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Polyphenolic content and antioxidant activity of hydroalcohlic and alcoholic extract of Thymus vulgaris

Anoosh Eghdami, Mojtaba Eizadi, Fatemeh Sadeghi

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J. Bio. Env. Sci.3(5), 94-101, May 2013

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Abstract

Thymus vulgaris (thyme) (like some other wild Thymus species (wild thyme)) possesses a wide range of biological activities including expectorant, spasmolitic, sedative, antibacterial, antifungal, antioxidant. The objective of this paper was a screening of flavonoid content in Thymus vulgaris, the methanol extract of plant material was used for High-performance liquid chromatography (HPLC) analysis. Additionally, the antioxidant activities were also determined by ferric reducing antioxidant power (FRAP), 1, 1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity and reducing power methods. Hydroalcoholic extract had the higher flavonoid and total phenolic contents than alcoholic extract of Thymus vulgaris .A strong positive correlation of R2 = 0.92 between total phenolic content and antioxidant activity was observed in this investigation. This study indicated that Thymus vulgaris exhibited the high antioxidant activity, flavonoid and phenolic content and can be used potentially as a readily accessible source of natural antioxidant.Antioxidant activities of polyphenols have been suggested to exert beneficial pharmacological effects on some diseases.

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Polyphenolic content and antioxidant activity of hydroalcohlic and alcoholic extract of Thymus vulgaris

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