Evaluation of the phytochemical, antioxidant and silver nanoparticles synthesized from the leaf extracts of Melia dubia and Carica papaya L. leaves

Paper Details

Research Paper 18/10/2024
Views (842)
current_issue_feature_image
publication_file

Evaluation of the phytochemical, antioxidant and silver nanoparticles synthesized from the leaf extracts of Melia dubia and Carica papaya L. leaves

A. Lakshmi, K. Kanimozhi, P. Prakash, V. Ambikapathy, A. Panneerselvam
Int. J. Biosci. 25(4), 308-319, October 2024.
Copyright Statement: Copyright 2024; The Author(s).
License: CC BY-NC 4.0

Abstract

Melia dubia and Carica papaya leaves contain a number of important medicinal compounds. In the current study, an effort was made to study the phytochemical analysis, antioxidant, anti-inflammatory, synthesized silver nanoparticles and antimicrobial activity of synthesized Ag-NPs from M. dubia and C. papaya leaf extract. The preliminary screening test is executed for the presence of secondary metabolites and was reported in order to understand the phytochemicals of leaf extract. Both qualitative and quantitative phytochemical constituents of alkaloids, aminoacids, carbohydrate, coumarins, flavonoids, phenol, protein, quinones, saponins, steroids, tannins, terpenoids and triterpenoids was analysed. Each extracted solvents is found to be recorded with carbohydrates and flavonoid contents. Studies on the antioxidant and anti-inflammatory activities of recognized phytochemicals present in plant leaves are also determined. The synthesized silver nanoparticles using the plant leaf extract of M. dubia and C. papaya are characterized by using UV-visible and their antimicrobial activities were performed. It was concluded that, Phytochemicals content presented in the leaf extracts are capable of rendering health benefits and thereby demonstrating that M. dubia and C. papaya is a potential medicinal plant and essentially replaced as a complement to synthetic anti-microbial agents. Especially, silver nanoparticles synthesized by the plant extracts lead a significant role in biological activities such as antimicrobial and antioxidant. The presence of significant plant leaf secondary metabolites contributes to its antioxidant and antimicrobial activity in future endeavor.

Aljuhani S, Rizwana H, Aloufi AS, Alkahtani S, Albasher G, Almasoud H, Elsayim R. 2024. Antifungal activity of Carica papaya fruit extract against Microsporum canis: in vitro and in vivo study. Front Microbial 15. https://doi.org/10.3389/fmicb.2024.1399671.

Anibijuwon II, Udeze AO. 2009. Antimicrobial activity of Carica papaya (pawpaw leaf) on some pathogenic organisms of clinical origin from South-Western Nigeria. Ethnobotanical Leaflets 13, 850–864.

Anyasor GN, Okanlawon AA, Ogunbiyi B. 2019. Evaluation of anti-inflammatory activity of Justicia secunda Vahl leaf extract using in vitro and in vivo inflammation models. Clinical Phytoscience 5, 49.

Banik B, Sahu N, Chetia N, Saikia M, Boruah P. 2017. Evaluation of antioxidant and antimicrobial activity of Carica papaya (Amita) leaf extracts. Current Trends in Pharmaceutical Research 4(1), 16–25.

Candra A, Fahrimal Y, Yusni Y, Azwar A, Santi TD. 2024. Phytochemistry and antifatigue activities of Carica papaya leaf from geothermal, coastal and urban areas, Indonesia. Narra J 4(1), e321.

Dagne E, Dobo B, Bedewi Z. 2021. Antibacterial activity of papaya (Carica papaya) leaf and seed extracts against some selected Gram-positive and Gram-negative bacteria. Pharmacogn J 13(6), 1727–1733.

Dhevishri S, Parameswari BD, Annapoorni H, Shankar MSS, Rajesh Kumar S. 2023. Antimicrobial properties of green synthesized silver and chitosan nanocomposites. Bioinformation 19(6), 745–748.

Dinesh B, Mahesh P, Shanthala M, Shalini V, Sindhu R, Viji KN, Rajashekara S. 2020. Evaluation of the phytochemical, antioxidant and antimicrobial activities obtained from the methanolic leaf extracts of Melia dubia Cav. Transactions on Science and Technology 7(4), 189–197.

Faridul HKM, Xiaoyi L, Shaoqin Z, Horvath PG, Bak M, Bejo L, Sipos G, Alpar T. 2022. Functional silver nanoparticles synthesis from sustainable point of view: 2000 to 2023 ‒ a review on game-changing materials. Heliyon 8(12), e12322.

Firdaus M, Andriana S, Elvinawati, Alwi W, Swistoro E, Ruyani A, Sundaryono A. 2017. Green synthesis of silver nanoparticles using Carica papaya fruit extract under sunlight irradiation and their colorimetric detection of mercury ions. International Symposium on Frontier of Applied Physics 817, 012029.

Gunasekaran K, Nirmala M, Raja K, Saravanakumar A. 2017. Characterization and application of biosynthesized silver nanoparticles from Melia dubia leaves. Indian Journal of Geo Marine Sciences 46(8), 1715–1720.

Harborne JB. 1992. Phytochemical methods: a guide to modern techniques of plant analysis. Chapman and Hall Publication, London, 22–26.

Krishna Kumar HN, Jyoti BC. 2019. Assessment of anti-arthritic, anti-inflammatory and antioxidant activity of Melia dubia. Journal of Pharmacognosy and Phytochemistry 8(3), 2579–2582.

Makkar HP, Norvsambuu T, Lkhagvatseren S, Becker K. 2009. Plant secondary metabolites in some medicinal plants of Mongolia used for enhancing animal health and production. Tropicultura 27, 159–167.

Mandal SDe, Lalmawizuala R, Vabeiryureilai M, Senthil Kumar N, Lalnunmawil E. 2015. An investigation of the antioxidant property of Carica papaya leaf extracts from Mizoram, Northeast India. 4(2), 43–46.

Maria GGP, Andrea RTB, Saul A, Martinez EM, Marchal JA, Boulaiz H, Raul AM. 2023. Scientific Reports 13, 790.

Nikkitha JP, Suresh P, Kameshwaran S, Rekha M, Mahendra Perumal G, Shanmugaiah V. 2021. Bioactive metabolites from ethyl acetate extract of leaves of Melia dubia L. against human and plant microbial pathogens. Journal of Advances in Microbiology Research 2(1), 13–21.

Pandey S, Cabot PJ, Shaw N, Hewavitharana AK. 2016. Anti-inflammatory and immunomodulatory properties of Carica papaya. Journal of Immunotoxicology 590, 590–602.

Samatha T, Shyamsundarachary R, Srinivas P, Swamy NR. 2012. Quantification of total phenolic and total flavonoid contents in extracts of Oroxylum indicum L. Kurz. Asian Journal of Pharmaceutical and Clinical Research 5, 177–179.

Singh P, Tanwar N, Saha T, Gupta A, Verma S. 2018. Phytochemical screening and analysis of Carica papaya, Agave americana and Piper nigrum. International Journal of Current Microbiology and Applied Sciences 7(2), 1786–1794.

Trung HT, Purnomo KA, Yu S-Y, Yang Z-J, Hu H-C, Hwang T-L, Tuan NN, Tu LN, Duc DX, Quang LD, Backlund A, Thang TD, Chang F-R. 2023. Anti-inflammatory and antiphytopathogenic fungal activity of 2,3-seco-tirucallane triterpenoids Melia dubins A and B from Melia dubia Cav. barks with ChemGPS-NP and in silico prediction. ACS Omega 8(40), 37116–37127.

Yadav RNS, Agarwala M. 2011. Phytochemical analysis of some medicinal plants. Journal of Phytology 3(12), 10–14.

Zeb A, Ullah F. 2016. A simple spectrophotometric method for the determination of thiobarbituric acid reactive substances in fried fast foods. Journal of Analytical Methods in Chemistry 9412767. https://doi.org/10.1155/2016/9412767.

Related Articles

Comparative responses of rice (Oryza sativa L.) to iron toxicity, drought and salinity stress: Morphological, physiological, biochemical and molecular regulation mechanisms

Yaya Touré*, Brahima André Soumahoro, Arthur Martin Affery, Tchoa Koné, Mongomaké Koné, Int. J. Biosci. 28(6), 37-50, June 2026.

Biocontrol potential of indigenous fungal antagonists from soils naturally suppressive to Fusarium oxysporum f. sp. cubense tropical race 4

Arnel V. Somolostro*, Carolina D. Amper, Mellprie B. Marin, Darwin M. Apistar, Myrna G. Ballentes, Ailyn Q. Daniel, Int. J. Biosci. 28(6), 27-36, June 2026.

Basal stem rot of oil palm in Africa: Emerging epidemiology, pathogen diversity and future management challenges

Emmanuel Fumbuka Mabula*, Agatha Aloyce, Alfonce Leonard, Pavithravani B. Venkataramana, Int. J. Biosci. 28(6), 13-26, June 2026.

The role of aberrant glycosylation in autoimmune disease development and progression

Md. Nafis Fuad Prottoy, Sayad Md. Didarul Alam*, Int. J. Biosci. 28(6), 1-12, June 2026.

Solvents’ influence on polyphenolic compound extractions from Lippia multiflora leaves (Mold, 1949), and their antioxidant activity

Kelemin Awa Koné*, Tagouèlbè Tiho, Mariam Sanogo, Casimir Kekou, Kouassi Hervé Tani, Int. J. Biosci. 28(5), 109-116, May 2026.

Economics of selected cropping system practices in the province of La Union, Philippines

Jennifer A. Cabading, V. Sagun Analyn, Angelina T. Gonzales*, Int. J. Biosci. 28(5), 97-108, May 2026.

The hidden burden: A review of toxicity from femoral orthopedic implants

Haroon Habib Beigh*, Nabeel Khan, Mirza Masroor Ali Beg, Int. J. Biosci. 28(5), 84-96, May 2026.