Total phenolic contents of two varieties of Crocus sativus and their antioxidant activity

Paper Details

Research Paper 01/03/2019
Views (1101)
current_issue_feature_image
publication_file

Total phenolic contents of two varieties of Crocus sativus and their antioxidant activity

Zafar Iqbal, Mohsan Javed, Samina Gull, Mian Habib-Ur-Rahman Mahmood, Zhang Hai
Int. J. Biosci. 14(3), 128-132, March 2019.
Copyright Statement: Copyright 2019; The Author(s).
License: CC BY-NC 4.0

Abstract

Methanolic extract of two varieties of crocus sativus dried stigmas was taken for total phenolic contents and antioxidant activity by using 1,1-diphenyl-2-picryl hydroxide scavenging. UV absorption of both samples separately showed three maximum wavelengths 236 nm and 234 nm (maximum absorbance of picrococine), 320 nm and 308 nm (maximum absorbance of safranal) and 436 nm and 434 nm (maximum absorbance of crocine) respectively. The electrochemical studies showed the oxidation reduction peaks. Total Phenolic content of red and brown saffron by using FC reagent was 5.05 mg and 4 mg of gallic acid equivalent/g of saffron extract respectively. At 50 µL concentration, both red and brown crocus sativus showed 71.68% and 73.70% inhibition respectively.

Basker D, Negbi M. 1999. The uses of saffron.Economic Botany 3, 228-236.

Espin JC, Soler-rivas C, Wichers HJ. 2000. Characterization o the total free radical scavenging capacity of vegetable oils and oil fractions using 2,2-diphenyl-1-picrylhydrazyl radical. Journal of Agriculture and Food Chemistry 48, 648-656.

Grilli CM. 2004. Saffron reproductive biology.Acta Horticulture650, 25-37.

Inayat A, Khan SR, Waheed A, Deeba F. 2010. Applications of eco-friendly natural dyes on leather using different modrants. Proceeding Pakistan Academy Science 47, 131-135.

Karimi E, Oskoueian E, Hendra R, Jaafar HZE. 2010. Evaluation of Crocus sativus L. stigma phenolic and flavonoid compounds and its antioxidant activity. Molecules 15, 6244–6256.

Kasparova S, Brezova V, Valko M, Horecky J, Mlynarik V,  Liptaj T, Dobrota D. 2005. Study of the oxidative stress in a rat model of chronic brain hypoperfusion. Neutochemistry International 46, 601-61.

Learsomrong K, Suttajit M, Chantiratikul P. 2009. Flow injection analysis system for the determination of total phenolic compounds by using folin-ciocalteu assay. Asian Journal of Applied Sciences 2, 184-190.

Lozano P, Castellar M, Simancas M, Iborra L. 1999. Quantitative high-performance liquid chromatographic method to analyse commercial saffron (Crocus sativus L.) products. Journal of Chromatography 830, 477-483.

Musa AE, Madhan B, Madhulatha W, Raghava RJ, Gasmelseed GA,  Sadulla S. 2009. Coloring of leather using henna-natural alternative material for dyeing. The Journal of the American Leather Chemists Association 104, 183-190.

Onem E, Gulumser GS, Ocak B. 2011. Evaluation of natural dyeing of leather with Rubia tinctorum Extract. Ekoloji Dergisi 20, 81-87.

Ordoudi SA, Befani CD, Nenadis N, Koliakos GG, Tsimidou MZ. 2009. Further examination of antiradical properties of crocus sativus stigmas extract rich in crocins. Journal of Agriculture Food Chemistry 57, 3080–3086.

Paschal FD, Danladi AA, Myek B, Sunday DJ, Joshua I, Ferdinand N. 2015.Extraction of dye stuff from Justicia carnea homeland its application in the dyeing of wool, leather and cotton, International Journal of Modern Chemistry 7, 81-90.

Plessner O, Negbi M, Ziv M, Basker D. 1989. Effects of temperature on the flowering of Crocus (Crocus sativus L.): Induction of hysteranthy. Israel Journal of Botany 38, 1-7.

Sanja SD, Sheth N, Patel D, Patel B. 2009. Characterization and evaluation of antioxidant activity of Portulaca oleracea. International Journal of Pharmacy and Pharmaceutical 1, 31-36.

Sivakumar V, Anna JL, Vijayeeswarri J, Swaminathan G. 2009. Ultrasound assisted enhancement in natural dye extraction from beetroot for industrial applications and natural dyeing of leather. Ultrasonics Sonochemistry 16, 782-789.

Nurul SR, Asmah R. 2012. Evaluation of antioxidant properties in fresh and pickled papaya. International Food Research Journal 19, 1117-1124.

Related Articles

Molecular detection and prevalence assessment of human norovirus: NoV (GI and GII) in Mugil cephalus (fish, teleost, Mugilidae) in Dakar, Senegal

Malick Ndiaye*, Malick Fall, Moussa Camara, Mamadou Malado Jallow, Ndongo Dia, Int. J. Biosci. 29(4), 144-152, October 2026.

Effects of partially replacing protein and fiber sources with cashew nut cake and cashew apple bagasse on the production performance of meat quails (Coturnix japonica Temminck & Schlegel, 1849)

N’Dri Saint-Clair Amani*, Moriféré Coulibaly, Gbohono Angelina Loukou, Howélé Ouattara, Int. J. Biosci. 29(4), 133-143, October 2026.

Mineral composition of fresh and dried peppers (Piper nigrum L.) from Côte d’Ivoire

Moussa Coulibaly*, Parfait Kouassi Kan Kouassi, Julien Koffi Golly, Doudjo Soro, Emmanuel Nogbou Assidjo, Benjamin Kouassi Yao, Int. J. Biosci. 29(4), 112-121, October 2026.

Phenotypic diversity of cocoa (Theobroma cacao L.) based on morphological characterization descriptors in major cocoa-growing areas of Tanzania

Godwin G. Maro*, Pavithravani B. Venkataramana, Kelvin M. Mtei, Mashamba Lucas Philip, Int. J. Biosci. 29(4), 102-111, October 2026.

Anti-inflammatory, analgesic and antipyretic activities of the aqueous leaf extract of Kalanchoe crenata (Andrews) Haw. traditionally used for ear disorders in Côte d’Ivoire

Monyn-Kouamé Ebalah Delphine*, Yéo Sounta Oumar, Sépé Gbodjrou Marthe, Kandé Brahima, Int. J. Biosci. 29(4), 81-89, October 2026.

General characteristics of the factors influencing the effectiveness of symbiotic relationships between legumes and bacteria

K. F. Bakhshaliyeva, N. D. İmamquliyev, M. İ. Qasımova, S. M. Muradova*, Int. J. Biosci. 29(4), 70-80, October 2026.