Phytochemical screening and cytotoxicity of juices and bark extracts of sugarcane (Saccharum officinarum Linn) in Benin

Paper Details

Research Paper 01/05/2019
Views (293) Download (24)
current_issue_feature_image
publication_file

Phytochemical screening and cytotoxicity of juices and bark extracts of sugarcane (Saccharum officinarum Linn) in Benin

A. Adjatin, H.W. Djengue, O. Ekpelikpeze, Z. Bonougbo, J. Adomako, Assogba F, A. Dansi, J. Gbenou
Int. J. Biosci.14( 5), 95-105, May 2019.
Certificate: IJB 2019 [Generate Certificate]

Abstract

Sugarcane (Saccharum officinarum Linn) belongs to the Poaceae family and used widely for the preparation of traditional medicines in Benin. The present study was designed to evaluate phytochemical and cytotoxicity potential of seven sugarcane varieties. The precipitation and coloration method were used to determine phytochemical compounds whilst larval toxicity was performed using brine shrimp larvae (Artemia salina Leach). Results of the study revealed variation in phytochemical compounds in the different sugarcane varieties screened. Difference in phytochemical composition was observed also between the juice and bark of same sugarcane variety. Alkaloids, leuco-anthocyanins and reducing compounds were found in juice and bark from all sugarcane varieties used. The CL50 vary from 0.74 mg/mL to 2.66 mg/mL for sugarcane juice and from 0.28 mg/mL to 0.54 mg/mL for the bark extracts. Neither the juice nor bark of sugarcane has presented a risk of toxicity to consumers. The results of the study confirm the potency of sugar cane in preparation of indigenous food and medicine in Benin.

VIEWS 31

Abogo MAJ, Suebang FC, Ondo AAS, Yemelong JN, Ondo JA, Nsi EE, Eba F. 2013. Chemical composition of a standard sugarcane wine of Saccharum officinarum Linn from Woleu-Ntem, Gabon. Journal of Agriculture and Sustainability 3(2), 216-222.

Archimède H, Xande X, Gourdine JL, Fanchone A, Alexandre G, Boval M, Coppry O, Arquet R, Fleury J, Regnier C, Renaudeau D. 2011. La canne à sucre et ses co-produits dans l’alimentation animale. Innovations Agronomiques 16, 165-179.

Adjatin A, Dansi A, Eze CS, Assogba P, Dossou-Aminon I, Akpagana K, Akoègninou A, Sanni A. 2012. Ethnobotanical investigation and diversity of Gbolo [Crassocephalum rubens (Juss. ex Jacq.) S. Moore and C. crepidioides (Benth.) S. Moore], a traditional leafy vegetable under domestication in Benin. Genetic Resources and Crop Evolution 59(8), 1867-1881. http://dx.doi.org/10.1007/s10722-012-9901-z

Adjatin A, Dansi A, Badoussi E, Loko YL, Dansi M, Azokpota P, Gbaguidi F, Ahissou H, Akoègninou A, Akpagana K, Sanni A. 2013. Phytochemical screening and toxicity studies of Crassocephalum rubens(Juss. ex Jacq.) S. Moore and Crassocephalum crepidioides (Benth.) S. Moore consumed as vegetable in Benin. International Journalof Current Microbiology and Applied Sciences2 (8), 1-13.

Agbankpé AJ, Bankolé SH, Dougnon TJ, Yèhouénou B, Hounmanou YMG, Baba-Moussa LS. 2015. Comparison of Nutritional Values of Vernonia amygdalina, Crateva adansonii and Sesamum radiatum: Three Main Vegetables Used in Traditional Medicine for the Treatment of Bacterial Diarrhoea in Southern Benin (West Africa). Food and Public Health 54, 144-149. http://dx.doi.org/10.5923/j.fph.20150504.07.

Ahouansinkpo E, Atanasso J, Dansi A, Adjatin A, Orobiyi A, Sanni A. 2016. Ethnobotany, Phytochemical Screening and Toxicity Risk of Cleome gynandraand Cleome viscosa, two traditional leafy vegetables consumed in Benin.International Journal of Current Microbiology and Applied Sciences 5(2), 813-829. http://dx.doi.org/10.20546/ijcmas.2016.502.093

Allangba MRA, Kadja AB, Guessennd NK, Kouadio NJ,  Mamyrbékova-Békro JA, Békro YA. 2016. Etude phytochimique bio-guidée, activités antibactérienne et analgésique de décoctions éthanoliques d’une Mimosaceae de Côte d’Ivoire.  International Journal of Innovation and Applied Studies 18 (2), 569-577.

Banerji R, Madan VK, Misra SR. 1997. Preservation of sugarcane juice.Indian sugar 47(3), 195-200.

Boua BB, Mamyrbekova-Bekro JA, Kouame BA, Bekro YA. 2013. Criblage phytochimique et potentiel érectile de Turraea heterophylla de Côte d’Ivoire. Journal of Applied Biosciences 68, 5394 – 5403.

Carballo JL, Hernández-IndaZL, Pérez P, García-Grávalos MD. 2002. A comparison between two brine shrimp assays to detect in vitro cytotoxicity in marine natural products. BMC Biotechnology, 2, 17-22. https://doi.org/10.1186/1472-6750-2-17

Dansi A, Mignouna HD, Zoundjihékpon J, Sangare A, Ahoussou N, Asiedu R. 2000. Identification of some Benin Republic’s Guinea yam (Dioscorea cayenensis / Dioscorea rotundata complex) cultivars using Randomly Amplified Polymorphic DNA. Genetics Resources and Crop Evolution 47(6), 619-625.

Djengue HW, Dansi A, Assogba MF, Ahissou H, Adjatin A, Dansi  M, Gbénou DJ. 2017. Phytochemical Screening and Toxicity of Lippia multiflora Moldenke, a Minor Aromatic Leafy Vegetable Consumed in Benin. International Journal of Current Research in Biosciences and Plant Biology 4(5), 77-84. https://doi.org/10.20546/ijcrbp.2017.405.011

Doughari, JH. 2006. Antimicrobial Activity of Tamarindus indica Linn. Tropical Journal of Pharmaceutical Research 5(2), 597-603.

Edeoga HO, Omosun G, Uche LC. 2006. Chemical composition of Hyptissauveolens and Ocimumgratissium hybrids from Nigeria. African Journal of Biotechnology 5(10), 892-895.

Ekpélikpézé OS, Agre P, Dossou-Aminon I, Adjatin A, Dassou A. 2016a. Characterization of Sugarcane (Saccharum officinarum L.) Cultivars of Republic of Benin. International Journal of Current Research in Biosciences and Plant Biology 3(5), 147-156. http://dx.doi.org/10.20546/ijcrbp.2016.305.023

Ekpélikpézé OS, Loko YL, Adjatin A, Dansi A. 2016b. Evolution de la production de canne à sucre (Saccharum officinarum L.) et gestion paysanne de sa diversité variétale au Bénin. International Journal of Innovation and Scientific Research 20(2), 349-361.

Houghton PJ, Raman A. 1998. Laboratory Handbook for the Fractionation of Natural Extracts. Chapman and Hall, New York 5, 130-207.

Karthikeyan J, Samipillai S. 2010. Sugarcane in therapeutics. Journal of Herbal Medicine and Toxicology 4(1), 9–14.

Mahalingam R, Ambikapathy V, Panneer-Selvam A, Prince L. 2011. Biological activities of some medicinal plants against Setophaeria rostrata causing seeding blight disease in sugarcane. Asian Journal of Plant Science and Research 1(2), 92-95.

Makemba-Nkounkou GS, Nsonde-Ntandou GF, Peneme BML, Binimbi-Massengo A, Ouamba JM, Abena AA. 2017. Enquête ethnobotanique et effets de l’extrait aqueux des rhizomes de Anchomanes difformis Blume.Engl (Araceae) sur la motricité utérine de cobaye. Revue CAMES- Série Pharmacopée et Médecine Traditionnelle Africaine 18(2), 13-20.

Mira A, Syeda S, Farhana I, Dilara F,Rasheda A, Dilruba N. 2011. A survey of medicinal plants used the traditional medicinal practitioners of Khulna City, Bangladesh. American Eurasian Journal of Sustainable Agriculture 5, 177-195.

Molefe-Khamanga DM, Mooketsi NA, Matsabisa MG, Kensley RM. 2012. Qualitative phytochemical studies of solvent extracts from Myrothamnus flabellifolius. International Journal of Medicinal Plants Research 1, 15. http://onlineresearchjournals.org/OIJMPR/

Mousseux M. 1995. Test de toxicité sur les larves de Artemia salina et entretien d’un élevage de balanes, Rapport de stage de deuxième année. Deust Aquaculture; Centre Universitaire de Nouvelle-Calédonie, France.

Okwu DE. 2004.  Phytochemical  and  vitamin C content  of  indigenous species of South Eastern Nigeria. Journal of Sustainable Agriculture and Environmental 6, 30-34.

Okwu DE. 2007. Nigerian medicinal and aromatic plant. Science and Biotechnology 1(1), 90-96.

Orech FO, Kenga T, Ochora J, Friis H, Aagaard-Hansen J. 2005. Potential toxicity of some traditional leafy vegetables consumed in Nyang’oma Division, Western Kenya. African Journal of Food and Nutritional Sciences 5 (1), 1-14.

Pelka M, Danzyl C, Distler W, Petschelt A. 2000. A new screening test toxicity testing of dental materials.Journal of Dentistry 28(5), 341-345. https://doi.org/10.1016/S0300-5712(00)00007-5

Quignard EL, Pohlit AM, Nunomura SM, Pinto AC, Santos EV, Morais SK. 2003.Screening of plants found in Amazonas state for lethality towards brine shrimp. Acta Amazonica 33, 93-104.

Rajendran P, Bharathidasan R, Sureka I. 2017. Phytochemical Screening GC-MS and FT-IR Analysis of Sugarcane Juice. International Journal of Pharma Research and Health Sciences5 (6), 1962-67. http://dx.doi.org/10.21276/ijprhs.2017.06.12.

Sharma V, Paliwal R. 2013. Preliminary phytochemical investigation and TLC profiling of sequential extracts of Moringa oleifera pods. International Journal of green Pharmacy 7, 41-45.

Souza DRD, Stingel E, Almeida LCD, Munhae CB, Mayhe-Nunes AJ. 2010. Ant diversity in a sugarcane culture without the use of straw burning in southeast, Sao Paulo, Brazil. American Journal of Agricultural Biological Sciences 5(2), 183-188.

Uchenna EF, Okechukwu AA, Adindu CS. 2015. Phytochemical and Antimicrobial Properties of the Aqueous Ethanolic Extract of Saccharum officinarum (Sugarcane) Bark.Journal of Agricultural Science 7(10), 291-297. http://dx.doi.org/10.5539/jas.v7n10p291

Vincent K. 2012. Probit Analysis. http://userwww.sfsu.edu

Zohra SF, Meriem B, Samira S, Muneer AMS. 2012. Phytochemical Screening and identification of some compounds from Mallow. Journal Natural of Production and Plant Resources 2(4), 512-516.