Welcome to International Network for Natural Sciences | INNSpub

Effect of palm pollen grain, bee pollen grain and basil oil addition on hmf during honey storage

Research Paper | September 1, 2018

| Download 4

Abd-El-Rahman Metwaly, Hesham Sayed Ghazzawy, Mohammed Ahmad Bkarya, Iheb Chakroun, Mohammed Mohsen, Hamza Alamin, Samir Ghonim

Key Words:

Int. J. Biosci.13( 3), 114-119, September 2018

DOI: http://dx.doi.org/10.12692/ijb/13.3.114-119


IJB 2018 [Generate Certificate]


Honey is an important natural compound that is taken for food and medicine purposes and some additives can be used to increase its health effects and quality. In this research, we investigated the effect of addition palm pollen, bee pollen and basil oil under different storage temperatures on the formation of hydroxymethyl furfural (HMF) and pH. The treatments consist of adding the pollen grains (from 1 to 5%) and the oil (from 100 to 500 ppm) and storage them at different temperatures (30, 40 and 50°C) for one month, two months and three months. The quality was estimated by HMF determination by HPLC method and pH measurement. The storage temperature and best treatment for honey was at 30°C with addition of 2.5% and 5% palm pollen. Storage for one month is better than storage for two months at 40°C and also at 50°C. On the other hand, the pH increases from 4 to 6 with rising temperature and storage time. When adding pollen to honey, it is best to store at low temperature not exceeding 30°C. Also, the addition of basil oil has a better effect than pollen during storage


Copyright © 2018
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Effect of palm pollen grain, bee pollen grain and basil oil addition on hmf during honey storage

Almeida-Muradiana LB, Pamplonaa LC, Coimbraa S, Barthb OM. 2005:  Chemical composition and botanical evaluation of dried bee pollen pellets, Journal of Food Composition and Analysis 18(1), 105-111.

Antal Jm M, William SL, Geoffrey M, Richards N. 1990. Mechanism of formation of 5-(hydroxymethyl)-2-furaldehyde from d-fructose and sucrose, Carbohydrate Research 199, 91-109.

AOAC. 1990. Official Methods of Analysis, association of official Analytical chemists, Inc. suite 400-2200 Wilson Boulevard, USA.

Basumallick L, Rohrer J. 2017. Application note 270, Thermo Fisher Scientific, Sunnyvale, CA, USA, Determination of Hydroxymethyl furfural in Honey and Biomass.

Biesaga M. Pyrzynska K. 2013. Stability of bioactive polyphenols from honey during different extraction methods. Food Chem 136, 46–54.

Davidov-Pardo G, Arozarena Inigo Marın-Arroyo MR. 2011. Stability of polyphenolic extracts from grape seeds after thermal treatments. Eur. Food. Res. Technol 232, 211–220.

Hazem M, Hassan M. 2011. Chemical Composition and Nutritional Value of Palm Pollen Grains, Global Journal of Biotechnology & Biochemistry 6(1), 01-07.

Kmecl V, Maja I, Smodiš Š. 2014. A comparison of two methods for determination of HMF in honey and bee food: HPLC method versus spectrophotometric Winkler method, CAB Direct, Conference paper 49, 471-475.

Marotti M, Piccaglia R, Giovanelli M. 1996. Differences in Essential Oil Composition of Basil. Italian Cultivars Related to Morphological Characteristics J. Agric. Food Chem 44(12), 3926–3929.

Qamer S, Farooq A, Ali1 SS, Shakoori1 AA. 2013. Effect of Storage on Various Honey Quality Parameters of Apis dorsata Honey from Nepal, Pakistan J. Zoology 45(3), 741-747.

Schweitzer P. 2004: les critères de qualité du miel. Revue l’abeille de France N°916 Laboratoire d’analyse et d’écologie apicole.02.

Sultan A, Meoa S, AhmadAl-Asirib A, Latief M, Ansari MJ. 2017, Role of honey in modern medicine, Saudi Journal of Biological Sciences 24, Issue 5, 975-978,

Technical Sheet. 2016. Apiculture Promotion Center ADECAL. Techno pole Novel Caledonia 16.

White JW. 1994. The role of HMF and diastase assays in honey quality evaluation. Bee World 75(3), 104-117.