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Motivate the production of pharmaceutical compounds in Ocimum basilicum by magnetic phosphorus solution and Arbuscular mycorrhizal fungi

Research Paper | September 1, 2017

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Edris Shabani, Sahebali Bolandnazar, Seyed Jalal Tabatabaei, Nosratollah Najafi, Saeedeh Alizadeh-Salteh

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J. Bio. Env. Sci.11( 3), 31-45, September 2017


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Vegetable production by using of physical methods as a way to increase the quantity and quality of crops are considered. A greenhouse experiment was conducted to assess the effects of magnetic field (G), arbuscular mycorrhizal fungi (M) and phosphorus (P) concentration in the nutrient solution (0, 5, 10, 20 and 40 mg L-1) on the fresh weight, antioxidant enzymes activity, production of phenolic compounds and essential oil components of basil plants. The experiment was designed as a factorial combination and treatments were arranged in a completely randomized design with four replicates. Treatment of basil plants with G, M and P led to increase of the plant growth, carotenoid contents (CAR), antioxidant activity, antioxidant enzyme contents such as catalase (CAT) and proxidase (POD) as well as decrease in phenol and flavonoid compounds content. Methyl chavicol, methyl eugenol and sesquiterpenes like α–bisabolene, α-humulene and caryophyllene content was increased by magnetic P solution and mycorrhizal fungi colonization. Therefore, magnetic P solution and M potentially represent natural ways of promoting growth and motivate the production of pharmaceutical compounds in this important medicinal herb.


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Motivate the production of pharmaceutical compounds in Ocimum basilicum by magnetic phosphorus solution and Arbuscular mycorrhizal fungi

Aebi H. 1984. Catalase in vitro. Methods in enzymology 105, 121-126. https://doi.org/10.1016/S0076-6879(84).05016-3

Ahmed Ibrahim M. 2013. Effects of magnetized low quality water on some soil properties and plant growth. International Journal of Research in Chemistry and Environment 3, 140-147.

Akhtar M, Oki Y, Adachi T. 2009. Mobilization and acquisition of sparingly soluble P-sources by Brassica cultivars under P-starved environment II. Rhizospheric pH changes, redesigned root architecture and Pi-uptake kinetics. Journal of Integrative Plant Biology 51, 1024–1039. https://doi.org/10.1111/j.1744-7909.2009.00874.x

Aladjadjiyan A. 2007. The use of physical methods for plant growing stimulation in Bulgaria. Journal of Central European Agriculture 8, 369-380.

Aliverdi AM, Parsa M, Hammami H. 2015. Increased soybean-rhizobium symbiosis by magnetically treated water. . Biological Agriculture and Horticulture 31, 167-176. https://doi.org/10.1080/01448765.2014.996253

Astani A, Reichling J, Schnitzler P. 2011. Screening for antiviral activities of isolated compounds from essential oils. Evidence-based complementary and alternative medicine 1-8. https://doi.org/10.1093/ecam/nep187

Bolandnazar S, Aliasgarzad N, Neishabury MR, Chaparzadeh N. 2007. Mycorrhizal colonization improves onion (Allium cepa L.) yield and water use efficiency under water deficit condition. Scientia horticulturae 114, 11-15. https://doi.org/10.1016/j.scienta.2007.05012

Bradford MB. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254. https://doi.org/10.1016/0003-2697(76)9052.7-3

Bryant JP, Chapin FS, Klein DR. 1983. Carbon/nutrient balance of boreal plants in relation to vertebrate herbivory. Oikos 357-368. https://doi.org/10.2307/3544308

Cantin CM, Moreno MA, Gogorcena Y. 2009. Evaluation of the antioxidant capacity, phenolic compounds and vitamin C content of different peach and nectarine (Prunus persica (L.) Batsch) Breeding Progenies. Journal of Agricultural and Food Chemistry 57, 4586–4592. https://doi.org/10.1021/jf900385a

Chance B, Maehly AC. 1955. Assay of catalases and peroxidases. Methods in Enzymology 2, 764-775. https://doi.org/10.1016/S0076-6879(55)023.00-8

Chang CC, Yang MH, Wen HM, Chern JC. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. . Journal of Food and Drug Analysis 10,178-182.

Chang KT, Weng CI. 2006. The effect of an external magnetic field on the structure of liquid water using molecular dynamics simulation. Journal of Applied Physics 100, 1–6. http://dx.doi.org/10.1063/1.2335.971

Colla G, Rouphael Y, Di Mattia E, El-Nakhel C, Cardarelli M. 2015. Co-inoculation of Glomus intraradices and Trichoderma atroviride acts as a biostimulant to promote growth, yield and nutrient uptake of vegetable crops. Journal of the Science of Food and Agriculture 95, 1706-1715. https://doi.org/10.1002/jsfa.6875

Copetta A , Lingua G,  Berta G. 2006. Effects of three AM fungi on growth, distribution of glandular hairs, and essential oil production in Ocimum basilicum L. var. Genovese.  Mycorrhiza 16, 485–494. https://doi.org/10.1007/s00572-006-0065-6

Dalia AS, Gendy SA, Maria AM, Mousa EM. 2009. Response of pepper plants (Capsicum annuum L.) to magnetic technologies. In: Proceedings of the first Nile Delta Conference on Export Crops.

Dat JF, Van Montagu M, Inze D, Van Breusegem F. 2001. Catalase deficient tobacco plants: Tools for in plant studies on the role of hydrogen peroxide. Redox Report 6, 37–42. http://dx.doi.org/10.1179/1351000011015.36012

Devi MC, Reddy MN. 2002. Phenolic acid metabolism of groundnut (Arachis hypogaea L.) plants inoculated with VAM fungus and Rhizobium. Plant Growth Regulation., 37(2), 151-156.

Dong X, Tang B, Li J, Xu Q, Fang S,  Hua Z. 2008. Expression and purification of intact and functional soybean (Glycine max) seed ferritin complex in Escherichia coli. Journal of Microbiology and Biotechnology 18, 299–307.

Gang DR, Wang J, Dudareva N, Hee NK, Simon JE. 2001. An investigation of the storage and biosynthesis of phenylpropanes in sweet basil. Plant Physiology 125, 539–555. https://doi.org/10.1104/pp.125.2.539

Garcia-Reina F,  Pascual LA. 2001. Influence of a stationary magnetic field on water relations in lettuce seeds. Part I: Theoretical considerations. Bioelectromagnetism 22, 589–595. https://doi.org/10.1002/bem.88

Ghanati F,  Abdolmaleki P, Vaezzadeh M, Rajabbeigi E, Yazdani M. 2007. Application of magnetic field and iron in order to change medicinal products of Ocimum basilicum. Environmentalist 27, 429–434. https://doi.org/10.1007/s10669-007-9079-7

Ghanati F, Mohamadalikhani S, Soleimani M, Afzalzadeh R Hajnorouzi A. 2015. Change of growth pattern, metabolism, and quality and quantity of maize plants after irrigation with magnetically treated water. Electromagnetic Biology and Medicine 34, 211-215. http://dx.doi.org/10.3109/15368378.2015.1076453

Grewal HS, Maheshwari BL. 2011. Magnetic treatment of irrigation water and snow pea and chickpea seeds enhances early growth and nutrient contents of seedlings. Bioelectromagnetism 32, 58–65. https://doi.org/10.1002/bem.20615

Hajnorouzi A, Vaezzadeh M, Ghanati F, jamnezhad H, Nahidian B. 2011. Growth promotion and a decrease of oxidative stress in maize seedlings by a combination of geomagnetic and weak electromagnetic fields. Journal of Plant Physiology 168, 1123-1128. https://doi.org/10.1016/j.jplph.2010.12.003

Hassanpouraghdam MB, Gohari GR, Tabatabaei SJ, Dadpour MR. 2010. Inflorescence and leaves essential oil composition of hydroponically grown Ocimum basilicum L. Journal of the Serbian Chemical Society 75, 1361–1368. https://doi.org/10.2298/JSC10031111.3H

Hassanpouraghdam, MB, Gohari GR, Tabatabaei, SJ, Dadpour MR, Shirdel M. 2011. NaCl salinity and Zn foliar application influence essential oil composition of basil (Ocimum basilicum L.). Acta agriculturae Slovenica 97, 93-98. https://doi.org/10.2478/v10014-011-0004-x

Javanmardi J, Khalighi A, Kashi A, Bais HP, Vivanco JM. 2002. Chemical characterization of basil (Ocimum basilicum L.) found in local accessions and used in traditional medicines in Iran. Journal of Agriculture Food Chemistry 50, 5878–588. https://doi.org/10.1021/jf020487q

Javanmardi J, Stushnoff C, Locke E, Vivanco J.M. 2003. Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chemistry 83, 547-550. https://doi.org/10.1016/S0308-8146(03)00.151-1

Jokubauskaite I, Karcauskiene D, Antanaitis S, Mazvila J, Slepetiene A, Koncius D, Piaulokaite-Motuziene L. 2015. The distribution of phosphorus forms and fractions in Retisol under different soil liming management. Zemdirbyste (Agriculture) 102, 251-256. https://doi.org/10.13080/z-a.2015.102.032

Koleva II, van Beek TA, Linssen JPH, de Groot A, Evstatieva LN. 2002. Screening of plant extracts for antioxidant activity: A comparative study on three testing methods. Phytochemical Analysis 13, 8-17. https://doi.org/10.1002/pca.611

Larson R. 1988. The antioxidants of higher plants. Phytochemistry 27, 969–978. https://doi.org/10.1016/0031-9422(88)802.54-1

Lichtenthaler, HK, Buschmann C. 2001. Chlorophylls and carotenoids: Measurement and characterization by UV‐VIS spectroscopy. Current protocols in food analytical chemistry. https://doi.org/10.1002/0471142913.faf0403s01

Miransari M. 2010. Contribution of arbuscular mycorrhizal symbiosis to plant growth under different types of soil stress. Plant Biology 12, 563–569. https://doi.org/10.1111/j.1438-8677.2009.00308.x

Mollavali M, Bolandnazar SA, Schwarz D, Rohn S, Riehle P, Zaare Nahandi F. 2016. Flavonol Glucoside and Antioxidant Enzyme Biosynthesis Affected by Mycorrhizal Fungi in Various Cultivars of Onion (Allium cepa L.). Journal of Agriculture Food Chemistry 64, 71-77. https://doi.org/10.1021/acs.jafc.5b04791

Nguyen PM, Niemeyer ED. 2008. Effects of nitrogen fertilization on the phenolic composition and antioxidant properties of basil (Ocimum basilicum L.). Journal of Agriculture Food Chemistry 56, 8685-8691. https://doi.org/10.1021/jf801485u

Park KR, Nam D, Yun HM, Lee SG, Jang HJ, Sethi G, Cho SK, Ahn KS. 2011. β-Caryophyllene oxide inhibits growth and induces apoptosis through the suppression of PI3K/AKT/mTOR/S6K1 pathways and ROS-mediated MAPKs activation. Cancer letters 312, 178-188. https://doi.org/10.1016/j.canlet.2011.08.0.01

Rojas-Andrade R, Cerda-García-Rojas C, Frías-Hernández J, Dendooven L, Olalde-Portugal V, Ramos-Valdivia A. 2003. Changes in the concentration of trigonelline in a semi-arid leguminous plant (Prosopis laevigata) induced by an arbuscular mycorrhizal fungus during the presymbiotic phase. Mycorrhiza., 13(1), 49-52.

Sadeghipour O, Aghaei P. 2013. Improving the growth of cowpea (Vigna unguiculata L. Walp.) by magnetized water. Journal of Biodiversity and Environmental Science 3, 37–43.

Sahebjamei H, Abdolmaleki P, Ghanati F. 2007. Effects of magnetic field on the antioxidant enzyme activities of suspension‐cultured tobacco cells. Bioelectromagnetics 28(1), 42-47. https://doi.org/10.1002/bem.20262

Sinha S, Saxena R. 2006. Effect of iron on lipid peroxidation, and enzymatic and non-enzymatic antioxidants and bacoside-A content in medicinal plant Bacopa monnieri L. Chemosphere 62, 1340–1350. https://doi.org/10.1016/j.chemosphere.2005.07.03.0

Soltani F, Kashi A, Arghavani M. 2006. Effect of magnetic field on Asparagus originalis L. Seed germinaton and seedling growth. Seed Science and Technology 34, 349-353. https://doi.org/10.15258/sst.2006.34.210

Toledo EJL, Ramalho TC, Magriotis ZM. 2008. Influence of magnetic field on physical–chemical properties of the liquid water:Insights from experimental and theoretical models. Journal of Molecular Structure 888, 409–425. https://doi.org/10.1016/j.molstruc.2008.01.0.10

Toussaint  JP, Smith FA, Smith SE. 2007. Arbuscular mycorrhizal fungi can induce the production of phytochemicals in sweet basil irrespective of phosphorus nutrition. Mycorrhiza 17, 291–297. https://doi.org/10.1007/s00572-006-01.04-3

Turker M, Temirci C, Battal P, Erez ME. 2007. The effects of an artificial and static magnetic field on plant growth, chlorophyll and phytohormone levels in maize and sunflower plants. Phyton Annales Rei Botanicae 46, 271–284.

Velioglu YS, Mazza G, Gao L, Oomah BD. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of Agricultural Food and Chemistry 46, 4113–4117. https://doi.org/10.1021/jf9801973

Waterhouse AL. 2001. Determination of total phenolics. Current protocols in food analytical chemistry. John Wiley and Sons Inc, New York. https://doi.org/10.1002/0471142913.faa0101s06

Yao MK, Desilets H, Charles MT, Boulanger R, Tweddell RJ. 2003. Effect of mycorrhization on the accumulation of rishitin and solavetivone in potato plantlets challenged with Rhizoctonia solani. Mycorrhiza. 13(6), 333-336.

Zaks A, Klibanov AM. 1988. The effect of water on enzyme action in organic media. The Journal of Biological Chemistry 263, 8017–8021.

Zhang J,  Zhou K,  Wang L, Gao M. 2014. Extremely low‑frequency magnetic fields affect pigment production of Monascus purpureus in liquid‑state fermentation. European Food Research and Technology 238, 157–162. https://doi.org/10.1007/s00217-013-2096-5