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Optimization of various parameters for selenium enriched yeast production

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Research Paper 01/08/2020
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Optimization of various parameters for selenium enriched yeast production

Fatima Gul, Bashir Ahmad, Sarzamin Khan
Int. J. Biosci.17( 2), 218-224, August 2020.
Certificate: IJB 2020 [Generate Certificate]
Key: FeedParametersSaccharomyces cerevisiaeSe enriched yeastSelenium

Abstract

Selenium is an essential trace element for humans, animals and poultry. It belongs to body’s antioxidant defense system. There are two major sources of selenium i.e. organic and inorganic. Selenium enriched yeast is the finest source of organic selenium. Yeast production can easily be achieved in laboratory as it utilizes soluble sugars and organic acids to yield biomass with high protein content. In this study the experiments were designed for optimizing culture conditions for the production of selenium enriched yeast. The statistical analysis of the data was performed on statistical software JMP predicted variables for optimization. The data of variables covered a wide range of values for fermentation temperature, shaking speed, pH, incubation time, Selenium concentration and selenium adding time to built-in response for variables to specific yeast biomass. Morphological characteristics such as colonial morphology (colony shape, colour, and surface appearance) and yeast biochemical identification on metabolized glucose sucrose, lactose, cellibiose express genes that activate the synthesis of yeast. The optimized values for Temp 25oC, pH 4, Se conc. 30 µg/ml, addition of Se after 9 hr of incubation, shaking speed 130rpm and incubation time 48 hrs yielded total biomass (65.59g/L) and selenium accumulation (46.23mg/kg) respectively. It was concluded that using a culture medium supplemented with 30 μg/mL sodium selenite identified the optimum fermentation conditions initial sodium selenite (Na2SeO3) concentration, initial pH and temperature) from Saccharomyces cerevisiae for maximal total Se yield of selenium-enriched yeast.

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Aggarwal A, Upadhyay R. 2013. Heat stress and immune function. In: Heat stress and animal productivity: Springer p. 113-136.

Alidee T, Habbal H, Tohla M. 2016. Optimization of selenium enriched Saccharomyces cerevisiae by Response Surface Methodology (RSM). International Journal of ChemTech Research 9, 221-226.

Amirimm, Fazeli MR, Amini M, Roodbari NH, Samadi N. 2017. Optimization of Culture Conditions for Enrichment of Saccharomyces cerevisiae with Dl-α-Tocopherol by Response Surface Methodology. Iranian Journal of Pharmaceutical Research: IJPR 16, 1546.

Avery JC, Hoffmann PR. 2018. Selenium, selenoproteins, and immunity. Nutrients 10, 1203.

Briens M, Mercier Y, Rouffineau F, Vacchina V, Geraert PA. 2013. Comparative study of a new organic selenium source v. seleno-yeast and mineral selenium sources on muscle selenium enrichment and selenium digestibility in broiler chickens. British Journal of Nutrition 110, 617-624.

Diarra SS, Tabuaciri P. 2014. Feeding management of poultry in high environmental temperatures. International journal of poultry science 13, 657-661.

Esmaeili S, Khosravi-Darani K, Pourahmad R, Komeili R. 2012. An experimental design for production of selenium-enriched yeast. World Appl Sci 19, 31-37.

Gupta M, Gupta S. 2017. An overview of selenium uptake, metabolism, and toxicity in plants. Frontiers in Plant Science 7, 2074.

Krinsky NI. 2001. Carotenoid antioxidants. Journal of Nutrition, 17, 815-7.

Khan FA. 2017. Replacement of protein source in the existing poultry feed with single cell microbial protein. In: University of Peshawar.

Kieliszek M, Błażejak S, Bzducha-Wrobel A, Kot AM. 2019. Effect of selenium on growth and antioxidative system of yeast cells. Molecular biology reports 46, 1797-1808.

Kurek E, Ruszczynska A, Wojciechowski M, Luciuk A, Michalska-Kacymirow ME. 2016. Bio-transformation of selenium in Se-enriched bacterial strains of Lactobacillus casei. Roczniki Państwowego Zakładu Higieny 67.

Kurtzman C, Fell JW, Boekhout T. 2011. The yeasts: a taxonomic study: Elsevier.

McDonald V. 1963. Direct microscopic technique to detect viable yeast cells in pasteurized orange drink. Journal of Food Science 28, 135-139.

Michalska-Kacymirow M, Kurek E, Smolis A, Wierzbicka M, Bulska E. 2014. Biological and chemical investigation of Allium cepa L. response to selenium inorganic compounds. Analytical and bioanalytical chemistry 406, 3717-3722.

Nasir A, Rahman SS, Hossainmm, Choudhury N. 2017. Isolation of Saccharomyces cerevisiae from pineapple and orange and study of metal’s effectiveness on ethanol production. European Journal of Microbiology and Immunology 7, 76-91.

Ogawa Y, Nitta A, Uchiyama H, Imamura T, Shimoi HI, to K. 2000. Tolerance mechanism of the ethanoltolerant mutant of sake yeast. Journal of Bioscience and Bioengineering. 90, 313-20

Oliveira VA, Vicente MA, de Oliveira Santos J, Araújo LD. 2008. Biochemical and molecular characterization of Saccharomyces cerevisiae strains obtained from sugar-cane juice fermentations and their impact in cachaça production. Appl. Environ. Microbiol 74, 693-701.

Orabymm, Allababidy T, Ramadan E. 2015. The bioavailability of selenium in Saccharomyces cerevisiae. Annals of Agricultural Sciences 60, 307-315.

Pena A, Sanchez NS, Alvarez H, Calahorra M, Ramírez J. 2015. Effects of high medium pH on growth, metabolism and transport in Saccharomyces cerevisiae. FEMS yeast research 15.

Salari R, Salari R. 2017. Investigation of the best Saccharomyces cerevisiae growth condition. Electronic physician 9, 3592.

Suhajda A, Hegoczki J, Janzso B, Pais I, Vereczkey G. 2000. Preparation of selenium yeasts I. Preparation of selenium-enriched Saccharomyces cerevisiae. Journal of Trace Elements in Medicine and Biology 14, 43-47.

Surai P. 2002. Selenium in poultry nutrition, Antioxidant properties, deficiency and toxicity. World’s poultry science journal 58, 333-347.

Yun CW, Bauler M, Moore RE, Klebba PE, Philpott CC. 2001. The role of the FRE family of plasma membrane reductases in the uptake of siderophore iron in Saccharomyces cerevisiae. Journal of Biol.Chem. 276, 10218-23.

Zare H, Owlia P, Vahidi H, Khujin MH. 2018. Simultaneous Optimization of the Production of Organic Selenium and Cell Biomass in Saccharomyces Cerevisiae by Plackett-Burman and Box-Behnken Design. Iranian Journal Of Pharmaceutical Research: IJPR 17, 1081.

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