Evaluation of antibacterial and antioxidant activity of three plant species from Morus genus

Paper Details

Research Paper 01/02/2019
Views (332) Download (11)
current_issue_feature_image
publication_file

Evaluation of antibacterial and antioxidant activity of three plant species from Morus genus

Farhana Afroz Joty, Md. Mahmudul Hasan, Ratna Khatun, Md. Mutasim Billah, Md. Masum Miah, Shafi Mahmud, Md Abu Reza, Zennat Ferdousi
Int. J. Biosci.14( 2), 183-189, February 2019.
Certificate: IJB 2019 [Generate Certificate]

Abstract

Emergence and prevalence of antibiotic-resistant pathogenic bacteria and undesirable side effects of certain antibiotics have triggered immense interest in searching for new antimicrobial drugs of plant origin. Besides, the development of safer antioxidant, especially from plant source is also important to reduce oxidative stress related pathophysiological conditions. However, in this study, we aimed to evaluate antibacterial and antioxidant activity of the aqueous extracts of leaves of three different Morus species including M. indica (MI), M. latifolia (MLF), and M. laevigata (MLG).  Antibacterial activity was investigated against two pathogenic bacteria (E. coli and Pseudomonas) and three non-pathogenic bacteria (Acetobacter, RCA, and RVM).  The extracts were prepared at three different concentrations (0.25, 0.50, and 0.75 mg/disc) and the potency of the extracts was quantitatively assessed by the presence or absence of inhibition zone. Antioxidant potential of the three Morus species was evaluated using DPPH scavenging assay. All the three test plant possessed potent antibacterial and antioxidant capacity in a dose-dependent manner. The IC50 (dose at which 50% DPPH was scavenged) of MLF, MI, and MLG extracts were 67.21, 69.23, and 92.74 µg/mL, respectively. Among the three species, MI and MLF showed the highest antibacterial and antioxidant activity respectively. Overall, findings suggest that the three studied plant species of Morus genera are the important sources of pharmaceutical value.

VIEWS 19

Abdollahi M, Larijani B, Rahimi R, Salari P. 2005. Role of oxidative stress in osteoporosis. Therapy 2(5), 787-796.

Alade PI, Irobi ON. 1993. Antimicrobial activities of crude leaf extracts of Acalypha wilkesiana. Journal of Ethnopharmacology 39(3), 171-174.

Arabshahi-Delouee S, Urooj A. 2007. Antioxidant properties of various solvent extracts of mulberry (Morus indica L.) leaves. Food Chemistry 102(4), 1233-40.

Aruoma OI. 1998. Free radicals, oxidative stress, and antioxidants in human health and disease. Journal of the American Oil Chemist’s Society 75(2), 199-212.

Balandrin MF, Klocke JA, Wurtele ES, Bollinger WH. 1985. Natural plant chemicals: sources of industrial and medicinal materials. Science 228(4704), 1154-1160.

Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. 2012. Oxidative stress and antioxidant defense. World Allergy Organization Journal 5(1), 9.

Cohen ML. 1992. Epidemiology of drug resistance: implications for a post-antimicrobial era. Science 257(5073), 1050-1055.

Elmacı Y, Altuğ T. 2002. Flavour evaluation of three black mulberry (Morus nigra) cultivars using GC/MS, chemical and sensory data. Journal of the Science of Food and Agriculture 82(6), 632-635.

Eloff JN. 1998. Which extractant should be used for the screening and isolation of antimicrobial components from plants?. Journal of Ethnopharmacology 60(1), 1-8.

Giamarellou H. 2010. Multidrug-resistant Gram-negative bacteria: how to treat and for how long. International Journal of Antimicrobial Agents 36, S50-S54.

Hasan MM, Hasan R, Al Mahmud MR, Islam MG. 2018. Protective effect of Citrus macroptera fruit pulp juice rendering Nrf2-pathway against cisplatin-induced genotoxicity. Journal of Applied Pharmaceutical Science 8(12), 067-071.

Imran M, Khan H, Shah M, Khan R, Khan F. 2010. Chemical composition and antioxidant activity of certain Morus species. Journal of Zhejiang University Science B 11(12), 973-980.

Lakshmi P, Ganapaty S, Bharathi KM. 2013.  Chemical and biological examination of leaves of Morus indica. International Research Journal of Pharmacy 4(5), 173-177.

Leonard SS, Cutler D, Ding M, Vallyathan V, Castranova V,  Shi X. 2002. Antioxidant properties of fruit and vegetable juices: more to the story than ascorbic acid. Annals of Clinical & Laboratory Science 32(2), 193-200.

Pretorius JC, Magama S, Zietsman PC, Van Wyk BE. 2003. Growth inhibition of plant pathogenic bacteria and fungi by extracts from selected South African plant species. South African Journal of Botany 69(2), 186-192.

Rahman MO, Rahman MB, Siddiqui SA, Hassan MA, Rashid MA. 2018. Pharmacological evaluation of ethnomedicinal Glycosmis pentaphylla Lour. as antidiabetic, antioxidant and cytotoxic agent. Journal of Applied Pharmaceutical Science 8(11), 080-086.

Wilkins TD, Holdeman LV, Abramson IJ, Moore WEC. 1972. Standardized single-disc method for antibiotic susceptibility testing of anaerobic bacteria. Antimicrobial Agents and Chemotherapy 1(6), 451-459.