Creation of gene organisms in starting and ending regions of SicA gene of Salmonella enteritidis in pGEM vector
Paper Details
Creation of gene organisms in starting and ending regions of SicA gene of Salmonella enteritidis in pGEM vector
Abstract
Salmonella enteritidis is the most common cause of gastroenteritis. Sic A and Inv F activates transcription of sopB / sigD and sopE which coding effectively secreted proteins that enhance the code to be offensive. In this study upstream and downstream regions SicA gene of Salmonella enteritidis in pGEM vector was performed. In this study, the upstream and downstream genes SicA Salmonella enteritidis amplified based on specific primers and PCR and The fragment DNA cloned by T/A coloning method in pGEM vector and this constarct transformed into E. coli. The upstream and downstream SicA genes of Salmonella enteritidis was confirmed using PCR. The result of next steps showed that these regions were successfully cloned in E. coli. Confirmation of this conestract was done by digestion of restriction enzyme. According to the results ,can produce the conectruction via the recombination homologous and insertion of anti- biotic resistant genes between the upstream and downstream regions of SicA gene of Salmonella eneritidis as a candidate for gene vaccina against Salmonella eneritidis in future studies. Present study indicates the successful cloning of starting and ending encoder regions of SicA gene of Salmonella enteritidis in E. Coli bacteria. Thus, it seems that the structure produced in this study can be used as a gene vaccine candidate against Salmonella in future studies.
Bradley D, Jones. 2005. Salmonella Invasion Gene Regulation: A Story of Environmental Awareness. J Microbiology; 43(9), 110-117.
Chiu CH, Su LH, Chu C. 2004. Salmonella enterica serotype choleraesuis. epidemiologi, pathogenesis, clinical disease, and treatment. Clinical Microbiol Rev, 17(2), 311- 322.
Darwin KH, Miller V. 2001. Type III secretion chaperone-dependent regulation: activation of virulence genes by SicA and InvF in Salmonella typhimurium. EMBO Journal. 2(8), 1850-1862
Figueira R, Holden D. 2012. Functions of the Salmonella pathogenicity island 2(SPI-2) type III secretion system effectors. Microbiology.158, 1147– 1161.
Figueira R, Holden H. 2012. Functions of the Salmonella pathogenicity island 2 (SPI-2) type III secretion system effectors Microbiology,158, 1147– 1161.
Hueck CJ. 1998. Type III protein secretion systems in bacterial pathogens of animals and plants. Microbiol Mol Biol Rev. 62(2), 379- 433.
Jacobsen A, Hendriksen R, Aaresturp F, Ussery D, Friis C. 2011. The Salmonella enterica Pan-genom. Microbiol Ecolology, 62, 487–504.
Jones B. 2005. Salmonella Invasion Gene Regulation:A STORY OF Enviromental Awareness. Microbiology, 43, 110-117
Kaniga K, Tucker SC, Trollinger D, Gala´n JE. 1995. Homologues of the Shigella IpaB and IpaC invasinsare required for Salmonella typhimurium entry into culturedepithelial cells. Journal of Bacteriol. 177, 3965–3971
Ly KT, Casanova JE. 2007. Mechanisms of Salmonella entry into host cells. Cell Microbiol. 9(9), 2103- 2111
Sukhan A. 2000. The invasion-associated type III secretion system ofSalmonella typhimurium: common and unique features. CMLS. 57, 1033–1049.
Swearingen MC, Porwollik S, Desai P, McClelland M, Ahmer B. 2012.Virulence of 32 Salmonella Strains in Mice. PLoS ONE; 7(4), 1-5
Temme K, Salis H, Tullman-Ercek D, Levskaya A, Ho Hong SA. 2008. Voigt CH. Induction and Relaxation Dynamics of the Regulatory NetworkControlling the Type III Secretion System encoded within Salmonella Pathogenicity Island 1. J Molcular Biogical.; 377 (1), 47–61.
Tucker S, Gala´NJ. 2000. Complex Function for SicA, a Salmonella enterica SerovarTyphimurium Type III Secretion-Associated Chaperone. J Bacteriology.182(8), 2262–2268.
Zou M, Keelara SH, Thakur S. 2012. Molecular characterization of salmonella enterica serotype enteritidis isolates form humans by anti microbial resistance, virulence genes, and pulsed-field gl electrophoresis. Foodborne Pathog Dis. 9(3), 232-238.
Sara Ostovari, Mohammadreza Gholami, Mohammad Zolfaghari, Ghasem Rahimi, Morteza khani (2015), Creation of gene organisms in starting and ending regions of SicA gene of Salmonella enteritidis in pGEM vector; JBES, V6, N1, January, P665-669
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