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Ethnobotanical, phytochemical and nutritional characterization of Bixa Orellana Linn. seeds of Benin Ecology

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Research Paper 01/07/2020
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Ethnobotanical, phytochemical and nutritional characterization of Bixa Orellana Linn. seeds of Benin Ecology

Ezéchiel Akakpo, Marius Eric Badoussi, Claude Kouassi Gnacadja, Hermance Houngbo, Alphonse Dossou, Fernand Gbaguidi, Paulin Azokpota
Int. J. Biosci.17( 1), 46-56, July 2020.
Certificate: IJB 2020 [Generate Certificate]
Key: BeninBiocolorantFood industriesPharmacological propertiesSocio-cultural group

Abstract

Bixa orellana seeds, providers of biocolorant, are found in some localities of Benin. However, its local names, its uses such as the phytochemical and nutritional characteristics are not documented. This paper focuses on characterizing the ethnobotanical, phytochemical, and nutritional plan of Bixa orellana seeds of Benin ecology. An ethnobotanical investigation was first carried out close to 148 seeds users in two agro-ecological zones. The seeds collected at the investigation time were characterized through their phytochemical screening and evaluation of their nutritional value by reference methods. Various designations are associated to Annatto according to the socio-cultural groups of Benin such as “timati winiwini”, “timati kouinon” or “Kpokpo” (Fon and Mahi, in Zou and the Collines); “Kpararou”, “Dodokpara”, “Kparara” or “Timati borou” (Bariba and Dendi, in the North); “Disonri”, “Dipersonri” or “Monsonri” (Ditamari, in the North). These designations refer to its use in sauces as a substitute for tomatoes due to its red color (64.78%). Ripe fruit (10.81%) and seeds powder (8.10%) is used to treat anemia. Phytochemical screening of seeds revealed gallic tannins, flavonoids, anthocyanins, leucoanthocyanins, saponins, triterpenoids, steroids, mucilage, reducing compounds, and C-heterosides. Nutritionally, the seeds contain protein (12.83%), fats (4.64%), Potassium (14.59 mg/g), Sodium (6.93 mg/g), Phosphorus (4.79 mg/g), Calcium (2.89 mg/g), Magnesium (1.04 mg/g), Manganese (0.19 mg/g), Copper (0.19 mg/g), and Iron (0.01 mg/g). In addition to being an excellent source of dyes usable for various purposes, Annatto is a potentially nutritious product. It is therefore of paramount importance in the improvement of the quality of traditional food.

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Abhishek M, Rakshanda B, Prasad GBKS, Dua VK, Verma SK, Agarwal PK. 2010. Antimicrobial activity of plants traditionally used as medicines against some pathogens, Rasayan Journal of Chemistry 3, 615-620.

Adjatin A, Dansi A, Badoussi E, Loko YL, Dansi M, Azokpota P, Ahissou H, Akoègninou A, Akpagana K, Sanni A. 2013. Phytochemical screening and toxicity studies of Crassocephalum Rubens (Juss. ex Jacq.) S. Moore and Crassocephalum crepidioides (Benth.) S. Moore consumed as vegetable in Benin. International Journal of Current Microbiology and Applied Sciences 2, 1-13.

Akakpo E, Badoussi ME, Gnacadja KC, Houngbo H, Dossou A, Azokpota P. 2019. Le rocouyer (Bixa Orellana), une source de biocolorant pour les industries alimentaires: revue analytique. International Journal of Biological and Chemical Sciences 13, 2332-2351. https://dx.doi.org/10.4314/ijbcs.v13i4.36

Akoègninou A, Van der Burg WJ, Van der Measen LJG, Adjaèkidjè V, Sinsin B, Yédomonhan H. 2006. Flore Analytique du Bénin, Backhuys Publishers, 1034 p.

Alinnor IJ, Oze R. 2011. Chemical evaluation of the nutritive value of Pentaclethra macrophylla benth (African Oil Bean) Seeds. Pakistan Journal of Nutrition 10, 355-359.

AOAC. 1990. Official Methods of Analysis. (13th edn). Association of Official Analytical Chemists: Washington DC.

Assogbadjo AE, Chadare FJ, Glele-Kakaï R, Fandohan B, and Baidu-Forson JJ. 2012. Variation in biochemical composition of baobab (Adansonia digitata) pulp, leaves and seeds in relation to soil types and tree provenances. Agriculture, Ecosystems and Environment 157, 94– 99.

Badau MH, Abba HZ, Agbara GI, Yusuf AA. 2013. Proximate composition, mineral content and acceptability of granulated maize dumpling (Dambu Masara) with varying proportions of ingredients. Global Advanced Research Journal of Agricultural Science 2, 320-329.

Bangash JA, Arif M, Khan F, Khan F, Rahman AU, Iqbal H. 2011. Proximate composition, mineral and vitamin content of selected vegetables grown in Peshawar. Journal of the Chemical Society of Pakistan 33, 118-122.

Dansi A, Adjatin A, Adoukonou-Sagbadja H, Faladé V, Yedomonhan H, Odou D, Dossou B. 2008. Traditional leafy vegetables and their use in the Benin Republic. Genetic Resources and Crop Evolution 55, 1239-1256.

Das D, Maulik SR, Bhattacharya SC. 2007. Dyeing of wool and silk with Bixa orellana. Indian Journal of fibre & Textile Research 32, 366–372.

deAraújoVilar D, de Araujo Vilar MS, de Lima e Moura TFA, Raffin FN, Oliveira MR, de Oliveira Franco CF, de AthaydeFilho PF, FormigaMeloDiniz MF, Barbosa-Filho JM. 2014. Traditional Uses, Chemical Constituents, and Biological Activities of Bixa orellana L.: A Review. The Scientific World Journal 2014, p11. http://dx.doi.org/10.1155/2014/857292.

de Paula H, Pedrosa ML, Joamyr Victor RJ, Kenji Haraguchi F, dos Santos RC, Silva ME. 2009. Effect of an Aqueous Extract of Annatto (Bixa orellana) Seeds on Lipid Profile and Biochemical Markers of Renal and Hepatic Function in Hypercholesterolemic Rats. Brazilian Archives of Biology and Technology 52, 1373-1378.

Dike IP; Ibojo OO, Daramola FY, Omonhinmin AC. 2016. Phytochemical and Proximate Analysis of Foliage and Seed of Bixa orellana Linn. International Journal of Pharmaceutical Sciences Review and Research 36, 247-251.

Djè Y, Kouonon CL, Zoro Bi IA., Gnamien YG, Baudoin JP. 2006. «Etude des caractéristiques botaniques, agronomiques et de la biologie florale du melon africain (Cucumismelo L. var. agrestis Naudin, Cucurbitaceae)», Biotechnologie, Agronomie, Société et Environnement 10, 109 – 119.

Fagbohoun L. 2014. Etude chimique de colorants naturels et matériaux résineux traditionnels du Bénin dans le domaine artisanal. Thèse de doctorat de l’université d’Abomey-calavi (Bénin) et de l’université d’Avignon et des pays de Vaucluse (France) Spécialité: Chimie organique et substances naturelles. p 295.

Feldman KS, Sahasrabudhe K, Smith RS and Scheuchenzuber WJ. 1999. Immunostimulation by plant polyphenols. A relationship between tumor necrosis factor- alpha production and tannin structure. Biorganic and medical chemistry letters 9, 985-990.

Fleischer TC, Ameade EPK, Mensah MLK, Sawer IK. 2003. Antimicrobial activity of the leaves and seeds of Bixa orellana. Fitoterapia 74, 136-138.

Giorgi A, De Marinis P, Granelli G, Chiesa LM, Panseri S. 2013. Secondary Metabolite Profile, Antioxidant Capacity, and Mosquito Repellent Activity of Bixa orellana from Brazilian Amazon Region, Journal of Chemistry 1-10. http://dx.doi.org/10.1155/2013/409826.

Giuliano G, Rosati C, Bramley PM. 2003. To dye or not to dye: biochemistry of annatto unveiled. Trends in Biotechnology 21, 513-516. http://dx.doi.org/10.1016/j.tibtech.2003.10.001.

Gulrajani ML, Gupta G, Gupta P. 2002. Application of natural dyes on bleached in wool dyeing. Journal of the Textile Association 63, 119–124.

Houghton PJ, Raman A. 1998. Laboratory Handbook for the Fractionation of Natural Extracts, Chapman and Hall, New York, p 130-207. http://dx.doi:10.1007/978-1-4615-5809-5.

INSAE. (Institut National de la Statistique et de l’Analyse Economique), RGPH-4, 2013 (Recensement Général de la Population et de l’Habitation). 2016. Cahier des villages et quartiers de ville du département du Borgou, P 31.

Jansen PCM. 2005. Bixa orellana L. In: Jansen, P.C.M. & Cardon, D. (Editeurs). PROTA 3: Dyes and tannins/Colorants ettanins. [CD-Rom].

Kpètèhoto WH, Hessou S, Dougnon TV, Johnson RC, Boni G, Houéto EE, Assogba F, Pognon E, Loko F, Boko M, Gbénou J. 2017. Étude ethnobotanique, phytochimique et écotoxicologiquede Ocimum gratissimum Linn (Lamiaceae) à Cotonou, Journal of Applied Biosciences 109, 10609-10617. http://dx.doi.org/10.4314/jab.v109i1.5.

Lans CA. 2006. Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus. Journal of Ethnobiology and Ethnomedecin 2, p45.

Le Gall H, Philippe F, Domon JM, Gillet F, Pelloux J, Rayon C. 2015. Cell wall metabolism in response to abiotic stress. Plants 4, 112-66.

Morel S. 2011. Etude phytochimique et évaluation biologique de DerrisferrugineaBenth. (Fabaceae). Université d’Angers, thèse de doctorat, p 245.

Nair Archana G, Pradeesh S, DeviChinmayee M, Mini I, Swapna TS. 2013. Diplazium esculentum: A Wild Nutrient-Rich Leafy Vegetable from Western Ghats. Prospects in Bioscience 1, 293-301.

Omonhinmin AC, Dike IP, Agbara U. 2013. In vivo antioxidant assessment of two antimalarial plants Allamamda cathartica and Bixa orellana, Asian Pacific Journal of Tropical Biomedicine 3, 388-394.

Paumgartten F, De-Carvalho R, Araujo I, Pinto F, Borges O, Souza C, Kuriyama S. 2002. Evaluation of the developmental toxicity of annatto in the rat. Food and Chemical Toxicology 40, 1595-1601.

Periyasamy L, Kumar Y. 2016. GC-MS analysis and in-vitro cytotoxic studies of Bixa orellana seed extract against cancer cell line. International Journal of Pharmacy and Pharmaceutical Sciences 8, 1024-1036.

Prathima D, Sujitha A, Usha R. 2016. Phytochemical Screening and Antimicrobial Activity of Bixa Orellana Linn. International Journal of Pharmacognosy and Phytochemical Research 8, 1078-1082.

Rajib A, Monirul I, Israt JB, Ashik M, Ekramul H. 2009. Hepatoprotective Activity of methanol extract of some medicinal plants against carbon tetrachloride-induced hepatotoxicity in rats. European Journal of Scientific Research 37, 302-310.

Russell KR, Morrison EY, Ragoobirsingh D. 2005. The effect of annatto on insulin binding properties in the dog. Phytotherapy Research 19, 433-436.

Senga KP, Opota OD, Tamba VA, Tona LG, Kambu KO, Covaci A, Apers S, Pieters L, Cimanga K. 2013. Chemical composition and nutritive value study of the seed oil of Adenanthera pavonina L. (Fabaceae) growing in Democratic Republic of Congo. International journal of PharmTech Research 5, 205-216.

Senthil KP, Reddy YR, Ramesh S, Gobinath S, Ramana DBV. 2007. Evaluation of pigment extracted annatto seed (Bixa orellana) by chemical, in-vitro and in-saccotechniques in buffaloes. Buffalo Bulletin 26, 5-9.

Shilpi JA, Taufiq-Ur-Rahman MD, Uddin SJ, Alam MS, Sadhu SK, Seidel V. 2006. Preliminary pharmacological screening of Bixa orellana L. leaves. Journal of Ethnopharmacology 108, 264-271.

Valério MA, Lima R, Maria I, Braga N, Antônio J, Macedo R, Maria L. 2015. Annatto seed residue (Bixa orellana L.): nutritional quality. Food Science and Technology 35, 326-330. http://dx.doi.org/10.1590/1678-457X.6539

Venugopalan A, Giridhar P, Ravishankar GA. 2011. Food, ethnobotanical and diversified applications of Bixa Orellana L.: A scope for its improvement through biotechnological mediation. Indian Journal of Fundamental and Applied Life Sciences 1, 9-31.

Viuda-Martos M, Ciro-Gomez GL, Ruiz-Navajas Y. 2012. In vitro antioxidant and antibacterial activities of extracts from annatto (Bixa orellana L.) leaves and seeds, Journal of Food Safety 32, 399–406.

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