Demutagenic potential of Brassica oleracea and its associated lactic acid bacteria

Paper Details

Research Paper 01/11/2019
Views (1040)
current_issue_feature_image
publication_file

Demutagenic potential of Brassica oleracea and its associated lactic acid bacteria

Vernalyn S. Doron, Lady Jane C. Fanuncio, Lucilyn L. Maratas
Int. J. Biosci. 15(5), 540-547, November 2019.
Copyright Statement: Copyright 2019; The Author(s).
License: CC BY-NC 4.0

Abstract

Cancer chemoprevention is a major cancer preventive strategy that utilizes naturally occurring dietary phytochemicals to inhibit the malignant transformation of initiated cells.  The consumption of cruciferous vegetables is claimed to be important in the prevention of cancerous diseases, and thus, the determination of the antimutagenicity of the edible vegetable, Brassica oleracea and its associated lactic acid bacteria is quite significant. All fresh cabbage samples yielded positive bacterial growth MRS agar plates while the rotten samples had none. Forty-two isolates were then presumptively identified as lactic acid bacteria after being subjected to cultural, morphological, and biochemical characterization. Potential demutagenic properties of suspensions of cabbage and lactic acid bacteria were assessed using the Ames test. No mutagenicity was found in the 10 cabbage suspensions to Salmonella typhimurium TA98 as well as that of the 42 lactic acid bacterial suspensions.

Ambrosone CB, Tang L. 2009. Cruciferous Vegetable Intake and Cancer Prevention: ole of Nutrigenetics. Cancer Prevention and Resistance (Philapa) 2(4), 298-300.

 Antwerpen EG. 1993. Cultivating Vegetables – Cabbage. Department of Agriculture, Forestry and Fisheries, Republic of South Africa. Retrieved from: https://www.daff.gov.za/daffweb3/Portals/0/InfoPaks/Vegetables%20-%20Cabbage.pdf

Abdull Razis AF, Noor NM. 2013. Cruciferous vegetables: dietary phytochemicals for cancer prevention. Asian Pacific Journal of Cancer Prevention 14(3), 1565-1570.

Bartoszek A, Baer-Dubowska W, Malejka-Giganti D. 2005. Carcinogenic and Anticarcinogenic Food Components. CRC Press.

Holt JG, Krieg NR, Sneath PHA, Stanley JT, William ST. 1994. Bergey’s Manual of Determinative Bacteriology. Williams and Wilikins, Baltimore.

Boubekri K, Ohta Y. 1996. Antimutagenicity of lactic acid bacteria from El-Klila cheese. Journal of the Science of Food and Agriculture. http://dx.doi.org/10.1002/(SICI)1097-0010(199612)72:4<397::AID-JSFA673>3.0.CO;2-E.

Buxton R. 2011. Nitrate and nitrite reduction test protocols. American Society for Microbiology. Retrieved from: http://www.asmscience.org/content/education/protocol/protocol.3660

Dinkova-Kostova AT, Kostov RV. 2012. Glucosinolates and isothiocyanates in health and disease. Trends in Molecular Medicine 18(6), 337–347. http://dx.doi.org/10.1016/j.molmed.2012.04.003

Falck K. 1987. Method for the performance of a mutagenicity test. United States Labsystems Oy (Helsinki, FI). Retrieved from: http://www.freepatentsonline.com/4675288.html

Gautam S, Saxena S, Kumar S. 2016. Fruits and Vegetables as Dietary Sources of Antimutagens. Journal of Food Chemistry and Nanotechnoly 2(3), 97114.

Kris-Etherton PM, Hecker KD, Bonanome A, Coval SM, Binkoski AE, Hilpert KF, Griel AE, Etherton TD. 2002. Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer. American Journal of Medicine 113, 9B, 71S-88S. http://dx.doi.org/10.1016/S0002-9343(01)00995-0 95.

Leboffe MJ, Pierce BE. 2006. Microbiology laboratory theory and application. 2nd Edition, Englewood, CO: Morton.

MacWilliams MP. 2009. Indole test protocol. American Science of Microbiology Retrieved from: https://www.asmscience.org/content/education/protocol/protocol.3202?crawler=true

Paganga G, Miller N, Rice-Evans CA. 1999. The polyphenolic content of fruit and vegetables and their antioxidant activities. What does a serving constitute? Free Radicals Research 30(2), 153-162. http://dx.doi.org/10.1080/10715769900300161.

Patel AD, Prajapati NK. 2012. Review on biochemical Importance of Vitamin K. Journal of Chemical and Pharmaceutical Research 4(1), 209-215.

Proteggente AR, Pannala AS, Paganga G, Van Buren L, Wagner E, Wiseman S, Van De Put F, Dacombe C, Rice-Evans CA. 2002. The antioxidant activity of regularly consumed fruit and vegetables reflects their phenolic and vitamin C composition. Free Radicals Research 36(2), 217-233. http://dx.doi.org/10.1080/10715760290006484.

Sun CH, Rokayya S, Li CJ, Zhao Y, Li Y. 2013. Cabbage (Brassica oleraceae L. var. capitata) Phytochemicals with Antioxidant and Anti-inflammatory Potential. Asian Pacific Journal of Cancer Prevention 14(11), 6657-6662. http://dx.doi.org/10.7314/APJCP.2013.14.11.6657

Thapa D, Zhang H. 2008. Lactobacillus rhamnosus exopolysaccharide reduces mutagenic potential of genotoxins. International Journal of Probiotics and Prebiotics 4(2), 79-82.

Touret T, Oliveira M, Semedo-Lemsadekk T. 2018. Putative probiotic lactic acid bacteria isolated from sauerkraut fermentations. PLoS One 13(9), e0203501. http://dx.doi.org/10.1371/journal.pone.0203501

Tse G, Eslick GD. 2014. Cruciferous vegetables and risk of colorectal neoplasms: a systematic review and meta-analysis. Nutrition and Cancer 66(1), 128–139. http://dx.doi.org/10.1080/01635581.2014.852686

Vijay U, Gupta S, Mathur P, Suravajhala P, Bhatnagar P. 2018. Microbial Mutagenicity Assay: Ames Test. Bio-protocol 8(6), e2763. http://dx.doi.org/10.21769/BioProtoc.2763

Woodman, H. 2003. Cabbage leaves: cabbage leaves are poor man’s poultice. BMJ 2003, 327. http://dx.doi.org/10.1136/bmj.327.7412.451-c.

Wu Y, Feng X, Jin Y, Wu Z, Hankey W, Paisie C, Li L, Liu F, Barsky, SH, Zhang W, Ganju R, Zou X. 2010. A novel mechanism of indole-3-carbinol effects on breast carcinogenesis involves induction of Cdc25A degradation. Cancer Prevention Research 3(7), 818–828. http://dx.doi.org/10.1158/1940-6207.CAPR-09-0213

Zahoor T, Rahman SU, Farooq U. 2003. Viability of Lactobacillus bulgaricus as yoghurt culture under different preservation methods. International Journal of Agriculture and Biology 5(1), 1560-8530.

Related Articles

Vegetable production systems in northern Côte d’Ivoire: Constraints, economic performance and integrated soil fertility management challenges

Marie Luce MÂ Semba Ouattara*, Jean Baptiste Gnelié Gnahoua, Kouassi Brahiman Kien, Kouamé Antoine N'Guessan, Yao Casimir Brou, Int. J. Biosci. 28(4), 41-50, April 2026.

Effect of temperature on the development of immature stages of Zeugodacus cucurbitae (Diptera: Tephritidae), Coquillett, 1899, A major watermelon pest in Senegal

Madeleine Ivonne Mendy*, Toffène Diome, Mamecor Faye, Mbacké Sembène, Int. J. Biosci. 28(4), 33-40, April 2026.

Structural characteristics of populations of Vitellaria paradoxa, Parkia biglobosa and Anacardium occidentale in rice-growing areas of the Tioroniaradougou subprefecture (Northern Côte d’Ivoire)

Dofoungo Koné*, Sibirina Soro, Seguena Fofana, Zana Silue, Doudjo Noufou Ouattara, Eboua Narcisse Wandan, Int. J. Biosci. 28(4), 23-32, April 2026.

In vitro anthelmintic efficacy of betel leaf (Piper betle) ethanolic extract against Ascaridia galli at varying concentrations

Elvin P. Dalida*, Mc Nelly V. Saraña, Zandro O. Perez, Int. J. Biosci. 28(4), 17-22, April 2026.

Perceptions, effectiveness, and credibility of artificial intelligence in healthcare among medical students and interns: A cross-sectional study

Shabeer Khan, Shafee Ur Rehman*, Naile Aleyna Dede, Mishal Tahir, Romana Hussain, Int. J. Biosci. 28(4), 8-16, April 2026.

A review on ethnomedicinal uses, phytochemical profile and pharmacological properties of Tetrastigma leucostaphylum (Dennst.) Alston ex Mabb.

P. P. Stephy, Prasobh K. Mohan, Anas Bin Firoz, S. Soosai Raj*, Int. J. Biosci. 28(4), 1-7, April 2026.