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Comparison of some physicochemical properties of oil extracted from Ricinodendron heudelotii (Bail.) Kernels by UV spectrophotometry

Horliane Ghomdim Nzali, Clergé Tchiegang, Bernard Sandjon, Marc Meurens

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Int. J. Biosci.8(6), 93-102, June 2016

DOI: http://dx.doi.org/10.12692/ijb/8.6.93-102


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In order to contribute to research on nutrients with therapeutic value, the oil rate, fatty acid composition and the physicochemical and quality characteristics of Riconodencron heudelotii kernels oil obtained by pressing and solvent extraction were determined. The KOMET Screw Oil Expeller was used. To achieve this, UV Spectrophotometry and Gas Chromatography (GC) were used to quantify the amount of α-eleostearic acid (ESA) in the oils. The results indicate that the extraction temperature has an influence on the rate of extraction. Thus the extraction rate for cold pressing (30°C) was 34.51±1.98% compared to hot pressing (80°C) with 44.44±2.46%. But for the quality of the oil, cold pressing gives the indices of peroxide and acid below standard. There is no significant difference (p <0.05) of α-tocopherol according to extraction mode. The proportion of ESA of oil obtained by cold pressing (30°C) was the highest (49.02±0.64%). The UV confirmed that the press had no influence on the structure of the ESA. The calibration test for the determination of ESA by UV spectrophotometry was obtained with a Regression coefficient (R) of 0.95. The UV spectrophotometry technique is a rapid and less expensive method for determining the quality and the quantity of ESA in R. heudelotii oil than GC. The cold pressing almonds of R. heudelotii gives an oil with good physicochemical properties and is rich of α-eleostearic acid. Moreover eleostearic acid is a conjugated linolenic acid and these acids have benefic effects for the management of metabolic diseases.


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Comparison of some physicochemical properties of oil extracted from Ricinodendron heudelotii (Bail.) Kernels by UV spectrophotometry

Aboubakar Dandjouma AK. 2004. Contribution a l’étude des conditions de production et des aptitudes technologiques des huiles des oléagineux non – Conventionnels: cas de Ricinodendron heudelotii (Bail.) Pierre ex Pax et de Canarium schweinfurthii Engl.. PhD thesis, Université of Ngaoundere, Cameroun, 203p.

Aboubakar Dandjouma AK., Tchiegang C, Kapseu C, Linder M, Parmentier M. 2008. Enzyme-assisted hexane extraction of Ricinodendron heudelotii (Bail.) Pierre ex Pax seeds oil. International Journal of Food Science and Technology 43, 1169– 1175.

Bernal J, Mendiola JA, Ibánez E, Cifuentes A. 2011. Advanced analysis of nutraceuticals. Journal of Pharmaceutical  and  Biomedical  Analysis  55,  758–774.

Bernuy B, Meurens M, Mignolet E, Larondelle Y. 2008. Performance Comparison of UV and FT-Raman Spectroscopy in the Determination of Conjugated Linoleic Acids in Cow Milk Fat. Journal of Agricultural and Food Chemistry 56(4), 1159–1163.

Bernuy B, Meurens M, Mignolet E, Turu C, Larondelle Y. 2009. Determination by fourier transform raman spectroscopy of conjugated linoleic acid in i2-photoisomerized soybean oil. Journal of Agricultural and Food Chemistry 57, 6524-6527.

Cayuela Sánchez, JA., Moreda W,    García JM. 2013. Rapid Determination of Olive Oil Oxidative Stability and Its Major Quality Parameters Using Vis/NIR Transmittance Spectroscopy. Journal of Agricultural and Food Chemistry 61(34), 8056– 8062.

Codex Alimentarius. 1992. Programme mixte FAO/OMS sur les normes alimentaires ». FAO, Rome (Italie).

CODEX. 1999. Norme codex pour graisses et les huiles comestibles non visées par des normes individuelles ». Codex STAN 19-1981 (Rév. 2-1999), 5p.

El-Abassy RM, Donfack P, Materny A. 2009. Visible Raman spectroscopy for the discrimination of olive oils from different vegetable oils and the detection of adulteration. Journal of Raman Spectroscopy 40, 1284-1289.

Focant M, Mignolet E, Marique M, Clabots F, Breyne T, Dalemans D, Larondelle Y. 1998. The effects of vitamin E supplementation of cow diets containing rapeseed and linseed on the prevention of milk fat oxidation. Journal of Dairy Science 81, 1095-1101.

Igarashi M, Miyazawa T. 2005. Preparation and fractionation of conjugated trienes from alpha-linolenic acid and their growth inhibitory effects on human tumor cells and fibroblasts. Lipids 40, 109– 113.

International Union Of Pure And Applied Chemistry (IUPAC). 1979. Méthodes d’analyses des matières grasses et dérivées, 6 éme édition, Lavoisier Tec et Doc, Paris, France, 190p.

Kapseu C, Tchiegang C. 1995. Chemical properties of Ricinodendron heudelotii (Bail.) seed oil. Journal of Lipids 2, 87-98.

Karleskind A. 1996. Oils and Fats Manual. Vol. 1, Lavoisier TEC and DOC, Paris, France.

Kivçak B, Mert T. 2001. Quantitive determination of α-tocopherol in Arbutus unedo by TLC-densitometry and colorimetry. Fitoterapia 72, 656 –661.

Koba K, Imamura J, Akashoshi A, Kohno-Murase J, Nishizono S, Iwabuchi M, Tanaka K, Sugano M. 2007. Genetically modified rapeseed oil containing cis-9,trans-11,cis-13-octadecatrienoic acid affects body fat mass and lipid metabolism in mice. Journal of Agricultural and Food Chemistry. 55, 3741–3748.

Kohno H, Suzuki R, Noguchi R, Hosokawa M, Miyashita K., Tanaka T. 2002. Dietary conjugated linolenic acid inhibits azoxymethane-induced colonic aberrant crypt foci in rats. Jpn. Journal of Cancer Research 93, 133–142.

Kone S. 1998. Unités modernes de transformation des oléagineux à petite et très petite échelle: performances et limites . In C. Kapseu & G.J. Kayem (Eds.), Actes du 2éme Séminaire Safou, 3–5décembre 1997. Ngaoundéré, Cameroun: Presse Universitaires de Yaoundé I., 285–301p.

Lam CK, Chen J, Cao Y, Yang L, Wong YM, Yeung SY, Yao X, Huang Y, Chen ZY. 2008. Conjugated and nonconjugated octadecaenoic acids affect differently intestinal acyl coenzyme A: cholesterol acyltransferase activity. Atherosclerosis 198, 85–93.

Mosso K, Kouadio N, Koulibaly S, Kouman KG. 1998. Utilisations alimentaires des amandes du Ricinodendron heudelotii ssp heudelotii en Côte d’Ivoire. In: Kapseu C. et Kayem G.J. [eds.]. Actes du 2ème Séminaire Safou, 3-5 décembre 1997, Ngaoundéré (Cameroun), Presse Universitaires de Yaoundé I, 325-341. ISBN 2-911541-10-3.

Norris FA. 1982. Extraction of fats and oils. In D. Swern (Ed.), Baileyés industrial oils and fat products (Vol. 2, 4th ed.). New York (USA): John Wiley and Sons, 603p.

Orthoeffer T. 1995. Rice bran. Processing and nutritional considerations. Institute of Food Technologists Annual meeting, 7p.

Poulli KI, Mousdis GA. Georgiou CA.  2006. Synchronous fluorescence spectroscopy for quantitative determination of virgin olive oil adulteration with sunflower oil. Analytical and Bioanalytical Chemistry 386, 1571–1575.

Reaney MJT, Liu YD, Westcott ND. 1999. Commercial production of conjugated linoleic acid. In Advances in Conjugated Linoleic Acid Research, Press Champaim, IL 1, 39 -54. ISBN : 1-893997-02-2.

Schneider C. 2005. Review: chemistry and biology of vitamin E. Molecular Nutrition and Food Research 49, 7–30.

Suzuki R, Noguchi R, Ota T, Abe M, Miyashita K, Kawada T. 2001. Cytotoxic effect of conjugated trienoic fatty acids on mouse tumor and human monocytic leukemia cells. Lipids 36, 477–482.

Tchankou Leudeu BC. 2006. Etude sur des jeunes rats mâles des propriétés nutritionnelles des huiles de Canarium schweinfurthii Engl., Dacryodes edulis (G. Don) H. J. Lam, Ricinodendron heudelotii (Bail.) Pierre ex Pax, et Tetracarpidium conophorum Müll. Arg. Hutch. and Dalz. PhD thesis, Université of Ngaoundere, Cameroun, 259p.

Tchiegang C, Kapseu C, Ndjouenkeu R, Ngassoum MB. 1997. Amandes de Ricinodendron heudelotii (Bail.): Matière première potentielle pour les industries agro-alimentaires tropicales. Journal of Food Engineering 32, 1-10.

Tchiegang C, Ngo Oum M, Aboubakar Dandjouma AK, Kapseu C. 2004. Qualité et stabilité de l’huile extraite par pressage des amandes de Ricinodendron heudelotii (Bail.) Pierre ex Pax pendant la conservation à température ambiante. Journal of Food Engineering 62, 69-77.

Tchiegang C, Aboubakar Dandjouma A K, Kapseu C, Parmentier M. 2005. Optimisation de l’extraction de l’huile par pressage des amandes de Ricinodendron heudelotii Pierre ex Pax ». Journal of Food Engineering 68, 79–87.

Torrecilla JS, Rojo E, Dominguez JC, Rodriguez F. 2010. A Novel Method To Quantify the Adulteration of Extra Virgin Olive Oil with Low-Grade Olive Oils by UV-Vis. Journal of Agricultural and Food Chemistry 58, 1679-1679. http://dx.doi.org/10.1021/jf903308u.

Tsuzuki T, Igarashi M, Komai M, Miyazawa T. 2003. The metabolic conversion of 9,11,13-eleostearic acid (18:3) to 9,11-conjugated linoleic acid (18:2) in the rat. Journal of Nutritional Science and Vitaminology 49, 195–200.

Tsuzuki T, Tokuyama Y, Igarashi M, Nakagawa K, Ohsaki Y, Komai M. 2004. Alpha-eleostearic acid (9Z11E13E-18:3) is quickly converted to conjugated linoleic acid (9Z11E-18:2) in rats. Journal of Nutrition 134, 2634–2639.

Tsuzuki T, Kawakami Y. 2008. Tumor angiogenesis suppression by alpha-eleostearic acid, α linolenic acid isomer with a conjugated triene system, via peroxisome proliferator-activated receptor gamma. Carcinogenic. 29, 797–806.

Vivien J, Faure JJ. 1996. Fruitiers sauvages d’Afrique. Espèces du Cameroun. Coopération Française, CTA., 416 p.

Wiemar HJ, Altes KFW. 1993. Méthodes artisanales de transformation des oléagineux. Gate/GTZ, Eschborn (Allemagne Fédérale), 134p.

Yamasaki M, Kitagawa T, Koyanagi N, Chujo H, Maeda H, Kohno-Murase J, Imamura J, Tachibana H, Yamada K. 2006. Dietary effect of pomegranate seed oil on immune function and lipid metabolism in mice. Nutrition 22, 54–59.

Yang L, Leung KY, Cao Y, Huang Y, Ratnayake WM, Chen ZY. 2005. Alpha-linolenic acid but not conjugated linolenic acid is hypocholesterolaemic in hamsters. British. Journal of Nutrition 93, 433–438.

Yasui Y, Hosokawa M, Sahara T, Suzuki R, Ohgiya S, Kohno H, Tanaka T, Miyashita K. 2005. Bitter gourd seed fatty acid rich in 9 c, 11t – conjugated linolenic acid induces apoptosis and up-regulates the GADD45, p53 and PPARgamma in human colon cancer Caco-2 cells. Prostaglandins Leukotrienes and Essential Fatty Acids 73, 113–119.

Yuan GF, Sinclair AJ, Zhou CQ, Li D. 2009. Alpha eleostearic acid is more effectively metabolized into conjugated linoleic acid than punicic acid in mice. Journal of the Science of Food and Agricture 89, 1006-1011.


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