Local plants as sources of the phytoecdysteroid, 20-hydroxyecdysone
Paper Details
Local plants as sources of the phytoecdysteroid, 20-hydroxyecdysone
Abstract
Ecdysteroids derived their name from the insect molting process known as ecdysis. They are substances extensively explored as growth promoters for both plants and insects, essentially harmless to humans and appear to have a number of side effects that are considered to be beneficial. Physiologically active plant steroids known as phytoecdysteroids (PEs) contain structures like those of insect molting hormones are widely distributed in plants and acting as defense against phytophagous (plant-eating) insects. Numerous biological, pharmacological, and therapeutic features of PEs have been linked to their use in the treatment and prevention of both acute and chronic illnesses. The most abundant and common PE is the 20-hydroxyecdysone (20E). Achyranthes aspera L., Amaranthus spinosus L., Ipomea pes-caprae L. and Portulaca oleracea L are common local plants in the Philippines and were investigated to determine the presence of 20E through High Performance Liquid Chromatography. Achyranthes aspera L., Amaranthus spinosus L., and Portulaca oleracea L. contained 2 peaks close to the retention time of the target compound. Ipomea pes-caprae L., only one peak is observed within ± 0.120 minutes. Plant samples, Ipomea pes-caprae L. and Portulaca oleracea L. had peaks with retention times not significantly different from that of the standard were found, indicating presence of 20-hydroxyecdysone in these samples. The ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS) to confirm the presence or absence of 20E can be done as well to identify the other components of the samples, particularly those eluting near the retention time of 20E.
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