Determination of polyphenols, tannins, flavonoids and antioxidant activity in extracts of two genus Ircinia marine sponges of Atlantic Morrocan coast

Paper Details

Research Paper 01/12/2016
Views (265) Download (7)
current_issue_feature_image
publication_file

Determination of polyphenols, tannins, flavonoids and antioxidant activity in extracts of two genus Ircinia marine sponges of Atlantic Morrocan coast

Z. Rhandour, M. Tarbaoui, N. Sagou, M. Oumam, B. El Amraoui, A. Bennamara, A. Abourriche
J. Bio. Env. Sci.9( 6), 39-45, December 2016.
Certificate: JBES 2016 [Generate Certificate]

Abstract

Marine sponges are multicellular invertebrates that are part of the marine biomass from the Lower Cambrian period. They are present all over the planet and live in many aquatic ecosystems. It is found that the marine sponges are the source of the greatest number of metabolites isolated and characterized. These metabolites can beings present in high concentrations. This study is focused on the quantitative identification of organic substances such as: polyphenols, tannins, and flavonoids found in the extracts of two genus Ircinia marine sponges ‘spinulosa & oros’ of Atlantic Morrocan coast by various methods. The results of this study indicate that the extracts of Ircinia spinulosa are rich in flavonoids 51.48 (±2.10) (Eq mg Quercetin/mg extract) and in Tannins 0.205 (±0.001) (mg Cya/g dry matter), and for the polyphenols, the highest was found in the extract of Ircinia oros 15.75 (±0.95) (mg GAE/g dry matter).The evaluation of the antioxidant potential with using the radical scavenging capacity DPPH. test show that the extracts of the two sponges of the genus Ircinia spinulosa &Oros contain antioxidant activity respectively 28.25%, & 9.34% The antioxidant activity was observed in the crude extract of Ircinia spinulosa, which displayed a similar activity to synthetic BHT. Gas chromatography/mass spectroscopy (GC-MS) analysis of the extract revealed that the active compound was identical to synthetic BHT. The extracts sponges give good results, indicating that it possesses a significant amount of polyphenols, flavonoids, tannins, and an antioxidant activity.

VIEWS 3

Bakthavachalam B, Jiunn-Tzong W. 2008. Production of natural butylatedhydroxytoluene as an antioxidant by freshwater phytoplankton. International Journal Of Algal research 44, 1447–1454.

Benariba N, Djaziri R, Bellakhdar W, Belkacem N, Kadiata M, Malaisse WJ, Sener A. 2013. Phytochemical screening and free radical scavenging activity of Citrulluscolocyn this seeds extracts. Journal Tropical Biomedicine, 3, 35-40.

Benhammounabila N. 2011. Activité antioxydante des extraits des composes phénoliques de dix plantes médicinales de l’Ouest et du Sud-Ouest Algérien. PhD thesis, Université AboubakrBelkaïd-Tlemcen, Algeria 73-74.

Dhivya B, Arulanandu B, Arumugam V, Ramachandran S, Kandasamy D, Paramasivam N. 2015. Antioxidant Activity of Bacteria Associated with the Marine Sponge Tedaniaanhelans. Indian Journal of Microbiology 55, 13-18.

El-Amraoui B, Biard J-F, Uriz MJ, Rifai S, Fassouan A. 2010. Antifungal and antibacterial activity of Porifera extracts from the Moroccan Atlantic coasts. Journal of Medical Mycology20, 70-74.

Freitas AC, Rodrigues D, Rocha-Santos T, Gomes A, Duarte A . 2012. Marine biotechnology advances towards applications in new functional foods. Journal Of Biotechnology Advances30, 1506-1515.

Harvell D, Jordán-Dahlgren E, Merkel S, Rosenberg E, Raymundo L, Smith G, Weil E. 2007. Coral disease environmental drivers, and the balance between coral and microbial associates. Journal of Oceanography 20, 172-195.

Ngo DH, Vo TS, Ngo DN, Wijesekara I, Kim SK. 2012. Biological activities and potential health benefits of bioactive peptides derived from marine organisms. International Journal of Biological Macromolecules51, 378-383.

NIST Librery Nithyan P, Manju S, Karutha S. 2011. Phylogenetic characterization of culturable actinomycetes associated with the mucus of the coral Acropora digitifera from Gulf of Mannar. Journal of FEMS Microbiology Letters 314, 112-118.

Rhazi N, Oumam M, Hannache H, Sesboue A, Charrier B, Pizzic A, Charrier – El Bouhtouryb F. 2015. Comparison of the impact of different extraction methods on polyphenols yields and tannins extracted from Moroccan Acacia mollissima barks. Journal of Industrial Crops and Products 70, 245-252.

Singleton VL, Rossi JA. 1965. Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid reagents. Journal of Amenology and Viticulture 16, 144-158.

Sub C, Jang P, Mun P. 2007. Exploitation of polyphenol-rich pine barks for potent antioxidant activity. Journal of Wood Science 53, 524-528.

Trivedi NP, Rawal UM.2001. Hepatoprotective and antioxidant property of Andrographis paniculata (Nees) in BHC induced liver damage in mice. Journal Of Experimental Biology 39, 41-46.

Valliappan K, Sun WLZ.2014. Marine actinobacteria associated with marine organisms and their potentials in producing pharmaceutical natural products. Journal Microbiology and Biotechnology 98, 7365-77.

Yuanting Z, Qing L, Hang X, Xuefeng L, Longshan Z, Kankan Q, Kaishun B. 2013. Evaluation of antioxidant activity of ten compounds in different tea samples by means of an on-line HPLC–DPPH assay. Journal of Food Research International 53, I847-856.