PERSISTENCE OF LOCAL STRAIN OF ENTOMOPATHOGENIC FUNGI ASPERGILLUS FLAVUS IN INDOOR AIR

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Research Paper 01/03/2019
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PERSISTENCE OF LOCAL STRAIN OF ENTOMOPATHOGENIC FUNGI ASPERGILLUS FLAVUS IN INDOOR AIR

Amina Abrar, Tahira Aziz Mughal, Muhammad Oneeb, Eza Tahir, Salma Khalid, Kausar Malik
Int. J. Biosci.14( 3), 133-149, March 2019.
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Abstract

To assess the persistence of entomopathogenic fungi Aspergillus flavus in indoor air, its local strain (FCBP-PTF-003) was isolated from Changa Manga forest near Lahore. Aspergillus flavus is considered pathogenic to insects but also have record of being dangerous to other organism and plants. Before using this fungus against insects, to assess its risk as potential human allergens, airborne conidia were measured in enclosed environments. To evaluate its persistence in indoor air, a spray formulation of Aspergillus flavus conidial solution was applied in a room selected for the experiment. Air sampling was carried out for three consecutive days with an interval of twenty four hours. Conidial count of CFU/m3 obtained on media plates was recrded after five days of incubation. The conidial count of three consecutive days was recorded as 100,801, 57,940 and 3,184 CFU/m3. Total viable airborne fungal counts varied as it reduced successively after three days. It was evident from the analysis that conidia of Aspergillus flavus do persist in air however the significant reduction in CFU/m3 after time indicate that its persistence is not for the longer period of time. The local strain is found to be not much dangerous compared to the notorious reputation of Aspergillus flavus.

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Alves SB, Alves L A, Lopes RB, Pereira RM, Vieira SA. 2002. Potential of some Metarhizium anisopliae isolates for control of Culex quinquefasciatus (Dipt. Culicidae). Journal of Applied Entomology 26, 504–509. https://doi.org/10.1046/j.1439-0418.2002.00674.x 

Araujo JPM, Hughes DP. 2016. Diversity of entomopathogenic fungi: which groups conquered the insect body? Advances in Genetics 94, 1-39. https://doi.org/10.1016/bs.adgen.2016.01.001

Chandler D, Hay D, Reid AP. 1997. Sampling and occurrence of entomopathogenic fungi and nematodes in UK soils. Applied Soil Ecology 5(2), 133-141. https://doi.org/10.1016/s0929-1393(96)00144-8 

Darbro JM, Thomas MB. 2009. Spore persistence and likelihood of aero allergenicity of entomopathogenic fungi used for mosquito control. The American journal of tropical medicine and hygiene 80(6), 992-997. https://doi.org/10.4269/ajtmh.2009.80.992 

Drummond J, Pinnock DE. 1990. Aflatoxin production by entomopathogenic isolates of Aspergillus parasiticus and Aspergillus flavus. Journal of invertebrate pathology 55(3), 332-336. https://doi.org/10.1016/0022-2011(90)90075-h

Hedayati MT, Pasqualotto AC, Warn, PA, Bowyer P, Denning DW. 2007.  Aspergillus flavus: human pathogen, allergen and mycotoxin producer. Microbiology 153(6), 1677-1692. https://doi.org/10.1099/mic.0.2007/007641-0 

Inglis GD, Goettel MS, Butt TM, Strasser H. 2001. Use of hyphomycetous fungi for managing insect pests. Mississippi. CABI Publishing, p 23-69. https://doi.org/10.1079/9780851993560.0023

Meyling NV, Eilenberg J. 2006. Occurrence and distribution of soil borne entomopathogenic fungi within a single organic agro ecosystem. Agriculture Ecosystem and Environment 113(1), 336-341. https://doi.org/10.1016/j.agee.2005.10.011

Reddy MK, Srinivas T. 2017. Mold Allergens in Indoor Play School Environment. Energy Procedia 109, 27-33. https://doi.org/10.1016/j.egypro.2017.03.042

Rosas I, Calderón C, Martínez L, Ulloa M, Lacey J. 1997. Indoor and outdoor airborne fungal propagule concentrations in Mexico City. Aerobiologia 13(1), 23-30. https://doi.org/10.1007/bf02694787 

Singh B, Singh S, Asif AR, Oellerich M, Sharma GL. 2014. Allergic aspergillosis and the antigens of Aspergillus fumigatus. Current Protein and Peptide Science 15(5), 403–423. https://doi.org/10.2174/1389203715666140512120605

St-Leger RJ, Screen SE, Shams-Pirzade B. 2000. Lack of Host Specialization in Aspergillus flavus. Applied and Environmental Microbiology 66(1), 320–324.  https://doi.org/10.1128/aem.66.1.320-324.2000

Thathana MG, Murage H, Abia AL, Pillay M. 2017. Morphological characterization and determination of aflatoxin-production potentials of aspergillus flavus isolated from maize and soil in kenya. Agriculture 7(80), 1-14. https://doi.org/10.3390/agriculture7100080

Wakil W, Ghazanfar MU, Yasin M. 2014. Naturally Occurring Entomopathogenic fungi infecting stored grain insect species in punjab, pakistan. Journal of Insect Sciences 14(82), 2-7.  https://doi.org/10.1093/jisesa/ieu044 

Zimmermann G. 1986. The Galleria bait method for detection of entomopathogenic fungi in soil. Journal of Applied Entomology 102(1-5), 213-215. https://doi.org/10.1111/j.1439-0418.1986.tb00912.x