Phenotypic study of lactic acid bacteria isolated from camel meat and milk

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Research Paper 01/04/2022
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Phenotypic study of lactic acid bacteria isolated from camel meat and milk

Benaissa Atika, Babelhadj Baaissa, Halimi Romaissa
Int. J. Biosci.20( 4), 121-131, April 2022.
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Abstract

The dromedary, a symbol of man’s survival in the desert, produces high-quality milk and meat. Because of their technological properties, lactic acid bacteria are used in a variety of food transformation processes. This study focused on the phenotypic identification of lactic acid bacteria isolated from camel meat, and milk in order to gain a better understanding of these lactic bacteria and to compare the bacterial genera found in the two other food products. Camel meat and milk samples were collected under sterile conditions. On MRS and M17 agar, the lactic flora is counted. The enumeration results revealed a bacterial richness of the milk samples compared to the meat samples, as well as a strong lactic load on M17 agar and MRS for both types of products examined. On M17 agar, the contamination rates of camel meat and milk are 4.43±0.80 logufc/g and 4.93±10.77 logufc/ml, respectively, and 4.40±0.96 logufc/g and 3.89±7.07 logufc/ml, respectively on MRS. The 11 lactic isolates of camel meat were obtained after phenotypic identification and were linked to five genera listed in order of dominance: Lactobacillus (36.36 %),  Lactococcus (27.27%), Streptococcus (18.18%), Enterococcus, and Pediococcus (9.09 %). While the 11 milk isolates were linked to five genera, they were listed in descending order: Lactococcus (54.55%), Lactobacillus (18.18%), Leuconostoc, Enterococcus, and Streptococcus (9.09 %). The Pediococcus genus has only been isolated from meat, whereas Leuconostoc has only been isolated from milk.

VIEWS 62

Ashmaig A, Hasan A, El Gaali E. 2009. Identification of lactic acid bacteria isolated from traditional Sudanese fermented camel’s milk (Gariss). African Journal of Microbiology Research 3(8), p 451-457. https://academicjournals.org/article/article1380279644_Ashmaig%20et%20al.pdf

Axelsson L. 2004.  Lactic Acid Bacteria: Classification and Physiology. In: Salminen, S, Wright AV, Ouwehand, A, Eds., Lactic Acid Bacteria: Microbiological and Functional Aspects, 3rd Edition, Marcel Dekker, New York, p 1-67. https://doi.org/10.1201/9780824752033.ch1 .

Bekhouche F. 2006. Lactic acid bacteria from raw cow’s milk and pectinolytic microorganisms from black and green olives: 1. Biochemical isolation and identification 2. Evaluation and optimization of the production of polygalacturonase enzymes. Thesis presented to obtain the degree of Doctor in Microbiology and Enzymology, Option: food engineering. University of Mentouri Constantine. Algeria.

Bouguerra A. 2021. Evaluation of the probiotic potential of lactic strains isolated from camel milk. Doctoral thesis in science. Major: Biology. Speciality: Microbiology Ferhat Abbas University, Serif . Algeria. http://dspace.univ.setif.dz:8888/jspui/bitstream/123456789/3785/1/bouguerra%20th%C3%A8se%20finale.pdf

Bouzaid M, Chatoui R, Latrache H, Hasib A. 2016. Antimicrobial activity of strains of lactic acid bacteria isolated from ground camel meat and raw cow’s milk. Revue. Microbiol. Ind. San et Environn. 10(1), p 1-12. https://www.researchgate.net/profile/Aziz-Hasib/publication/308174453

De Roissard HB. 1986. Bactéries lactiques. In lait et produits laitiers : vache, brebis, chèvre. Tome 3. Ed. Tec et Doc. Lavoisier. Apria, p 191-203.

De Vos P, Garrity G, Jones D, Krieg NR, Ludwig W, Rainey FA, Schleifer KH, Whitman W. 2009.  Bergey’s manual of Systematic Bacteriology. Volume 3, The Firmicutes. https://www.springer.com/gp/book/9780387950419

Dicks LMT, Van  Vuuren HJJ. 1987.  A modification of the hot-tube method for the detection of carbon dioxide produced by heterofermentative Lactobacillus strains. Journal of Microbiological Methods 6(5), p 273-275. https://www.sciencedirect.com/science/article/abs/pii/0167701287900649

Djeghri-Hocine B, Boukhemis M, Amrane A. 2010.  Formulation and Evaluation of a Selective Medium for Lactic Acid Bacteria-Validation on Some Dairy Products. American Journal of Agricultural and Biological Sciences 5(2), p 148-153. https://thescipub.com/abstract/10.3844/ajabssp.2010.148.153

Drici H, Gilbert C, Kihal M, Atlan D. 2010. Atypical citrate-fermenting Lactococcus lactis strains isolated from dromedary’s milk. Journal Applied Microbiology 108(2), p 647-657. https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/j.13652672.2009.04459.x

Faye B, Abdelhadi O, Raiymbek G, Kadim I, Hocquette J. 2013. The production of camel meat: State of knowledge, current situation and prospects. INRA productions animals 26(3), p 289-300. https://squ.pure.elsevier.com/en/publications/the-production-of-camel-meat-state-of-knowledge-current-situation

Ghozlane Dj. 2020. Isolement et caractérisation des bactéries lactiques productrices  d’arômes (diacétyle), magister : Science Alimentaire, Ecole national supérieured’agronomie El-Harrach, Algérie. http://dspace.ensa.dz:8080/jspui/bitstream/123456789/242/1/ghozlane_d.pdf

Guiraud JP. 1998. Techniques d’analyses microbiologiques : Microbiologie alimentaire. Technique et ingénierie. Série Agro-alimentaire  Dunod, Paris, France. https://www.worldcat.org/title/microbiologie-alimentaire/oclc/421905550

Guiraud JP. 2003. Microbiologie alimentaire. Technique et ingénierie, Paris : Dunod, série Agro-alimentaire. https://www.librairiedialogues.fr/livre/107946-microbiologie-alimentaire-joseph-pierre-guiraud-dunod

Hassaïne O, Zadi-Karam H, Karam NE. 2008. Phenotypic identification and technological properties of lactic acid bacteria isolated from three breeds dromedary raw milks in south Algeria. Emir. Journal  Food Agriculture  20(1), p 46-59. https://www.bibliomed.org/?mno=185549

ISO 6887-2. 2004. Microbiology of food and animal feeding stuffs – Preparation of test samples, initial suspension and decimal dilutions for microbiological examination – Part 2: Specific rules for the preparation of meat and meat products (ISO 6887-2:2003); German version EN ISO 6887-2:2003 (Foreign Standard). https://webstore.ansi.org/standards/din/dineniso68872004#PDF

Khedid K,  Faid M, Mokhtari A, Soulaymani A, Zinedine A. 2009. Characterization of lactic acid bacteria isolatedfrom the one humped camel milk produced in Morocco. Microbiological Research 164 (1), p 81-89 https://www.sciencedirect.com/science/article/pii/S0944501306001327

Klaenhammer TR, Rodolphe Barrangou R, Logan Buck B, Andrea M,  Peril  A, Altermann E. 2005.  Genomic features of lactic acid bacteria effecting bioprocessing and health. FEMS Microbiology Reviews 29, p 393–409. https://academic.oup.com/femsre/article/29/3/393/545187

Konuspayeva G, Faye B, Loiseau G. 2009. The composition of camel milk: A meta analyses of the literature data. Journal of Food Composition and Analysis 22, p 95-101. www.elsevier.com/locate/jfca

Lairini S, Beqqali N, Bouslamti R, Belkhou R, Zerrouq F. 2014. Isolement des bactéries lactiques à partir des produits laitiers traditionnels Marocains et formulation d’un lait fermenté proche du Kéfir. Afrique Science 10(4), p 267-277. http://www.afriquescience.info

Leonard L. 2013. Evaluation du potentiel bioprotecteur de bacteries Lactiques confinées dans une matrice polymérique. Thèse doctorat Sciences de l’Alimentation non publiée, Université de Bourgogne,France.

Liu SN, Han Y, Zhou ZJ. 2011. Lactic acid bacteria in traditional fermented Chinese foods. Food Research International 44(3), p 643-651. https://www.sciencedirect.com/science/article/abs/pii/S0963996911000056

Mozzi F, Raya RR, Vignolo GM. 2015. Biotechnology of Lactic Acid Bacteria: Novel Applications, 2nd Edition. Blackwell publishing, Singapore.

Mozzi F, Raya R, Vignolo G, Love JC. 2016. Biotechnology of Lactic Acid Bacteria–Novel Applications 2e. 4éme année. Université Montpellier II Sciences et Techniques du Languedoc 2, p 16-17.

Ouadghiri M,  Vancanneyt M,  Vandamme P,  Naser S,  Gevers D,  Lefebvre K,  Swings J,  Amar M. 2009.   Identification of lactic acid bacteria in Moroccan raw milk and traditionally fermented skimmed milk ‘lben’. Journal of Applied Microbiology 106(2), p 486-495. https://doi.org/10.1111/j.1365-2672.2008.04016.x

Raynaud Y. 2006. Régulation métabolique et trans  criptionelle de l’auto acidification chez Lactococcus lactis. Thèse de doctorat. L’institut national des sciences appliquées de Toulouse. https://core.ac.uk/download/pdf/35285149.pdf

Saidi D, Kihal M, Hammama A, Chakroun A, Henni DE, Kharoua O. 2005. Characterization of Algerian raw camel’s milk: identification of dominant lactic acid bacteria and proteins analysis. Journal Algerien des Regions Arides. l4(1), p 1-9. https://www.asjp.cerist.dz/en/downArticle/102/4/1/79061

Saidi Y. 2020. Biodiversité de la microflore lactique du lait cru de dromadaire et évaluation de ses caractères technologiques. Thèse de Doctorat. Spécialité : Microbiologie appliquée Contrôle microbiologique et hygiène alimentaire. Université Oran. Algérie. https://digital.csic.es/handle/10261/223956

Salminen S, Von Wright A, Ouwehand A. 2004. eds. Lactic acid bacteria: microbiological and functional aspects. 3rd. ed. New York: Marcel Dekker. https://www.scielo.br/j/rbcf/a/QBXvpJ6fkC5NjbtfrsTZzkL/?lang=pt

Stiles ME, Holzapfel WH. 1997. Lactic acid bacteria of foods and their current taxonomy. International journal of food microbiology 36(1), p  1-29. https://pubmed.ncbi.nlm.nih.gov/9168311

Trias R, Badosa E, Montesinosand E, Baneras L. 2008. Bioprotective Leuconostoc strains against Listeria monocytogenes in fresh fruits and vegetables. International Journal of food Microbiology 127(1-2), p 91-98. https://www.sciencedirect.com/science/article/abs/pii/S0168160508003218?via%3Dihub

Zadi Karam H, Karam NE. 2006.  Bactéries lactiques du lait de chamelle d’Algérie: mise en évidence de souches de Lactococcus résistantes au sel. Tropicultura 24(3), p 153-156. http://www.tropicultura.org/text/v24n3/153.pdf