Content of total proteins, starch and proline in seeds of some wild relatives of cultivated plants

Paper Details

Research Paper 01/07/2018
Views (446) Download (15)

Content of total proteins, starch and proline in seeds of some wild relatives of cultivated plants

Badridze Gulnara, Chkhubianishvili Eva, Kikvidze Medea, Rapava Luara, Chanishvili Shota, Chigladze Lali, Goloshvili Tamar
J. Bio. Env. Sci.13( 1), 278-284, July 2018.
Certificate: JBES 2018 [Generate Certificate]


Revealing of interesting features among wild relatives of cultivated plants is prerequisite for their direct cultivation or application as a genetic material. Wild legumes and cereals of Georgia are not studied from this point of view. Accordingly, the purpose of the presented investigation was to study some, interesting from agricultural point of view, biochemical indices – content of total proteins, starch and proline, as well as total antioxidants activity – in seeds of some wild cereals and legumes. Plants species: Hordeum leporinum Link – mouse barley, Aegilops cylindrica Host – jointed goat grass, Avena fatua L. – common wild oat, Anisantha sterilis (L.) Nvsky- sterile brome, Poa palustris L. – fowl meadow grass, Agropyron repens (L.) P. Beauv.- quackgrass, and Pisum arvense (L.) Asch. – field pea served as test objects. Triticum aestivum L. – common wheat and Pisum sativum L. – pea were selected among the cultivated species for the comparative analysis of studied indices. Tested wild herbs may be grouped as interesting objects from agricultural and medicinal points of view, according to experimental results. Field pea and jointed goatgrass (seeds are rich of both starch and proteins), mouse barley and wild oat (seeds are rich of starch), as well as sterile brome (seeds contain enough amount of proteins) may be used in breeding and agricultural purposes, because of high content of proteins and starch in seeds. High content of proline in seeds and total antioxidant activity make wild oat, sterile brome, fowl meadowgrass and quack grass as interesting objects from medical point of view.


Bates LS, Waldren RP, Treare ID. 1973. Rapid determination of free proline for water-stress studies. Plant and Soil 39, 205-207.

Burrell MM. 2003. Starch: the need for improved quality or quantity an overview. Journal of Experimental Botany 54(382), 451–456.

Borzouei A, Kafi M, Akbari-Ghogdi E, Mousavi-Shalmani MA. 2012. Longterm salinity stress in relation to lipid peroxidation, superoxide dismutase activity and proline content of salt-sensitive and salt-tolerant wheat cultivars. Chilean journal of Agricultural Research 7(4), 476-482.

Cooper J, Moerkerk M. 2000. Bromus diandrus, Bromus rigidus. Weed ID/ Management. Australia.

Cooper R. 2015. Re-discovering ancient wheat varieties as functional foods. Journal of Traditional and Complementary Medicine 5, 138-143.

Donald WW and Ogg AG. 1991. Jr. Biology and Control of Jointed Goatgrass (Aegilops cylindrica). Weed Technology 5(1), 3-17.

Elias RJ, Kellerby SS, Decker EA. 2008. Antioxidant activity of proteins and peptides. Critical Reviews in Food Science and Nutrition 48(5), 430-41.

Fernandez-Marin B, Milla R, Martin-Robles N, Arc E, Kranner I, Becerril JM, Garcia-Plazaola JI. 2014. Side-effects of domestication: cultivated legume seeds contain similar tocopherols and fatty acids but less carotenoids than their wild counterparts. BMC Plant Biology 14, 1599. DOI: 10.1186/s12870-014-0385-1.

Ford-Lloyd BV, Schmidt M, Armstrong SJ, Barazani O, Engels J, Hadas R, Hammer K, Kell ShP, Kang D-M, Khoshbakht K, Li Y, Long Ch, 2011. Crop Wild Relatives-Undervalued, Underutilized and under Threat. Bioscience 61(7), 559-565.

Gubanov IA. 1990. Meadow herbs. Moscow. Agropromizdat. p32 (Russian).

Hamal A, Rzozi SB, Benbella M, Msatef Y, Bouhache M. 2001. Evaluation of the seed stock and dynamics of ripgut brome (Bromus rigidus Roth) in the cultivation of wheat in the Sais area of Morocco. Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen) 66(2b), 753-9.

INRA. 2001. HYPPA. Hypermedia for Plant Protection – Weeds. Dijon, France: Institut National de la Recherche Agronomique.

Johnston MB, Olivares AE, Calderón CE. 2009. Effect of quality and distribution of rainfall on Hordeum murinum L. growth and development. Chilean Journal of Agriculture Research. 69(2), 188-197.

KolevaII van Beek TA, Linssen JP, de Groot A, Evstatieva LN. 2002. Screening of plant extracts for antioxidant activity: A comparative study on three testing methods. Phytochemical Analysis 13, 8-17.

Lowry OH, Rosebrough NT, Farr AL, Randall RJ. 1951. Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 139, 256-275.

McKay B, Schatz GE. 2003. Field Pea Production. North Dakota State University.

Meyer RS, Duval AE, Jensen HR. 2012. Patterns and processes in crop domestication: An historical review and quantitative analysis of 203 global food crops. New Phytologist 196, 29-48.

Moerman D. 1998. Native American Ethnobotany. Timber Press. Oregon.

Moore M. 1989. Medicinal Plants of the Desert and Canyon West. publisher Museum of New Mexico Press. ISBN 978-089013182- 4, 129-131.

Pochinok KHN. 1976. Methods of Biological Analysis of Plants. Kiev, Naukova Dumka. 334 (in Russian).

Rowlands D, Frame DJ, Ackerley D, Aina T, Booth BBB, Christensen C, Collins M, Faull N, Forest CE, Grandey BS, et al. 2012. Broad range of 2050 warming from an observationally constrained large climate model ensemble. Nature Geoscience 5, 256-260.

Scholz H. 1991. The systematics of Avena sterilis and A. fatua (Gramineae). A critical study. Willdenowia 20(1-2),103-112.

Sharma P, Jha AB, Dubey RSh, Pessarakli M. 2012. Reactive Oxygen Species, Oxidative Damage, and Antioxidative Defense Mechanism in Plants under Stressful Conditions. Hindawi Publishing Corporation Journal of Botany 2012, Article ID 217037, 26 pages. DOI: 10.1155/2012/217037

Szabados L, Savoure A. 2009. Proline: a multifunctional amino acid. Trends in Plant Science 15(2), 89-97.

Tanaka T. 1976. Tanaka’s Cyclopaedia of Edible Plants of the World. Keigaku Publishing.

Tang ShX, Zhuang JJ, Wen YX, Ai SA, Li HJ, Xu J. 1997. Identification of introgressed segments conferring disease resistance in a tetrageneric hybrid of Triticum, Secale, Thinopyrum, and Avena. Genome 40(1), 99-103.

Vogl S, Picker P, Mihaly-Bison J, Fakhrudin N, Atanasov AG, Heiss EH, Wawrosch C, Reznicek G, Dirsch VM, Saukel J, Kopp B. 2013. Ethnopharmacological in vitro studies on Austria’s folk medicine–an unexplored lore in vitro anti-inflammatory activities of 71 Austrian traditional herbal drugs. Journal of Ethnopharmacology. 149(3), 750–71. DOI: 10.1016/j.jep.2013.06.007

Zhu F. 2017. Barley Starch: Composition, Structure, Properties, and Modifications. Comprehensive Reviews in Food Science and Food Safety 16, 558-579.