Protease activity in Labeo rohita fingerlings fed Lactobacillus acidophilus and Spirulina platensis
Paper Details
Protease activity in Labeo rohita fingerlings fed Lactobacillus acidophilus and Spirulina platensis
Abstract
In the present 75-day experiment, protease activity was studied in the intestinal tissue of fingerlings of the freshwater fish Labeo rohita. The fish were fed diets containing 2%, 4%, and 6% concentrations at the rate of 3% of their body weight at 15-day intervals. The 210 fingerlings were divided into 4 groups; Fish group T0 (control diet) were fed a diet containing feed ingredients without experimental additives. Fish groups T1L, T2L, and T3L (experimental diets) were fed diets containing 2%, 4%, and 6% Lactobacillus acidophilus tablet (SPORALAC-DS) powder, in that order. Fish groups T1S, T2S, and T3S were fed diets containing 2%, 4%, and 6% Spirulina platensis powder, respectively. After the 75-day period, total protease was measured in the intestine of the fingerlings. It was observed that intestinal enzyme activity increased over time in both the control and experimental groups. Protease activity was highest in the 6% diet concentration groups, with significant increases seen in the Spirulina platensis-fed fingerlings compared to the control group and other groups. The results indicate that protease activity was highest in the group fed 6% Spirulina platensis (T3S), followed by the group fed 6% Lactobacillus acidophilus (T3L). This suggests that a 6% dietary concentration of either Spirulina platensis or Lactobacillus acidophilus can help enhance protease activity and growth in L. rohita fingerlings, with S. platensis showing greater efficacy.
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