home icon user icon
Welcome to International Network for Natural Sciences | INNS Pub.
Home My Account
Submit Manuscript
double_slash
breadcumb_bg

In vivo study of the use of Thymus vulgaris essential oil in avian therapy

Paper Details

Research Paper 01/08/2018
Views (273) Download (6)
current_issue_feature_image
publication_file

In vivo study of the use of Thymus vulgaris essential oil in avian therapy

Merazi Yahya, Hammadi Kheira, Tou Abdenacer
Int. J. Biosci.13( 2), 200-211, August 2018.
Certificate: IJB 2018 [Generate Certificate]
Key: Antibiotic resistanceAromatherapyExtracts of medicinal plantsMicrobial agentsPoultrySensitivity

Abstract

An in-vivo therapeutic trial based on the essential oil, in order to solve the antibiotic resistance problem in ISA15 strain chickens. Escherichia coli choose for this study has a resistance to several antibiotics of veterinary interest. The study of antimicrobial activity by aromatogram of Thymus vulgaris oil, gave diameters of inhibitions between 13.33 and 35.33 with a large inhibitory effect. The LD50 calculate of EO is 4500mg/kg. The macrodilution method in a liquid medium made it possible to obtain a MIC of 5μl/mL with a bactericidal effect. Four groups were prepared (control); G II (oral receipt of 450mg/kg/day essential oil for five days); G III (intramuscularly received 0.1ml of an inoculum of 88.107CFU/ml of E. coli) G IV (received intramuscularly 0.1ml of an inoculum of 88.107CFU/ml of E. coli then treated with a dose of 450mg/kg/day orally for five days). The limits of the usual values ​​were calculated for the main biochemical and hematological parameters. The differences statistically assessed versus placebo, group GIII recorded a significant disturbance (P <0.05) for blood glucose, CRP, GB, ASAT and ALAT. While the GIV group has recorded a significant disruption for blood glucose, CRP, ASAT and creatinine. Group III recorded lesions of different organs (liver, air sacs, pericarditis) of an average (m ± esm) of 10.60 ± 0.50 larger than that of Group IV 3.60 ± 1.28, which statistically shows a significant difference between two samples t = 5.052 (p <0.05). Group III recorded 80% of deaths, double the IV group. No mortalities and lesions were reported to groups I and II. In this work, we have demonstrated the importance of the essential oil of T. vulgaris as an antimicrobial agent and as an alternative to improve some hematological and biochemical parameters in broiler chickens.

VIEWS 6
Cover PDF

Aafi A, Ghanmi M, Satrani B, Aberchane M, Ismaili My R, EL Abid A. 2011. Diversité et valorisation des principales plantes aromatiques et médicinales (PAM) de l’écosystème cédraie au Maroc. Centre de Recherche Forestière 16.

Allardyce R, Bienenstock J. 1984. Le système immunitaire au niveau des muqueuses chez l’homme sain et l’homme malade, plus particulièrement chez le sujet parasité. Bulletin of the World Health Organization 62, 367.

Berche P, Gaillard J, Simonet M. 1991. Les bactéries des infections humaines. Editeur Flammarion. Médecine et Sciences.

Berrada S, Bennani L, Chahbi A, Houssaini TS, Lalami AEO, Touimi GB, Houssaini FS. 2016. Effet antibactérien de deux huiles essentielles (Thymus vulgaris et Lavandula officinalis) sur des souches isolées d’un centre d’hémodialyse de la ville de Fès/[Antibacterial effect of two essential oils (Thymus vulgaris and Lavandula officinalis) on the isolated strains from Fez city’s hemodialysis center]. International Journal of Innovation and Applied Studies 17, 639.

Bolou G, Attioua B, N’guessan A, Coulibaly A, N’guessan J, Djaman A. 2011. Évaluation in vitro de l’activité antibactérienne des extraits de Terminalia glaucescens planch. sur Salmonella typhi et Salmonella typhimurium. Bulletin de la société royale des sciences de Liège.

Braun JP, Lefebvre H. 2008. Kidney function and damage. Clinical biochemistry of domestic animals 6, 485-528.

Clevenger J. 1928. Apparatus for the determination of volatile oil. Journal of Pharmaceutical Sciences 17, 345-349.

Diaby V, Yapo AF, Adon AM, Yapi HF, Djama AJ, Dosso M. 2016. Biotoxicité hématologique du sulfate de cadmium chez les rats Wistar. International Journal of Biological and Chemical Sciences 10, 1765-1772.

Domans H, Deans S. 2000. Antimicrobial agents from plants: antibacterial activity of plant volatiles oils. Journal of Applied Microbiology 88, 308-316.

Dorman H, Deans S. 2000. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of applied microbiology 88, 308-316.

El Ouali Lalami A, Fouad EA, Wissal O, Fouad OC, Rajae G, Hassane G. 2013. Composition chimique et activité antibactérienne des huiles essentielles de deux plantes aromatiques du centre nord marocain: Thymus vulagris et Thymus satureioïdis. Thymus vulagris 27-33.

Faugereml. 2015. Inflammation et schizophrénie: une étude électrophysiologique et psychométrique des liens entre protéine C-réactive, perception et qualité de vie, Aix-Marseille.

Gomes C, Lourenço ELB, Liuti ÉB, Duque AO, Nihi F, Lourenço AC, Mendes TC, Junior AG, Dalsenter PR. 2012. Evaluation of subchronic toxicity of the hydroethanolic extract of Tropaeolum majus in Wistar rats. Journal of ethnopharmacology 142, 481-487 DOI: 10.1016/j.jep.2012.05.023.

Goren E. 1978. Observations on experimental infection of chicks with Escherichia coli. Avian Pathology 7, 213-224 DOI: 10.1080/0307945780 8418274.

Griffin HD, Guo K, Windsor D, Butterwith SC. 1992. Adipose tissue lipogenesis and fat deposition in leaner broiler chickens. The Journal of nutrition 122, 363-368 DOI: 10.1093/jn/122.2.363.

Hammer KA, Carson CF, Riley TV. 1999. Antimicrobial activity of essential oils and other plant extracts. Journal of applied microbiology 86, 985-990.

Imbert A, Colombat M, Capron J. 2003. Démarche diagnostique devant une augmentation modérée et prolongée des transaminases. La Presse médicale 32, 73-78.

Jamroz D, Wertelecki T, Houszka M, Kamel C. 2006. Influence of diet type on the inclusion of plant origin active substances on morphological and histochemical characteristics of the stomach and jejunum walls in chicken. Journal of animal physiology and animal nutrition 90, 255-268 DOI: 10.1111/j.1439-0396.2005.00603.x.

Jeffrey J, Nolan LK, Tonooka K, Wolfe S, Giddings C, Horne S, Foley S, Lynne A, Ebert J, Elijah L. 2002. Virulence factors of Escherichia coli from cellulitis or colisepticemia lesions in chickens. Avian diseases 46, 48-52.

Jirovetz L, Buchbauer G, Stoyanova A, Metodiev S. 2000. Seasonal depending variations of the composition and biological activities of Douglas fir (Pseudotsuga menziesii) essential oils from Bulgaria. Scientia Pharmaceutica 68, 304-309.

Johnson J, Reid WM. 1970. Anticoccidial drugs: lesion scoring techniques in battery and floor-pen experiments with chickens. Experimental parasitology 28, 30-36.

Jorgensen JH. 2004. Who defines resistance? The clinical and economic impact of antimicrobial susceptibility testing breakpoints. Seminars in pediatric infectious diseases, Elsevier.

Kadir A, Touati AE. 2017. Etude de la sensibilité aux antibiotiques des souches de Staphylococcus aureus et d’entérobactéries isolées du lait camelin.

Koochaksaraie R, Irani M, Valizadeh M, Rahmani Z, Gharahveysi S. 2010. A study on the effect of cinnamon powder in diet on serum glucose level in broiler chicks. Global Veterinaria 4, 562-565.

Koynarski V, Mircheva T, Stoev S, Urumova V, Zapryanova D, Dishlyanova E, Koynarski T, Karov R. 2010. Pathoanatomical and blood biochemical investigations in chicks, challenged with Escherichia coli on the background of a pre-existing Eimeria infection. Rev. Med. Vet 161, 133-140.

Lezzar N, Benmakhlouf A. 2006. Influence d’un traitement oral à la Fluméquine sur la résistance aux quinolones des souches d’Escherichia coli dans la flore fécale du poulet de chair, Université Mentouri de Constantine.

Mahangaiko M. 2016. Effets de la perfusion intensive sur quelques paramètres sanguins chez des veaux atteints de diarrhée aiguë. Journal of Applied Biosciences 104, 9942-9946.

Martin V. 2010. Les processus inflammatoires chez les oiseaux: physiopathologie et implications cliniques en aviculture.

Merazi Y, Hammadi K. 2018. In vitro study of the activity of essential oils of Thymus capitatus and Thymus vulgaris against enterobacteria of avian origin resistant to antibiotics. International Journal of Biosciences 12, 292-307 DOI: http://dx.doi.org/ 10.12692/ ijb/12.6.292-307.

Merazi Y, Hammadi K, Fedoul FF. 2016. Approche Ethno-Vétérinaire Des Plantes Médicinales Utilisées Dans La Région De Sidi Bel Abbes-Algérie. European Scientific Journal 12.

Mith H, Dure R, Delcenserie V, Zhiri A, Daube G, Clinquart A. 2014. Antimicrobial activities of commercial essential oils and their components against food‐borne pathogens and food spoilage bacteria. Food science & nutrition 2, 403-416.

Mogenet L, Bezille P, Guyonnet J, Karembe H. 1997. Comparaison de la flumequine (flumisol) a l’amoxicilline (vetrimoxin poudre orale) dans deux modes d’administration par voie orale, en traitement de la colibacillose du poulet: approche pharmacodynamique et clinique. Revue de médecine vétérinaire 148, 793-804.

Nakamura K, Imada Y, Abe F. 1987. Effect of cyclophosphamide on infections produced by Escherichia coli of high and low virulence in chickens. Avian Pathology 16, 237-252 DOI: 10.1080/0307945 8708436372.

NCCLS. 1997. Performance standards for antimicrobial disk susceptibility tests :Approved standard M2-A7. National Committee for Clinical Laboratory Standards, Wayne, PA, USA.

Nwaoguikpe R. 2010. Plasma glucose, protein and cholesterol levels of chicks or birds maintained on pawpaw (Carica papaya) seed containing diet. Pakistan Journal of Nutrition 9, 654-658.

OECD. 2001. Organization for Economic Co-operation and Development. Test n°423: Acute oral toxicity – Acute toxic class method. Organisation for Economic Co-operation and Development, Paris.

Pascalon‐Pekelniczky A, Michoudet C, Chauve CM. 1996. Modifications enzymatiques sanguines chez la canette mularde (Catrina moschata × Anas platyrhynchos) lors d’infection expérimentale par Eimeria mulardi. Avian pathology 25, 785-798.

Rahmatallah N, Nassik S, El Rhaffouli H, Lahlou Amine I, El Houadfi M. 2016. Détection de souches multi-résistantes d’Escherichia coli d’origine aviaire dans la région de Rabat Salé Zemmour Zaer. Revue Marocaine des Sciences Agronomiques et Vétérinaires 5.

Rajman M, Juráni M, Lamošová D, Máčajová M, Sedlačková M, Košťál Ľ, Ježová D, Výboh P. 2006. The effects of feed restriction on plasma biochemistry in growing meat type chickens (Gallus gallus). Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 145, 363-371 DOI: 10.1016/j.cbpa.2006.07.004.

Sangwan N, Farooqi A, Shabih F, Sangwan R. 2001. Regulation of essential oil production in plants. Plant growth regulation 34, 3-21.

Satrani B, Ghanmi M, Farah A, Aafi A, Fougrach H, Bourkhiss B, Bousta D, Talbi M. 2007. Composition chimique et activité antimicrobienne de l’huile essentielle de Cladanthus mixtus. Bull Soc Pharm Bordeaux 146, 85-96.

Soković M, Glamočlija J, Marin PD, Brkić D, van Griensven LJ. 2010. Antibacterial effects of the essential oils of commonly consumed medicinal herbs using an in vitro model. Molecules 15, 7532-7546.

Stordeur P, Mainil J. 2002. La colibacillose aviaire. Annales de médecine vétérinaire, Annales Medecine Veterinaire.

Tenaillon O, Skurnik D, Picard B, Denamur E. 2010. The population genetics of commensal Escherichia coli. Nature Reviews Microbiology 8, 207.

Tremblay BL. 2015. Effet des polymorphismes des gènes des phospholipases A2 sur la variabilité interindividuelle des facteurs de risque cardiométaboliques suite à une supplémentation en acides gras oméga-3 d’origine marine.

Van Vuuren S, Suliman S, Viljoen A. 2009. The antimicrobial activity of four commercial essential oils in combination with conventional antimicrobials. Letters in applied microbiology 48, 440-446.

Viau C, Tardif R. 2003. Toxicologie. Environnement et santé publique: Fondements et pratiques 143, 120-123.

Vinodini N, Chatterjee PK, Chatterjee P, Chakraborti S, Nayanatara A, Bhat RM, Rashmi K, Suman V, Shetty SB, Pai SR. 2015. Protective role of aqueous leaf extract of Moringa oleiferaon blood parameters in cadmium exposed adult wistar albino rats. International Journal of Current Research & Academic Review 3, 192-199.

Wyss M, Kaddurah-Daouk R. 2000. Creatine and creatinine metabolism. Physiological reviews 80, 1107-1213 DOI: 10.1152/physrev.2000.80.3.1107.

Yeh ET, Willerson JT. 2003. Coming of age of C-reactive protein: using inflammation markers in cardiology. Circulation 107, 370-371.

Yogaratnam V. 1995. Analysis of the causes of high rates of carcase rejection at a poultry processing plant. The Veterinary Record 137, 215-217.

Submit Manuscript