions from Lysimachia foenum-graecum Hance. Bioresource Technology 100, 970-974. https://doi. org/10.1016/j.biortech.2008.07.021
Liu J, Jia L, Kan J, Jin CH. 2013. In vitro and in vivo antioxidant activity of ethanolic extract of white button mushroom (Agaricus bisporus). Food and Chemical Toxicology 51, 310-316. https://doi.org/10.1016/j.fct.2012.10.014
Liu L, Sun Y, Laura T, Liang X, Ye H, Zeng X. 2009. Determination of polyphenolic content and antioxidant activity of kudingcha made from Ilex kudingcha CJ Tseng.. Food chemistry 112(1), 35-41. https://doi.org/10.1016/j.foodchem.2008.05.038
Mates JM, Pérez-Gómez C, Castro DANI. 1999. Antioxidant enzymes and human diseases. Clinical Chemistry 32(8), 595-603. https://doi.org/10.1016/ S0009-9120(99)00075-2
Mau JL, Lin HC, Chen CC. 2002. Antioxidant properties of several medicinal mushrooms. Journal of Agricultural and Food Chemistry 50(21), 6072-6077. https://doi.org/10.1021/jf0201273
Mc Call MR, Frei B. 1999. Can antioxidant vitamins materially reduce oxidative damage in humans?. Free Radical Biology and Medicine 26(7-8), 1034-1053. https://doi.org/10.1016/S0891-5849(98)00302-5
Michael HW, Bultosa G, Pant LM. 2011. Nutritional contents of three edible oyster mushrooms grown on two substrates at Haramaya, Ethiopia, and sensory properties of boiled mushroom and mushroom sauce. International Journal of Food Science and Technology 46(4), 732-738. https://doi.org/10.1111/j.1365-2621.2010.02543.x
Perry G, Moreira PI, Santos MS, Oliveira CR, Shenk JC, Nunomura A, Smith MA, Zhu X. 2008. Alzheimer disease and the role of free radicals in the pathogenesis of the disease. CNS and Neurological Disorders Drug Targets 7(1), 3-10. https://doi.org/10.2174/187152708783885156
Ridnour LA, Isenberg JS, Espey MG, Thomas DD, Roberts DD, Wink DA. 2005. Nitric oxide regulates angiogenesis through a functional switch involving thrombospondin-1. Proceedings of the National Academy of Sciences of USA. 102(37),13147-13152. https://doi.org/10.1073/pnas.0502979102
Ruch RJ, Cheng SJ, Klaunig JE. 1989. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Journal of Carcinogenesis 10(6), 1003-1008. https://doi.org/10.1093/carcin/10.6.1003
Ruch RJ, Chug SU, Klaunig JE. 1984. Spin Trapping of Superoxide and Hydroxyl Radicals. Method in Enzymology 105, 198-209. https://doi. org/0.1016/S0076-6879(84)05026-6
Shah AM, Channon KM. 2004. Free radicals and redox signalling in cardiovascular disease. Heart Journal 90, 486-487. http://dx.doi.org/10.1136/ hrt.2003.029389
Shanlin FU, Davies, MJ, Stocker R. 1998. Evidence for roles of radicals in protein oxidation in advanced human atherosclerotic plaque. Biochemical Journal 333(3), 519-525. https://doi. org/10.1042/bj3330519
Sheikh IA, Vyas DEEPAK, Ganaie MOHDANIS, Dehariya K, Singh VINITA. 2014. HPLC determination of phenolics and free radical scavenging activity of ethanolic extracts of two polypore mushrooms. International Journal of Pharmacy and Pharmaceutical Sciences 6(1), 679-684.
Shimada K, Fujikawa K, Yahara K, Nakamura T. 1992. Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. Journal of Agricultural and Food Chemistry 40(6), 945-948. https://doi.org/10.1021/jf00018a005
Shon MY, Kim TH, Sung NJ. 2003. Antioxidants and free radical scavenging activity of Phellinus baumii (Phellinus of Hymenochaetaceae) extracts. Food chemistry 82(4), 593-597. https://doi.org/ 10.1016/S0308-8146(03)00015-3
Song L, Sun PI, Guo BB, Wei HH, Chen LJ. 2005. Recent studies on Sang Hunag. Edible Fungi of China 24(3), 7.
Sultana B, Anwar F, Przybylski R. 2007. Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam. trees. Food chemistry 104(3), 1106-1114. https://doi.org/10.1016/j.foodchem.2007.01.019
Valko M, Rhodes C, Moncol J, Izakovic MM, Mazur M. 2006. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemico-Biological Interactions 160(1), 1-40. https://doi.org/10.1016/j.cbi.2005.12.009
Wang Y, Shang XY, Wang SJ, Mo SY, Li S, Yang YC, Ye F, Shi JG, He L. 2007. Structures, biogenesis, and biological activities of pyrano [4, 3-c] isochromen-4-one derivatives from the fungus Phellinus igniarius. Journal of Natatural Products 70(2), 296-299. https://doi.org/10.1021/np060476h
Ying CC. 1987. Icons of medicinal fungi from China. Science Press 277.
Yuting C, Rongliang Z, Zhongjian J, Yong J. 1990. Flavonoids as superoxide scavengers and antioxidants. Free Radical Biology and Medicine 9(1), 19-21. https://doi.org/10.1016/0891-5849(90)90045-K
Zeng NK, Wang QY, Su MS. 2008. Discussion on the mushrooms used for traditional Chinese medicine “Sanghuang”. Edible Fungi of China 27(2), 56-59.