Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | March 1, 2022

| Download 20

GC/MS analysis and in vitro antioxidant and antibacterial activity of essential oil of Artemisia herba-alba Asso of Algeria

Mounira Kadri, Sana Goubi, Nasrine Salhi

Key Words:

Int. J. Biosci.20(3), 99-109, March 2022

DOI: http://dx.doi.org/10.12692/ijb/20.3.99-109


IJB 2022 [Generate Certificate]


The main objective of this study is to investigate the chemical composition, antioxidant and antimicrobial activities of the essential oil of Artemisia herba-alba Asso. of southern Algeria. Essential oil of Artemisia herba-alba Asso was extracted by hydrodistillation, and their chemical composition was identified by GC/MS Antioxidant activity of essential oil, has been done by using DPPH assay. The antimicrobial activity of essential oil was realised by the agar disc diffusion method. The essential oil extracted from the aerial parts by hydrodistillation was analysed by GC/MS. 39 constituents, representing 99.3% of the oil, were identified, of which the major ones, Thujone (12,759 %), Camphor (7,751%), Eacalyptol (4,525%), Isoborneol (1,119%). IC50 values observed for DPPH essay were 20,27 ±0,767 mg/ml. On the other hand, this oil was found effective against all tested strains; this activity ranged from 15.67±1.53 mm with Listeria innocua CIP 74915. These results provided evidence that the studied plant might indeed be a potential source of natural antioxidant and antimicrobial agents.


Copyright © 2022
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

GC/MS analysis and in vitro antioxidant and antibacterial activity of essential oil of Artemisia herba-alba Asso of Algeria

Abood S, Eichelbaum S, Mustafi S, Veisaga M L, López LA, Barbieri M. 2017. Biomedical Properties and Origins of Sesquiterpene Lactones, with a Focus on Dehydroleucodine. Natural Product Communications 12(6), 995-1005. https://doi.org/10.1177%2F1934578X1701200638

Akrout A, Gonzalez LA, El Jani HJ, Madrid PC. (2011). Antioxidant and antitumor activities of Artemisia campestris and Thymelaeahirsuta from southern of Tunisia. J. Food and Chemical Toxicology 49(2), 342–347. https://doi.org/10.1016/j.fct.2010.11.003

Akrout A. 2004. Essential oil study of some pastoral plants from Matmata (south Tunisia). Cahiers Options Méditerranéennes (CIHEAM). 62, 289-292. https://agris.fao.org/agris-search/search.do?recordID=QC2004600172

Aqil F, Khan MSA, Owais M, Ahmad I. 2005. Effect of certain bioactive plant extracts on clinical isolates of β-lactamase producing methicillin resistant Staphylococcus aureus. Journal of Basic Microbiology 45(2), 106–114. https://doi.org/10.1002/jobm.200410355

Bailey C, Danin A. 1981. Bedouin plant utilization in Sinai and Negev. Economic Botany 35(2), 145-162.

Belhattab R, Amor L, Barroso JG, Pedro LG, Figueiredo AC. 2014. Essential oil from Artemisia herba-alba Asso grown wild in Algeria: Variability assessment and comparison with an updated literature survey. Arabian Journal of Chemistry 7(2), 243-251. http://dx.doi.org/10.1016/j.arabjc.2012.04.042

Bertella A, Benlahcen KH, Abouamama S, Diana CGAP, Karim MK,  Kihal M, Artur MSS. 2018. Artemisia herba-alba Asso. Essential oil antibacterial activity and acute toxicity. Industrial Crops and Products 116, 137-143. https://doi.org/10.1016/j.indcrop.2018.02.064

Bezza L, Mannarino A, Fattarsi K, Mikail C, Abou L, HadjiMinaglou F, Kaloustian J.  2010. Chemical composition of the essential oil of Artemisia herba-alba issued from the district of Biskra (Algeria). Phytothérapie 8(5), 277-281. https://doi.org/10.1007/s10298-010-0576-3

Boukhris M, Regane G, Yangui T, Sayadi S, Bouaziz M. 2012.  “Chemical composition and biological potential of essential Oil from Tunisian Cupressus sempervirens L. Journal of Arid Land Studies 22(1), 329–332. http://nodaiweb.university.jp/desert/pdf/JALS-P74_329-332.pdf

Bruneton J. 1999. Pharmacognosie. Phytochimie. Plantes médicinales. 3ème édition. éd. TEC et DOC. Paris. 3 ème édition : Editions TEC & DOC Lavoisier, p 1120.

Carson CF, Hammer KA, Riley TV. 1995.  Broth micro-dilution method for determining the susceptibility of Escherichia coli and Staphylococcus aureus to the essentia1 oil of Melaleuca alternifolia (tea tree oil). Microbios 82, 181-185. https://www.ncbi.nlm.nih.gov/pubmed/7630326?report=abstract

Chalchat JC, Garry RPH, Harama M, Sidibe L.1995. Plantes aromatiques du Mali. Etude de deux Ocimum: O. basilicum et O. canum Sims. RivistaItaliana EPPOS. 204-214.

Cosentino S, Tuberoso CIG, Pisano B,  Satta M, Mascia V, Arzedi E , Palmas F. 1999. In-vitro antimicrobial activity and chemical composition of Sardinia Thymus essential oils. Letter in Applied. Microbiology 29, 130-135. http://onlinelibrary.wiley.com/doi/10.1046/j.1472-765X.1999.00605.x/pdf

Costa DC, Costa HS, Albuquerque TG, Ramos F, Castilho MC, Sanches-Silva A. 2015. Advances in phenolic compounds analysis of aromatic plants and their potential applications. Trends in Food Science & Technology 45(2), 336–354. http://dx.doi.org/10.1016%2Fj.tifs.2015.06.009

Dahmani-Hamzaoui N, Baaliouamer A. 2010. Chemical composition of Algerian Artemisia herba-alba essential oils isolated by microwave and hydrodistillation. Journal of Essential Oil Research. 22(6), 514-517. https://doi.org/10.1080/10412905.2010.9700386

El-massry K.  F, El-ghorab AH, Farouk A. 2002. Antioxidant activity and volatile components of Egyptian Artemisia judaica L. Food chemistry 79, 331–336. http://dx.doi.org/10.1016/S0308-8146(02)00164-4

Elmastas M, Isildak O, Turkekul I, Temur N. 2007.  Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms. Journal of Food Composition and Analysis 20, 337-345. http://dx.doi.org/10.1016/j.jfca.2006.07.003

Falleh H, Ksouri R, Chaieb K, Karray-Bouraoui N, Trabelsi N, Boulaaba M,  Abdelly C. 2008. Phenolic composition of Cynara cardunculus L. organs, and their biological activities, Comptes Rendus Biologies 331(5), 372-379. https://doi.org/10.1016/j.crvi.2008.02.008

Ghanmi M, Satrani B, Aafi A, Ismaili MR, Houtia H, Manfalouti H, Benchakroun K, Abarchane M, Harki L, Boukir A, Chaouch A,  Charrouf Z. 2010. Effet de la date de récolte sur le rendement, la composition chimique et la bioactivité des huiles essentielles de l’armoise blanche (Artemisia herba-alba) de la région de Guercif (Maroc oriental).Phytothérapie 8(5), 295-301. http://dx.doi.org/10.1007/s10298-010-0578-1

Griffin SG, Wyllie SG, Markham JL, Leach DN. 1999. The role of structure and molecular properties of terpenoids in determining their antimicrobial activity. Flavour and Fragrance Journal 14, 322-332 http://doi.org/10.1002/(SICI)10991026(199909/10)14:5%3C322::AID-FFJ837%3E3.0.CO;2-4

Guinoiseau E. 2010. Molécules antibactériennes issues d’huiles essentielles : séparation, identification et mode d’action. Thèse de doctorat pour l’obtention du grade de docteur de l’université de Corse. Ecole Doctorale Environnement et Société UMRCNRS 6134 SPE. p 148.

Harborne JB. 1998. Phytochemical methods. A guide to modern techniques of plants analysis. Third Edition. ISBN: 0-412-57260-5 (HB) and 0-412-57270-2 (PB).

Hatimi S, Boudouma M, Bichichi M, Chaib N, Idrissi NG. 2001. In vitro evaluation of antileishmania activity of Artemisia herba alba Asso. Bulletin de la société de pathologie exotique 94(1),  29-31.

Jouad H, Haloui M, Rhiouani H, Hilaly JE,  Eddouks M. 2001. Ethnobotanical survey of medicinal plants used for the treatment of diabetes, cardiac and renal diseases in the North centre region of Morocco (Fez–Boulemane). Journal of Ethnopharmacology. 77(2-3), 175-182. https://doi.org/10.1016/s0378-8741(01)00289-6

Kadri M, Salhi Nasrine Yahia A, Amiar K, Gnabzia H. 2017. Chemical composition, antioxidant and antimicrobial activities from extracts of Cymbopogon schoenanthus L. (spreng) of Algeria. International Journal of Biosciences | IJB 10(1), 318-326. http://dx.doi.org/10.12692/ijb/10.1.318-326

Karousou R, Koureas DN, Kokkini S. 2005. Essential oil composition is related to the natural habitats: Coridothymus capitatus and Satureja thymbra in NATURA 2000 sites of Crete. Phytochemistry 66(22), 2668-2673. https://doi.org/10.1016/j.phytochem.2005.09.020

Lattaoui   N, Tantaoui-elaraki A. 1994. Individual and combined antibacterial activity of the main components of three thyme essential oils. RevistaItaliana Eppos 13, 13-19.

Lopes-Lutz D, Alviano D, Alviono CS, Kolodziejczyk P. 2008. Screening of chemical composition, antimicrobial and antioxidant activities of Artemisia essential oils. Phytochemistry 69, 1732-1738. https://doi.org/10.1016/j.phytochem.2008.02.014

Mighri H, Hajlaoui H, Akrout A, Najjaa H, Neffati M. 2010. Antimicrobial and antioxidant activities of Artemisia herba-alba essential oil cultivated in Tunisian arid zone. Comptes Rendus Chimie 13(3), 291-386. https://doi.org/10.1016/j.crci.2009.09.008

Mouhamed Abou El-Hamd H, Magdi AE, Mohamed EH, Soleiman EH, Abeer ME, Naglaa SM. 2010. Chemical Constituents and Biological Activities of Artemisia herba-alba. Records of Natural Products 4(1), 1-25 https://www.acgpubs.org/RNP/2010/Volume%204/Issue%201/1_RNP-0906-111.pdf?q=mg-0906

Phaiphan  A, Baharin BS, Tan CP, Abdul Rahman R, Ganesan P. 2014. Antioxidant and antibacterial activities of different solvent extractions from Cassia siamea (Lamk.) leaves. Journal of Chemical and Pharmaceutical Research 6(4), 655-662.

Schinor EC, Salvador MJ, Ito IY, Dias DA. 2007. Evaluation of the antimicrobial activity of crude extracts and isolated constituents from Chrestascapigera. Brazilian Journal of Microbiology. 38, 145-149.

Shimada K, Fujikawa K, Yahara K, Nakamura T. 1992.Antioxidative properties of xanthone on the auto oxidation of soybean in cylcodextrin emulsion. Journal of agricultural and food chemistry (USA). 40, 945–948.

Shirwaikar A, Prabhu KS, Punitha ISR.  2006. In vitro antioxidant studies of phaeranthusindicus (Linn Indian Journal of Experimental Biology (IJEB). 44, 993-996. https://www.ncbi.nlm.nih.gov/pubmed/17176673

Sikkema J, De Bont JAM, Poolman B. 1994.Interactions of cyclic hydrocarbons with biological membranes. Journal of Biological Chemistry. 269(11), 8022-8028.

Singh A, Pratap-Singh A, Ramaswamy HS. 2016. A Controlled Agitation Process for Improving Quality of Canned Green Beans during Agitation Thermal Processing. Journal of Food Science 81(6), 1399–E1411. https://doi.org/10.1111/1750-3841.13308

Trease E, Evans WC. 1987. Pharmacognosie. Billiairetindall, london 13 th edition p 61-62.

Wang WM. 2004. On the Origin and Development of Artemisia (Asteraceae) in the Geological Past. Botanical Journal of the Linnean Society 145(3), 331–336. https://doi.org/10.1111/j.1095-8339.2004.00287.

Zaim A, El Ghadraoui L, Farah A. 2012. Effets des huiles essentielles d’Artemisia herba-alba sur la survie des criquets adultes d’Euchorthippus albolineatus (Lucas, 1849). Bulletin de l’Institut Scientifique, Rabat, section Sciences de la Vie. 34(2), 127-133. http://www.israbat.ac.ma/wpcontent/uploads/2015/01/17-Zaim_BIS_SV34_2_127.pdf

Zhang H, Chen F, Wang X, Yao HY. 2006. Evaluation of antioxidant activity of parsley (Petroselinum crispum) essential oil and identification of its antioxidant constituents. Food Research International 39, 833- 839. http://dx.doi.org/10.1016/j.foodres.2006.03.007


Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background