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Research Paper | June 1, 2019

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Phenolic profile and antioxidant activity of Tribulus terristris polyphenols

Hina Anwar, Abdullah Ijaz Hussain, Shahzad Ali Shahid Chatha

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Int. J. Biosci.14(6), 405-414, June 2019

DOI: http://dx.doi.org/10.12692/ijb/14.6.405-414


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The aim of this study was identification and quantification of flavonoids and phenolic acids from various T. terristris (TT) extracts and evaluation of antioxidant and free radical scavenging activities. Different solvent systems were used to prepare TT extracts of different polarities. Maximum extract yield (14.32 g/100g 0f dry material) was recorded with 80% methanol solvent. Reverse phase high performance liquid chromatography (RP-HPLC) analysis of TT extracts revealed the presence of syringic acid, salicylic acid and gallic acid as major phenolic acids and catechin, myricetin, quercetin and Kaempferol as major flavonoids. Total phenolic (TP) and total flavonoid (TF) contents of TT extracts were measure as gallic acid equivalent (GAE) and catechine equivalent (CE), respectively. The significant variations (P< 0.05) in the amounts of TP and TF contents were observed in TT extract of different solvents. Maximum TP (38.01 mg/g of dry material, measured as GAE) and TF (11.91 mg/g of dry material, measured as CE) contents were recorded in 80% methanol extract of TT. Hexane extract showed the least TP and TF contents. The FA extracts showed excellent DPPH radical scavenging activity (IC50= 11.35 µg/ml), Inhibitation of linoleic acid peroxidation (89.40%), reducing power. The results are comparable with the synthetic antioxidants. The results showed the potential of TT as source of natural antioxidants.


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