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Improving the viability of probiotics by encapsulation methods for development of functional dairy products

Review Paper | October 1, 2020

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Safeer Ahmad, Hafiz Nabeel Ahmad, Ghayyoor Ahmad, M. Saad Akram

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Int. J. Biomol. & Biomed.11( 2), 12-25, October 2020


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The popularity of functional foods among scientists and common people has been increasing day by day. Awareness and modernization make the consumer think better regarding food and nutrition. Now a day’s individual knows very well about the relation between food consumption and disease prevalence. Humans have a diversity of microbes in the gut that together form the gut microflora. Probiotics are the health-promoting live microbial cells improve host health through gut and brain connection and fighting against harmful bacteria. Bifidobacterium and Lactobacillus are the two bacterial genera which are considered to be probiotic. These good bacteria are facing challenges of viability. There are so many factors such as sensitivity to heat, pH, acidity, osmotic effect, mechanical shear, chemical components, freezing and storage time as well which affects the viability of probiotics in the dairy food matrix as well as in the gut. Multiple efforts have been done in the past and ongoing in present for these beneficial microbial population stability until their destination in the gut. One of a useful technique known as microencapsulation makes the probiotic effective in the diversified conditions and maintain these microbe’s community to the optimum level for achieving targeted benefits. Dairy products are found to be an ideal vehicle for probiotic incorporation. It has been seen that the encapsulated microbial cells show higher viability than the free cells in different processing and storage conditions as well as against bile salts in the gut. They make the food functional when incorporated, without affecting the product sensory characteristics.


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Improving the viability of probiotics by encapsulation methods for development of functional dairy products

Adhikari K, Mustapha A, Grün IU. 2003. Survival and metabolic activity of microencapsulated Bifidobacterium in stirred yogurt. Journal of Food Science 68(1), 275- 280.

Afzaal M, Saeed F, Arshad MU, Nadeem MT, Saeed M, Tufail T. 2019. The effect of encapsulation on the stability of probiotic bacteria in ice cream and simulated gastrointestinal conditions. Probiotics and Antimicrobial Proteins 11(4), 1348-1354.

Anal AK, Singh H. 2007. Recent advances in microencapsulation of probiotics for industrial applications and targeted delivery. Trends in Food Science and Technology 18(5), 240-251.

Appel-da-Silva MC, Narvaez GA, Perez LR, Drehmer L, Lewgoy J. 2017. Saccharomyces cerevisiae var. boulardii fungemia following probiotic treatment. Medical Mycology Case Reports 18, 15-17.

Bagchi T. 2014. Traditional food and modern lifestyle: Impact of probiotics. The Indian journal of Medical Research 140(3), 333.

Bakirtzi K, Law IKM, Xue X, Iliopoulos D, Shah YM, Pothoulakis C. 2016. Neurotensin promotes the development of colitis and intestinal angiogenesis via Hif-1α–miR-210 signaling. The Journal of Immunology 196 (10), 4311- 4321.

Begum PS, Madhav G, Rajagopal S, Viswanath B, Razak MA, Venkataratnamma V. 2017. Probiotics as functional foods: potential effects on human health and its impact on neurological diseases. International Journal of Nutrition, Pharmacology, Neurological Diseases 7 (2), 23.

Bergamini CV, Hynes ER, Quiberoni A, Suarez VB, Zalazar CA. 2005. Probiotic bacteria as adjunct starters: influence of the addition methodology on their survivalin a semi-hard Argentinean cheese. Food Research International 38, 597- 604.

Borgogna M, Bellich B, Zorzin L, Lapasin R, Cesàro A. 2010. Food microencapsulation of bioactive compounds: Rheological and thermal characterisation of non-conventional gelling system. Food Chemistry 122(2), 416- 423.

Boumis E, Capone A, Galati V, Venditti C, Petrosillo N. 2018. Probiotics and infective endocarditis in patients with hereditary hemorrhagic telangiectasia: a clinical case and a review of the literature. BMC Infectious Diseases 18(1), 65.

Boylston TD, Vinderola CG, Ghoddusi HB, Reinheimer JA. 2004. Incorporation of bifidobacteria into cheeses: challenges and rewards. International Dairy Journal 14(5), 375- 387.

Burgain J, Gaiani C, Linder M, Scher J. 2011. Encapsulation of probiotic living cells: From laboratory scale to industrial applications. Journal of Food Engineering. 104 (4), 467- 483.

Capela P, Hay TKC, Shah NP. 2006. Effect of cryoprotectants, prebiotics and microencapsulation on survival of probiotic organisms in yoghurt and freeze-dried yoghurt. Food Research International 39(2), 203-211.

Cayot P, Lorient DE. 1997. Structure-function relationships of whey proteins. Food Science and Technology-New York-Marcel Dekker 225- 256.

Champagne CP, Raymond Y, Guertin N, Bélanger G. 2015. Effects of storage conditions, microencapsulation and inclusion in chocolate particles on the stability of probiotic bacteria in ice cream. International Dairy Journal 47, 109-117.

Chavarri M, Maranon I, Ares R, Ibanez FC, Marzo F, Villaran MDC. 2010. Microencapsulation of a probiotic and prebiotic in alginate-chitosan capsules improves survival in simulated gastro-intestinal conditions. International Journal of Food Microbiology 142(1-2), 185-189.

Chen MJ, Chen KN. 2007. Applications of probiotic encapsulation in dairy products. Encapsulation and Controlled Release Technologies in Food Systems 83-107.

Chuayana EL, Ponce CV, Rivera RB, Cabrera EC. 2003. Antimicrobial activity of probiotics from milk products. Journal of Microbiology and Infectious Diseases 32(2), 71-74.

Clancy R. 2003. Immunobiotics and the probiotic evolution. FEMS Immunology and Medical Microbiology 38 (1), 9-12.

Collado MC, Isolauri E, Salminen S, Sanz Y. 2009. The impact of probiotic on gut health. Current Drug Metabolism 10(1), 68-78.

Cook MT, Tzortzis G, Khutoryanskiy VV, Charalampopoulos D. 2013. Layer-by-layer coating of alginate matrices with chitosan–alginate for the improved survival and targeted delivery of probiotic bacteria after oral administration. Journal of Materials Chemistry B 1(1), 52- 60.

Cross ML, Stevenson LM, Gill HS. 2001. Anti-allergy properties of fermented foods: an important immune regulatory mechanism of lactic acid bacteria? International Immunopharmacology 1(5), 891- 901.

De Vos P, Faas MM, Spasojevic M, Sikkema J. 2010. Encapsulation for preservation of functionality and targeted delivery of bioactive food components. International Dairy Journal 20(4), 292-302.

Dinakar P, Mistry VV. 1994. Growth and viability of Bifidobacterium bifidum in cheddar cheese. Journal of Dairy Science 77(10), 2854-2864.

Doleyres Y, Lacroix C. 2005. Technologies with free and immobilised cells for probiotic bifidobacteria production and protection. International Dairy Journal 15(10), 973- 988.

Fijan S. 2014. Microorganisms with claimed probiotic properties: an overview of recent literature. International Journal of Environmental Research and Public Health 11(5), 4745- 4767.

Fuller R. 1992. Probiotics. The Scientific Basis. 1st Ed. Chapman and Hall Ltd. London, UK. 398.

Gawkowski D, Chikindas ML. 2013. Non-dairy probiotic beverages: the next step into human health. Beneficial Microbes 4(2), 127-142.

Hansen LT, Allan-Wojtas PM, Jin YL, Paulson AT. Survival of Ca-alginate microencapsulated Bifidobacterium spp. in milk and simulated gastrointestinal conditions. Food Microbiology   19(1), 35-45.

Heidebach T, Forst P, Kulozik U. 2012. Microencapsulation of probiotic cells for food applications. Critical Reviews in Food Science and Nutrition 52(4), 291- 311.

Hoch C, Saad S. 2009. Viability of Lactobacillus acidophilus La-5 added solely or in co-culture with a yoghurt starter culture and implication on physico-chemical and related properties of Minas fresh cheese during storage. Food Science and Technology 42, 633- 640.

Holzapfel WH, Goktepe I, Juneja VK, Ahmedna M. 2006. Introduction to prebiotics and probiotics. Probiotics in Food Safety and Human Health 35(2), 109-116.

Huang R, Wang K, Hu J. 2016. Effect of probiotics on depression: a systematic review and meta-analysis of randomized controlled trials. Nutrients 8(8), 483.

Hyun CK, Shin HK. 1998. Utilization of bovine plasma obtained from a slaughterhouse for economic production of probiotics. Journal of Fermentation and Bioengineering 86(1), 34-37.

Jandhyala SM, Talukdar R, Subramanyam C, Vuyyuru H, Sasikala M, Reddy DN. 2015. Role of the normal gut microbiota. World Journal of Gastroenterology 21(29), 8787.

Jayalalitha V, Dorai RP, Dhanalakshmi B, Elango A, Kumar CN. 2011. Improving the viability of probiotics in yoghurt through different methods of encapsulation. Egyptian Journal of Dairy Science 39(1), 39- 44.

Kailasapathy K. 2009. Encapsulation technologies for functional foods and nutraceutical product development. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources 4 (33), 1- 19.

Kajander K, Hatakka K, Poussa T, Färkkilä M, Korpela R. 2005. A probiotic mixture alleviates symptoms in irritable bowel syndrome patients: a controlled 6-month intervention. Alimentary Pharmacology and Therapeutics 22(5), 387- 394.

Kalliomäki M, Salminen S, Arvilommi H, Kero P, Koskinen P, Isolauri E. 2001. Probiotics in primary prevention of atopic disease: a randomised placebo-controlled trial. The Lancet 357 (9262), 1076-1079.

Kanazawa H, Nagino M, Kamiya S, Komatsu S, Mayumi T, Takagi K, Asahara T, Nomoto K, Tanaka R, Nimura Y. 2005. Synbiotics reduce postoperative infectious complications: a randomized controlled trial in biliary cancer patients undergoing hepatectomy. Langenbeck’s Archives of Surgery 390 (2), 104- 113.

Karthikeyan N, Elango A, Kumaresan G, Gopalakrishnamurty TR, Pandiyan C. 2013. Augmentation of probiotic viability in ice cream using microencapsulationtechnique. International Journal of Advanced Veterinary Science and Technology 2(1), 76- 83.

Karthikeyan N, Elango A, Kumaresan G, Gopalakrishnamurty TR, Raghunath BV. 2014. Enhancement of probiotic viability in ice cream by microencapsulation. International Journal of Environmental Science and Technology 3(1), 339- 347.

Kerry RG, Patra JK, Gouda S, Park Y, Shin HS, Das G. 2018. Benefaction of probiotics for human health: A review. Journal of Food and Drug Analysis 26(3), 927- 939.

Kia EM, Alizadeh M, Esmaiili M. 2018. Development and characterization of probiotic UF Feta cheese containing Lactobacillus paracasei microencapsulated by enzyme based gelation method. Journal of Food Science and Technology 55(9), 3657- 3664.

Kołozyn-Krajewskaa D, Dolatowski ZJ. 2012. Probiotic meat products and human nutrition. Process Biochemistry 47(12), 1761-1772.

Korbekandi H, Mortazavian AM, Iravani S. 2011. Technology and stability of probiotic in fermented milks. Probiotic and Prebiotic Foods: Technology, Stability and Benefits to the Human Health 131- 169.

Krasaekoop W, Bhandari B, Deeth H. 2003. Evaluation of encapsulation techniques of probiotics for yoghurt. International Dairy Journal 13 (1), 3-13.

Krasaekoopt W, Bhandari B, Deeth H. 2004. The influence of coating materials on some properties of alginate beads and survivability of microencapsulated probiotic bacteria. International Dairy Journal 14(8), 737- 743.

Krishnan S, Kshirsagar AC, Singhal RS. 2005. The use of gum arabic and modified starch in the microencapsulation of a food flavoring agent. Carbohydrate Polymers 62(4), 309- 315.

Lin DC. 2003. Probiotics as functional foods. Nutrition in Clinical Practice 18(6), 497- 506.

Livney YD. 2010. Milk proteins as vehicles for bio actives. Current Opinion in Colloid and Interface Science 15(1-2), 73- 83.

Lopez-Rubio A, Gavara R, Lagaron JM. 2006. Bioactive packaging: turning foods into healthier foods through biomaterials. Trends in Food Science and Technology 17(10), 567-575.

Lourens-Hattingh A, Viljoen BC. 2001. Yogurt as probiotic carrier food. International Dairy Journal 11(1), 1-17.

Luckow T, Delahunty C. 2004. Which juice is ‘healthier’? A consumer study of probiotic non-dairy juice drinks. Food Quality and Preference 15(7), 751-759.

Madene A, Jacquot M, Scher J, Desobry S. 2006. Flavour encapsulation and controlled release–a review. International Journal of Food Science and Technology 41(1), 1- 21.

Marco ML, De Vries MC, Wels M, Molenaar D, Mangell P, Ahrne S, De Vos WM, Vaughan EE, Kleerebezem M. 2010. Convergence in probiotic Lactobacillus gut-adaptive responses in humans and mice. The ISME Journal 4(11), 1481-1484.

Martín MJ, Lara-Villoslada F, Ruiz MA, Morales ME. 2015. Microencapsulation of bacteria: A review of different technologies and their impact on the probiotic effects. Innovative Food Science and Emerging Technologies 27, 15- 25.

Mattila-Sandholm T, Myllarinen P, Crittenden R, Mogensen G, Fonden R, Saarela M. 2002. Technological challenges for future probiotic foods. International Dairy Journal 12(2), 173-182.

Messaoudi M, Lalonde R, Violle N, Javelot H, Desor D, Nejdi A, Bisson JF, Rougeot C, Pichelin M, Cazaubiel M, Cazaubiel JM. 2011. Assessment of psychotropic-like properties of a probiotic formulation (Lactobacillus helveticus R0052 and Bifidobacterium longum R0175) in rats and human subjects. British Journal of Nutrition 105(5), 755-764.

Mohammadi R, Mortazavian AM, Khosrokhavar R, da Cruz AG. 2011. Probiotic ice cream: viability of probiotic bacteria and sensory properties. Annals of Microbiology 61(3), 411- ­424.

Mohammadi R, Mortazavian AM. 2011. Technological aspects of prebiotics in probiotic fermented milks. Food Reviews International 27(2), 192- 212.

Mokarram RR, Mortazavi SA, Najafi MBH, Shahidi F. 2009. The influence of multistage alginate coating on survivability of potential probiotic bacteria in simulated gastric and intestinal juice. Food Research International 42(8), 1040- 1045.

Mortazavian AM, Azizi A, Ehsani MR, Razavi SH, Mousavi SM, Sohrabvandi, Reinheimer JA. 2008. Survival of encapsulated probiotic bacteria in Iranian yogurt drink (Doogh) after the product exposure to simulated gastrointestinal conditions. Milk Science International 63(4), 427- 429.

Mortazavian AM, Ehsani MR, Azizi A, Razavi SH, Mousavi SM, Sohrabvandi S, Reinheimer JA. 2008. Viability of calcium-alginate-microencapsulated probiotic bacteria in Iranian yogurt drink (Doogh) during refrigerated storage and under simulated gastrointestinal conditions. Australian Journal of Dairy Technology 63(1), 24-29.

Nejati R, Gheisari HR, Hosseinzadeh S, Behbod M. 2017. Viability of encapsulated Lactobacillus acidophilus (LA-5) in UF cheese and its survival under in vitro simulated gastrointestinal conditions. International Journal of Dairy Technology 70(1), 77- 83.

Ningtyas DW, Bhandari B, Bansal N, Prakash S. 2019. The viability of probiotic Lactobacillus rhamnosus (non-encapsulated and encapsulated) in functional reduced-fat cream cheese and its textural properties during storage. Food Control 100, 8- 16.

Nualkaekul S, Charalampopoulos D. 2011. Survival of Lactobacillus plantarum in model solutions and fruit juices. International Journal of Food Microbiology 146(2), 111- 117.

Oelschlaeger TA. 2010. Mechanisms of probiotic actions–a review. International Journal of Medical Microbiology 300(1), 57- 62.

Phillips M, Kailasapathy K, Tran L. 2006. Viability of commercial probiotic cultures (L. acidophilus, Bifidobacterium sp., L. casei, L. paracasei and L. rhamnosus) in cheddar cheese. International Journal of Food Microbiology 108(2), 276- 280.

Picot A, Lacroix C. 2004. Encapsulation of Bifidobacteria in whey protein-based microcapsules and survival in stimulated gastrointestinal conditions and in yoghurt. International Dairy Journal 14(6), 505-515.

Pinto SS, Fritzen-Freire CB, Dias CO, Amboni RD. 2019. A potential technological application of probiotic microcapsules in lactose-free Greek-style yoghurt. International Dairy Journal 97, 131- 138.

Poncelet D, Dreffier C. 2007. Microencapsulation methods from A to Z (or almost). Microencapsulation from Sciences to Technology 23-33.

Pradeep Prasanna PH, Charalampopoulos D. 2019. Encapsulation in an alginate–goats’ milk–inulin matrix improves survival of probiotic Bifidobacterium in simulated gastrointestinal conditions and goats’ milk yoghurt. International Journal of Dairy Technology 72(1), 132-141.

Prosapio V, Reverchon E, De Marco I. 2016. Production of lysozyme microparticles to be used in functional foods, using an expanded liquid antisolvent process. The Journal of Supercritical Fluids 107, 106-113.

Radulović Z, Miočinović J, Mirković N, Mirković M, Paunović D, Ivanović M, Seratlić S. 2017. Survival of spray‐dried and free‐cells of potential probiotic Lactobacillus plantarum 564 in soft goat cheese. Animal Science Journal 88(11), 1849- 1854.

Ranadheera CS, Evans CA, Adams MC, Baines SK. 2012. In vitro analysis of gastrointestinal tolerance and intestinal cell adhesion of probiotics in goat’s milk ice cream and yogurt. Food Research International 49(2), 619-625.

Rasic JL. 2003. Microflora of the intestine probiotics. In B.Caballero, L. Trugo, and P. Finglas (Eds.). Encyclopedia of Food Sciences and Nutrition. Oxford: Academic Press. 3911-3916

Rayes N, Seehofer D, Theruvath T, Schiller RA, Langrehr JM, Jonas S, Bengmark S, Neuhaus P. 2005 . Supply of pre-and probiotics reduces bacterial infection rates after liver transplantation—a randomized, double-blind trial. American Journal of Transplantation 5(1), 125-130.

Reid G. 2015. The growth potential for dairy probiotics. International Dairy Journal 49, 16- 22.

Reyes V, Chotiko A, Chouljenko A, Sathivel S. 2018. Viability of Lactobacillus acidophilus NRRL B-4495 encapsulated with high maize starch, maltodextrin, and gum arabic. LWT 96, 642- 647.

Rivera-Espinoza Y, Gallardo-Navarro Y. 2010. Non-dairy probiotic products. Food Microbiology 27(1), 1-11.

Roberfroid MB. 1999. What is beneficial for health? The concept of functional food. Food Chemistry and Toxicology 37, 1039-1041.

Rosenfeldt V, Benfeldt E, Valerius NH, Pærregaard A, Michaelsen KF. 2004. Effect of probiotics on gastrointestinal symptoms and small intestinal permeability in children with atopic dermatitis. The Journal of Pediatrics 145(5), 612-616.

Ross RP, Fitzgerald G, Collins K, Stanton C. 2002. Cheese delivering biocultures: probiotic cheese. Australian Journal of Dairy Technology 57, 71- 78.

Sajilata MG, Singhal RS, Kulkarni PR. 2006. Resistant starch–a review. Comprehensive Reviews in Food Science and Food Safety 5 (1), 1-17.

Salem MM, Fathi FA, Awad RA. 2005. Production of probiotic ice cream. Polish Journal of Food and Nutrition Sciences 14(3), 267.

Santacruz S, Castro M. 2018. Viability of free and encapsulated Lactobacillus acidophilus incorporated to cassava starch edible films and its application to Manaba fresh white cheese. LWT Food Science and Technology 93, 570- 572.

Semyonov D, Ramon O, Kaplun Z, Levin-Brener L, Gurevich N, Shimoni E. 2010. Microencapsulation of Lactobacillus paracasei by spray freeze drying. Food Research International 43(1), 193-202.

Sendra E, Fayos P, Lario Y, Fernández-López J, Sayas-Barberá E,Pérez-Alvarez JA. 2008. Incorporation of citrus fibers in fermented milk containing probiotic bacteria. Food Microbiology 25(1), 13-21.

Shah NP, Ravula R. 2004. Selling the cells in desserts. Dairy IndustriesInternational 69(1), 31-32.

Siro I, Kapolna E, Kapolna B, Lugasi A. 2008. Functional food. Product development, marketing and consumer acceptance-A review. Appetite 51(3), 456-467.

Sohail A, Turner MS, Coombes A, Bostrom T, Bhandari B. 2011. Survivability of probiotics encapsulated in alginate gel microbeads using a novel impinging aerosols method. International Journal of Food Microbiology 145(1), 162- 168.

Solanki HK, Pawar DD, Shah DA, Prajapati VD, Jani GK, Mulla AM, Thakar PM. 2013. Development of microencapsulation delivery system for long-term preservation of probiotics as biotherapeutics agent. Biomedical Research International 2013, 21.

Song H, Yu W, Gao M, Liu X, Ma X. 2013. Microencapsulated probiotics using emulsification technique coupled with internal or external gelation process. Carbohydrate polymers 96(1), 181- 189.

Stanton C, Gardiner G, Meehan H, Collins K, Fitzgerald GF, Lynch PB, Ross RP. 2001. Market potential for probiotics. The American Journal of Clinical Nutrition 73(2), 476- 483.

Sultana K, Godward G, Reynolds N, Arumugaswamy R, Peiris P, Kailasapathy K. 2000. Encapsulation of probiotic bacteria with alginate—starch and evaluation of survival in simulated gastrointestinal conditions and in yoghurt. International Journal of Food Microbiology 62 (1-2), 47- 55.

Suvarna VC, Boby VU. 2005. Probiotics in human health: A current assessment. Current Science 88(11), 1744- 1748.

Tripathi MK, Giri SK. 2014. Probiotic functional foods: Survival of probiotics during processing and storage. Journal of Functional Foods 9, 225- 241.

Tsai YL, Lin TL, Chang CJ, Wu TR, Lai WF, Lu CC, Lai HC. 2019. Probiotics, prebiotics and amelioration of diseases. Journal of Biomedical Science 26 (1), 1- 8.

Tuomola EM, Ouwehand AC, Salminen SJ. 2006. The effect of probiotic bacteria on the adhesion of pathogens to human intestinal mucus. FEMS Immunology and Medical Microbiology 26 (2), 137- 142.

Ventura M, Perozzi G. 2011. Probiotic bacteria and human gut microbiota. Genes and Nutrition 6(1), 203-204.

Verbeke W. 2005. Consumer acceptance of functional foods: socio-demographic, cognitive and attitudinal determinants. Food Quality and Preference 16 (1), 45- 57.

Vinderola CG, Reinheimer JA. 2003. Lactic acid bacteria: A comparative “in vitro” study of probiotic characteristics and biological barrier resistance. FoodResearch International 36 (9-10), 895- 904.

Wattananapakasem I, van Valenberg HJ, Fogliano V, Costabile A, Suwannaporn P. 2018. Synbiotic microencapsulation from slow digestible colored rice and its effect on yoghurt quality. Food and Bioprocess Technology 11(6), 1111-1124.

Yoon WB, Mccacarthy KL. 2002. Rheology of yogurt during pipe flow as characterized by magnetic resonance imaging. Journal of Texture Studies 33(5), 431- 444.

Zanjani MAK, Ehsani MR, Ghiassi Tarzi B, Sharifan A. 2018. Promoting Lactobacillus casei and Bifidobacterium adolescentis survival by microencapsulation with different starches and chitosan and poly L-lysine coatings in ice cream. Journal of Food Processing and Preservation 42(1), 13318.

Zinedine A, Faid M. 2007. Isolation and characterization of strains of Bifidobacteriawith probiotic proprieties in vitro. World Journal of Dairy and Food Science 2(1), 28-34.

Zuidam NJ, Shimoni E. 2009. Overview of microencapsulates for use in food products or processes and methods to take them. Encapsulation Technologies for Active Food Ingredients and Food Processing 3-29.