In silico identification of NaCl responsive genes in apple

Paper Details

Research Paper 01/09/2017
Views (367) Download (10)
current_issue_feature_image
publication_file

In silico identification of NaCl responsive genes in apple

Pouya Gharbi, Nasser Mahna, Nader Farsad-Akhtar, Majid Norouzi
Int. J. Agron. Agri. Res.11( 3), 88-96, September 2017.
Certificate: IJAAR 2017 [Generate Certificate]

Abstract

Salinity is one of the major abiotic stresses, limiting the productivity of agricultural crops. The present research designed to find and evaluate sodium chloride-responding genes in Malus domestica which have homologs in Arabidopsis thaliana. We found in the literature that there are 112 Arabidopsis proteins categorized in 28 osmotic stress responsive groups and performed a comparative analysis against Malus domestica database in GDR and eventually could identify 212 Malus domestica sequences. These genes were identified according to the high similarity percentage, high consequently alignment score, low E-value and the pattern sequence coverage. The analysis indicated a high number of sequences and diverse genes from all mentioned categories in apple. One of the most frequent ones came up with unknown function. Analyzing these sequences with an unknown function lead to the annotation of 45 Malus domestica sequences. By collecting a bunch of genes probably having a function in salt or osmotic stress responses in apple, we could have a better understanding about how a plant in Rosaceae can survive in salt stress condition.

VIEWS 12

Anonymous. 2016. United States Department of Agriculture Agricultural Research Service, Research www. ars.usda.gov.

Anonymous. 2017. Genome Database for Rosaceae, www.rosaceae.org/species/malus/malus_x_domestica

Apse MP, Aharon GS, Snedden WA, Blumwald E. 1999. Salt tolerance conferred by overexpression of a vacuolar Na+/H+ antiport in Arabidopsis. Science 285, 1256-1258.

Chen S, Tarsio M, Kane PM, Greenberg ML. 2008. Cardiolipin mediates cross talk between mitochondria and the vacuole. Molecular Biology of the Cell 19, 5047- 5058. 10.1091/mbc.E08-05-0486.

Claverie JM, Notredam C. 2007. Bioinformatics for dummies. 2nd edition. Wiley publishing.

Davenport R. 2007. The Na+ transporter ; contorols retrieval of Na+ from the xylem in Arabidopsis thaliana. Plant, Cell & Environment 30, 497-507.

Dietz KJ, Vogel MO, Viehhauser A. 2010. AP2/EREBP transcription factors are part of gene regulatory networks and integrate metabolic, hormonal and environmental signals in stress acclimation and retrograde signaling. Protoplasma 245, 3-14.

Ferree DC, Warrington IJ. 2003. Apples: Botany, production and uses. CABI; First edition.

Gaxiola RA, Palmgren MG, Schumacher K. 2007. Plant proton pumps. FEBS Letters 581, 2204-2214.

Gaxiola RA. Jisheng L, Undurraga S, Dang L, Allen G, Alper SL, Fink GR. 2001. Drought and salt tolerant plants result from overexpression of the AVP1 H+ -pump. Proceedings of the National Academy of Sciences of the United States of America 98, 11444–11449.

Halfter U, Ishitani M, Zhu JK. 2000. The Arabidopsis SOS2 protein kinase physically interacts with and is activated by the calcium-binding protein SOS3. Proceedings of the National Academy of Sciences of the United States of America 97, 3735-3740.

Kiegle E, Moore C, Haseloff J, Tester M, Knight M. 2000. Cell-type specific calcium responses to drought, NaCl, and cold in Arabidopsis root: a role for endodermis and pericycle in stress signal transduction. The Plant Journal 23, 267-278.

Krishna SS, Majumdar I, Grishin NV. 2003. Structural classification of zinc fingers: survey and summary. Nucleic Acids Research 31, 532-550.

Li N, Wang X, Ma B, Du C, Zheng L, Wang Y. 2017. Expression of a Na+/H+ antiporter RtNHX1 from a recretohalophyte Reaumuria trigyna improved salt tolerance of transgenic Arabidopsis thaliana Journal of Plant Physiology. In press. https://doi.org/10.1016/j.jplph.2017.07.015

Moore CA, Bowden HC, Scrase-Field S, Knight MR, White PJ. 2002. The deposition of suberin lamellae determines the magnitude of cytosolic Ca2+ elevations in root endodermal cells subjected to cooling. The Plant Journal 30, 457-65.

Munns R, Tester M. 2008. Mechanisms of salinity tolerance. The Annual Review of Plant Biology 59, 651- 681.

Munns R. 2005. Genes and salt tolerance: bringing them together. New Phytologist Journal 167, 645-663.

Paez-Valencia J, Patron-Soberano A, Rodriguez-Leviz A, Sanchez-Lares J, Sanchez-Gomez C, Valencia-Mayoral P, Diaz-Rosas G, Gaxiola R. 2011. Plasma membrane localization of the type I H+ -PPase AVP1 in sieve element-companion cell complexes from Arabidopsis thaliana. Plant Science 181, 23-30.

Quintero FJ, Ohta M, Shi H, Zhu JK, Pardo JM. 2002. Reconstitution in yeast of the Arabidopsis SOS signaling pathway for Na+ homeostasis. Proceedings of the National Academy of Sciences of the United States of America 99, 6061-9066.

Ravasi T, Huber T, Zavolan M, Forrest A, Gasterland T, Grimmond S, Hume DA. 2003. Systematic characterization of the zinc-finger-ontaining proteins in the mouse transcriptome. Genome Research 13, 1430-1442.

Seki M, Narusaka M, Ishida J, Nanjo T, Fujita M, Oono Y, Kamiya A, Nakajima M, Enju A, Sakurai T. 2002. Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. The Plant Journal 31, 279-292.

Shi H, Lee BH, Wu SJ, Zhu JK. 2002. Overexpression of a plasma membrane Na+/H+ antiporter gene improves salt tolerance in Arabidopsis thaliana. Nature Biotechnology 21, 81-85.

Sunarpi HT, Motoda J, Kubo M, Yang H, Yodo K. 2005. Enhanced salt tolerance mediated by AtHKT1 transporter-induced Na unloading from xylem vessels to xylem parenchyma cells. The Plant Journal 44, 928-938.

Taiz L, Zeiger E. 2010. Cellular and whole plant response to salinity stress. Plantphysiology. www.plantphys.net.

Tracy FE, Gilliham M, Dodd AN, Webb AAR, Tester M. 2008. NaCl-induced changes in cytosolic free Ca2+ in Arabidopsis thaliana are heterogeneous and modified by external ionic composition. Plant, Cell & Environment 31, 1063-1073.

Velasco R, Zharkikh A, Affourtit J, Dhingra A, Cestaro A, Kalyanaraman A, Fontana P, Bhatnagar SK, Troggio M, Pruss D, et al. 2010. The genome of the domesticated apple (Malus × domestica Borkh.) Nat Genet 2010: 42, 833- 839.

Zhang HX, Blumwald E. 2001. Transgenic salt-tolerant tomato plants accumulate salt in foliage but not in fruit. Nature Biotechnology 19, 765-768.

Zhu G, Spellman PT, Volpe T, Brown PO, Botstein D, Davis TN, Futcher B. 2000. Two yeast forkhead genes regulate the cell cycle and pseudohyphal growth. Nature 406, 90-94.