Effect of Pistacia atlantica Subsp. Kurdica essential oil and acetic acid on Botrytis cinerea growth in culture media, grape and cucumber fruits
Paper Details
Effect of Pistacia atlantica Subsp. Kurdica essential oil and acetic acid on Botrytis cinerea growth in culture media, grape and cucumber fruits
Abstract
The effect of Pistacia atlantica Subsp. Kurdica essential oil (EO) and acetic acid against Botrytis cinerea growth was investigated in synthetic media, cucumber and grape fruits. The antifungal assay was determined on PDA plates amended with thirty-three concentrations of this oil (250, 1000, 1500 to 26000 μι/ι). This study was conducted for acetic acid of 0.1% alone at concentrations of 5, 15 and 25%. Also 0.1% of each dilutions of acetic acid were added to a series of sterile molten PDA that contain different concentrations of essential oil ranging from 50 to 3000 μι/ι. EO inhibited radial growth on potato dextrose agar (PDA) in a dose-dependent manner. The growth was completely prevented by EO at 26000 ppm on PDA. In combination with 0.1% acetic acid 25% in fungal growth medium, minimum inhibitory concentration (MIC) of EO reduced to 3000 ppm. During 31 days of cold storage of cucumber and 60 days of grape at 4°C, the decay of these fruits caused by B. cinerea was reduced to 10% by using MIC of EO. Scanning electron microscopy (SEM) was done to study the mode of action of the oil in Botrytis cinerea and it was observed that treatment with the oil leads to distortion and thinning of the hyphal wall and the reduction in hyphal diameter. The results suggested the potential substitution of the antifungal chemicals by this EO and acetic acid as a natural inhibitor to control the growth of this mold in fruits such as cucumber and grape.
Behboodi BS. 2003. Ecological distribution study of wild pistachios for selection of rootstock. Options mediterran. 63, 61-66
Bellerbeck VG, De Roques CG, Bessiere JM, Fonvieille JL, Dargent R. 2001. Effect of Cymbopogon nardus (L) W. Watson essential oil on the growth and morphogenesis of Aspergillus niger. Canadian Journal of Microbiology 47, 9-17.
Corsetti A, Gobbetti M, Rossi J, Damiani P. 1998. Antimould activity of sourdough lactic acid bacteria: identification of a mixture of organic acids produced by Lactobacillus sanfrancisco CB1. Applied Microbiology and Biotetchnology 50, 253–256. http://dx.doi.org/10.1007/s002530051285
Davidson PM, Juneja VK. 1990. Antimicrobial agents. In: ‘Food Additives’. Branen, A.L.; Davidson, P.M. and Salminen S. (Eds), Marcel Dekker, Inc., New York, USA, pp. 83-137.
Gandomi H, Misaghi A, Akhondzadeh Basti A, Bokaei S, Khosravi A, Abbasifar A, Jebelli Javan A. 2009. Effect of Zataria multiflora Boiss. essential oil on growth and aflatoxin formation by Aspergillus flavus in culture media and cheese. Food and Chememical Toxicology 47, 2397–2400. http://dx.doi.org/10.1016/j.fct.2009.05.024
Gourama H. 1997. Inhibition of growth and mycotoxin production of Penicillium by Lactobacillusspecies. Lebensmittel-Wissenschaft & Technologie 30, 279–283.
Meepagala KM, Sturtz G, Wedge DE. 2002. Antifungal constituents of the essential oil fraction of Artemisia drancunculus L. var.dracunculus. J. Agricultural and Food Chemistry 50, 6989-6992.
Mihaliak CA, Gershenzo J, Croteau R. 1991. Lack of rapid monoterpene turnover in rooted plants, implications for theories of plants chemicals defenses. Oceologia 87, 373-376. http://dx.doi.org/10.1007/BF00634594
Moorman GW, Lease RJ. 1992. Benzimidazole-and dicarboximide-resistant Botrytis cinerea from Pennsylvania greenhouses. Plant Disease 76, 477-480.
Niku-Paavola, ML, Laitila A, Mattila-Sandholm T, Haikara A. 1999. New types antimicrobial compounds produced by Lactobacillus plantarum. Journal of Applied Microbiology 86, 29– 35. http://dx.doi.org/10.1046/j.1365-2672.1999.00632.x
Ormancey X, Sisalli S, Coutiere P. 2001. Formulation of essential oils in functional perfumery. Parfums, Cosmetiques, Actualites 157, 30-40.
Roller S. 2003. Natural antimicrobials for the minimal processing of foods. Woodhead Publishing Ltd., Cambridge, United Kingdom.
Sholberg PL, Conway WS. 2004. In: Agricultural Handbook Number 66: The Commerical Storage of Fruits, Vegetables and florist and Nursery Stocks, Gross K.C., C.Y. Wang and M. Saltveit (Eds), United States Department of Agriculture, Maryland, USA. Postharvest Pathology.
Tassou CC, Nychas GJE. 1995. Antimicrobial activity of the essential oil of mastic gum (Pistacia lentiscus var.chia) on gram positive and gram negative bacteria in broth and model food system. International Biodeterioration & Biodegradation 36, 411-20. http://dx.doi.org/10.1016/0964-8305(95)00103-4
Tian J, Ban X, Zeng H, He J, Huang B, Wan Y. 2011. Chemical composition and antifungal activity of essential oil from Cicuta virosa L. var.latisecta Celak. International Journal of Food Microbiology 145, 464–470. http://dx.doi.org/10.1016/j.ijfoodmicro.2011.01.023
Tripathi P, Dubey NK. 2004. Exploitation of natural products as an alternative strategy to control postharvest fungal rotting of fruit and vegetables. Postharvest Biology and. Technology 32, 235-245. http://dx.doi.org/10.1016/j.postharvbio.2003.11.005
Xia Z, Mao X, Luo Y. 1999.Study on antifungal mechanism of alpha-pinene. Hunan Yi Ke Da Xue Xue Bao 24, 507-9
Golnaz Hesami, Sadra Hesami, Adel Fatemi, 2013. Effect of Pistacia atlantica Subsp. Kurdica essential oil and acetic acid on Botrytis cinerea growth in culture media, grape and cucumber fruits. Int. J. Microbiol. Mycol., 1(2), 13-21.
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