Effect of sodium hypochlorite on in vitro sterilization of six promising potato cultivars
Paper Details
Effect of sodium hypochlorite on in vitro sterilization of six promising potato cultivars
Abstract
In vitro establishment of pathogen free, healthy germplasm for commercial and research purpose need asepsis. Each plant and type of explant has different protocol for disinfection. Excess amount of these sterilant can cause toxic affect. Therefore, this study was conducted to investigate the effect of different concentrations (0, 5, 10 and 15 %) and time duration (5, 10 and 15 minutes) of Sodium hypochlorite (NaOCl) on in vitro sterilization of potato explant to find out best sterilant dose with least toxic effect on in vitro culture establishment. The study shows that 10% NaOCl for 15 minutes proved significant sterilant while keeping low toxicity. It gave maximum number of healthy plants in each variety as well as minimum quantity of infected and non-growing culture with 92.2% healthy, 3.3% infected and 4.5% non-growing cultures for Kuroda, 91% healthy, 4.5% infected and 4.5% non-growing cultures for Astrex, 87.7% healthy, 5.6% infected and 6.7% non-growing cultures for Lady Rosetta, 92.2% healthy, 4.5% infected and 3.3% non-growing cultures for Sante, 94.5% healthy, 3.3% infected and 2.2% non-growing cultures for Diamant and 93.3% healthy, 4.5% infected and 2.2% non-growing cultures for Hermes. While higher doses eliminate the contaminants, but endure toxic effects on explant.
Ajayi SA, Berjak P, Kioko JI, Dullo ME, Vodouhe RS. 2006. Observation on In vitro Behaviour of the Zygotic Axes of Fluted Pumpkin. African Journal of Biotechnology 5(15), 1397-1404.
Altaf N. 2006. In vitro bud culture of kinnow tree. Pakistan Journal of Botany 38(3), 597-601.
Badoni A, Chauhan JS. 2010. In Vitro Sterilization Protocol for Micropropagation of Solanum tuberosum cv. ‘Kufri Himalini’. Academia Arena 2(4), 24. http://dx.doi.org/10.7537/marsaaj020410.03
Barrett C, Casselles AC. 1994. An evaluation of antibiotics for the elimination of Xanthomonas campestris cv. pelargonii (Brown) from Pelargonium x domesticum cv. ‘Grand Slam’ explants in vitro. Plant Cell Tissue and Organ Culture 36,169-175. http://dx.doi.org/10.1007/BF00037716
Buckley PM, Reed BM. 1994. Antibiotic susceptibility of plant associated bacteria. Horticultural Science 29(5), 434.
Bystron A, Sundqvist G. 1983. Bacteriologic evaluation of the effects of 0.5% sodium hypochlorite in endodontic therapy. Oral Surgery, Oral Medicine, Oral Pathology 55(3), 307-312. https://doi.org/10.1016/0030-4220(83)90333-X
Chawla HS. 2003. Plant Biotechnology: Laboratory manual for plant biotechnology. Oxford and IBH Publishing Co. Pvt. Ltd. New Delhi.
Colgecen H, Koca U, Toker G. 2011. Influence of different sterilization methods on callus initiation and production of pigmented callus in Arnebiaden siflora L. Turkish Journal Biology 35(4), 513–520. http://dx.doi.org/10.3906/biy-0911-161
Constantine DR. 1986. Micropropagation in the commercial environment. In: Withers, L. and P.G. Alderson (Eds.), Plant tissue culture and its agricultural applications. Butterworth, London, p. 175-186.
Estrela CRA, Barbin EL, Spano JCE, Marchesan MA, Pecora JD. 2002. Mechanism of Action of Sodium Hypochlorite. Brazilian Dental Journal 13(2), 113-117. http://dx.doi.org/10.1590/S010364402002000200007
FAO. 2008. International year of the potato. Fact Sheet. www.potato2008.org/en/potato/factsheets.html. Accessed 05 September 2016.
FAO. 2014. FAOSTAT. Statistical Database. Food and Agricultural Organization of United Nations, Rome, Italy. http://faostat3.fao.org/download/Q/QC/E.Accessed15 February 2016.
Fernie AR, Willmitzer L. 2001. Molecular and biochemical triggers of tuber development. Plant Physiology 127(4), 1459-1465. https://doi.org/10.1104/pp.010764
George F. 1993. Plant propagation by tissue culture. Exergetics Ltd., Edington, England. 574. http://dx.doi.org/10.1007/978-1-4020-5005-3
Goodwin PB, Kim YC, Adisarwanto T. 1980. Propagation of potato by shoot tip culture. Potato Research 23(1), 9-18. http://dx.doi.org/10.1007/BF02364021
Herman EB. 1996. Microbial contamination of plant tissue cultures. Agritech Consultants Inc, Shrub Oak, USA.
Hossain M, Ali MS, Rashid MM. 1994. Effect of inoculum levels of Potato virus Y (PVY) on yield and subsequent spread of the disease under insecticide spray and unsprayed condition. Bangladesh Journal. Botany 23, 87.
Hussain S, Lane SD, Lane DN. 1994. A preliminary evaluation of the use of microbial culture filtrates for the control of contaminants in plant tissue culture systems. Plant Cell Tissue and Organ Culture 36, 45-51. http://dx.doi.org/10.1007/BF00048314
Hussey G, Stacey NJ. 1981. In vitro propagation of potato (Solanum tuberosum L.). Annals of Botany 48(6), 787-796. https://doi.org/10.1093/oxfordjournals.aob.a086186
Mihaljevic I, Dugalic K, Tomas V, Viljevac M, Pranjic A, Cmelik Z, Puskar B, Jurkovic Z. 2013. In vitro sterilization procedures for micropropagation of ‘Oblacinska’ sour cherry. Journal of Agricultural Sciences, Belgrade 58, 117-126. http://dx.doi.org/10.2298/JAS1302117M
Miller SA, Lipschutz L. 1984. Potato in: Ammirato P.V., Evans, D., Sharp W. R. and Y. Yasuguki (Eds.), Handbook of plant tissue culture, McMillan publishing company New York. 3, 291-293.
Naik PS, Chandra R. 1993. Use of tissue culture technique in crop improvement with special reference to potato. CPRI, Shimla.
Odutayo OI, Oso RT, Akynyemi BO, Amusa NA. 2004. Microbial contaminants of cultured Hibiscus cannabinus and Telfaria occidentalis tissues. African Journal of Biotechnology 3(9), 473-476. http://dx.doi.org/10.5897/AJB2004.000-2092
Rabbani A, Askari B, Abbasi NA, Bhatti M, Quraishi A. 2001. Effect of growth regulators on in vitro multiplication of potato. International Journal of Agriculture & Biology 3(2), 181-182.
Roberts K. 1999. Why does chlorine kill plant? Accessed10 March 2016. http://www.madsci.org/posts/archives/jul99/932670591.Bt.r.html.
Rowe J. 2013. Understanding Sodium Hypochlorite. www.tpomag.com/editorial/2013/12/understanding_sodium_hypochlorite _wso.Accessed10 March 2016.
Villafranca MJ, Vermendi J, Sota V, Mingo-Castel AM. 1998. Effect of physiological age of mother tuber and number of subcultures on in vitro tuberization of potato (Solanum tuberosum L.), Plant Cell Reports 17, 787-790. http://dx.doi.org/10.1007/s002990050483
Wescott RJ, Henshaw GG, Roca WM. 1977. Tissue culture and storage of potato germplasm: Culture initiation and plant regeneration, Plant Science Letters 9, 309-315. https://doi.org/10.1016/0304-4211(77)90101-8
Yildiz M, Er C. 2002. The effect of sodium hypochlorite solutions on in vitro seedling growth and shoot regeneration of flax (Linum usitatissimum). Naturwissenschaften 89(6), 259-261. http://dx.doi.org/10.1007/s00114-002-0310-6
Zaman MS, Quershi A, Hassan G, Din RU, Ali S, Khabir A, Gul N. 2001. Meristem Culture of Potato (Solanum tubersum L.) for Production of Virus Free Plantlets. Journal of Biological Sciences 1(10), 898-899.
Abdul Ahad Qureshi, Nadeem Akhtar Abbasi, Ishfaq Ahmad Hafiz, Muhammad Kausar Nawaz Shah, 2017. Effect of sodium hypochlorite on in vitro sterilization of six promising potato cultivars. Int. J. Biosci., 11(2), 49-57.
Copyright © 2017 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.