Evaluation of callus formation and embryogenesis in saffron (Crocus sativus L.) for flower harvesting

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Research Paper 01/01/2015
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Evaluation of callus formation and embryogenesis in saffron (Crocus sativus L.) for flower harvesting

Mirjalili Seyed Abbas and Poorazizi Elahe
J. Bio. Env. Sci.6( 1), 127-131, January 2015.
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Crocus sativus is belonging to Iridaceae. Due to its triploid nature it is sterile and is not able to set seeds, so it is propagated only by corms. Breeding and producing corms with free pathogens especially viruses is generally difficult; although vegetative production of the plant does easily by new corms. The aim was optimizing medium, hormones, sucrose level and temperature for embryogenesis and shoot elongation of saffron. Therefore, an experimental study was done in 7 treatments and 4 replications in two temperatures. Benzyl Adenopurine and 2,4-Dichlorophenoxy acetic acid were plant growth regulators. Data analyzed by statistical software. Results showed that the highest callus formation was in T4 containing 1 mg/lit of 2,4-D and 2 mg/lit BAP with the mean of 44.2 %. Also, the treatment with 45 gm/lit of sucrose produced the most length of shoot. The highest embryogenesis occurred in 4C.


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