Phenolic compounds and their antioxidant activities in Portulaca oleracea L. related to solvent extraction

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Research Paper 01/07/2017
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Phenolic compounds and their antioxidant activities in Portulaca oleracea L. related to solvent extraction

Samira Karoune, Mohamed Seif Allah Kechebar, Hadjer Douffi, Amir Djellouli
Int. J. Biosci.11( 1), 147-155, July 2017.
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Our work aims to explore and compare the biological evaluations of Portulaca oleracea leaves by the study of contents in phenolic compounds and antioxidant capacities in order to support scientifically its traditional interest using different solvent for optimization of the extraction. The first part of this study concerns the successive Soxhlet extraction and quantification of total phenols, flavonoid and condensed tannin by the reagent Folin-Ciocaleu, the aluminum trichloride and the vanillin test, respectively. The second part is the study of the antioxidant activities of plant extracts by using two in vitro methods: evolution of total antioxidant capacity and quenching of radical DPPH. The results showed that the methanol fraction is very rich in total polyphenols (13.41 EAG mg / g DM) and exhibits a good antioxidant activity especially for scanning free radicals (IC50 = 78.44 mg/ml). These results are encouraging for the recovery of this species as an excellent medicinal and alimentary plant.


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