Influence of casein and inulin on the properties of nano-particle encapsulation of fish oil

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Research Paper 01/02/2014
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Influence of casein and inulin on the properties of nano-particle encapsulation of fish oil

Maryam Saberi, Mahnaz Hashemiravan, Nazanin Farhadyar
J. Bio. Env. Sci.4( 2), 318-326, February 2014.
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The purpose of this study was to investigate the influence of two components Casein and Inulin with known functional properties as wall material on properties of fish oil encapsulated powders. Two different level of concentration of fish oil and five different levels of combination Casein and Inulin were studied. Ultrasonic have been used for nano-emulsion preparation. The size and the pH of nano-emulsion were characterized. Results showed that, while both wall materials were capable of producing nano-emulsion of the size range of 97.4-148.433 nm, the Casein was the best wall material. The feed mixtures were dried in a mini spray dryer. Particle structure of fish oil encapsulated powder were evaluated by scanning electron microscope. The encapsulation efficiency, the moisture and the surface content of fish oil encapsulated powder were determined. The results revealed that sample containing 20%wt Casein and 8%wt fish oil were the best sample to achieve minimum amount of surface oil(31.67mg/100 g powder), maximum encapsulation efficiency (86.566%) and minimum moisture content(0.711).


Cho YH, Shim HK, Park J. 2003. Encapsulation of fish oil by an enzymatic gelation process using transglutaminase cross-linked proteins. Journal Food Science 68, 2717-2723.

Chung C, Sanguansri L, Augustin MN. 2010. In vitro lipolysis fish oil microcapsules containing protein and resistant starch. Food Chemistry.

eD Vos P, Faas M, Spasojevic M, Sikkema J. 2010. Encapsulation for preservation of functionality and targeted delivery of bioactive food components. International Dairy Journal 20, 292 – 302.

Diaz-Rojas EI, Pacheco-Aguilar R, Lizardi J, Arguelles-Morales W, Valdez MA, Rinaudo M, Goycoolea FM. 2004. Linseed pectin: Gelling properties and performance as an encapsulation matrix for shark liver oil. Food Hydrocolloid 18, 293-304.

Jafari SM, He Y, Bhandari B. 2007a. Production of sub-micron emulsions by ultrasound and microfluidization techniques. Journal of Food Engineering 82, 478–488.

Jafari SM, He Y, Bhandari B. 2007b. Effectiveness of encapsulating biopolymers to produce sub-micron emulsions by high energy emulsification techniques. Food Research International 40, 862–873.

Jafari SM, Assadpoor E, Bhandari B, He Y. 2008. Nano-particle encapsulation of fish oil by spray drying. Food Research International 41, 172–183.

Kolanowski W, Ziolkowski M, WeiBbordt J, Kunz B, Laufenberg G. 2006. Microencapsulation of fish oil by spray-dring on oxidative stability. Part 1.Eurpean Food Resource Technology 222, 336–342.

Lin C, Lin S, Hwang L. 1995. Microencapsulation of Squid oil with hydrophilic macromolecules for oxidative and thermal stabilization. Journal of Food Science Nutrient 55, 333-343.

Pinto Reis C, Neafeld RJ. 2006. Nnoencapsulation. Methods for Preparation of drug loaded polymeric nanoparticle Nunomedicine 2, 8-21.

Quintanilla-Carvajal MX, Camacho-Diaz BH, Meraz-Torres LS, Chanona-Perez JJ, Alamilla-Beltran L. 2010. Nanoencapsulation: A New Trend in Food Engineering Processing. Food Engineering Review 2, 39 – 50.

Rosenberg. Milk drived whey protein – based micro encapsulation agents and method of use, u.s patents No. 560-1760.

Rubio-Rodriguz N, Beltran S, Jaime I, Diego SMDE, Sanz MT, Carballido JR. 2010. Production of omega-3 polyunsaturated fatty acid concentrates: A: review.Innovative food science and Technologies 11, 1-12.

Semo E, Kesselman E, Danino D, Livney YD. 2007. Casein micelle as a natural nano-capsular vehicle for nutraceuticals. Food Hydrocolloids 21, 936–942.

Sukhorukova G, Feryb A, Mohwald H.2007.Intelligent micro and nanocapsules Science 30, 885-897.

Summer A, Formuggioni P, Franceschi P, malacarne M. 2003. Proteose-Peptone content the milk of Italian Fresiuen Cows with moderate and light Somatic cell values. Ann. Fac. Medic 21, 169-173.

Yue X, xu z, pringawiwa tkul JN, losso JM, Godber JS. 2007. Gomparision of soybean oils, gum and defatted soy flour extraction stabilizing menhaden oil during heating. Journal of food chemistry.