Protease enzyme reactability effect of pearl oyster (Pinctada maxima) flesh shell growth at different water temperature and salinity

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Research Paper 01/07/2016
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Protease enzyme reactability effect of pearl oyster (Pinctada maxima) flesh shell growth at different water temperature and salinity

M. S. Hamzah, Yuli H. Endang, Marsoedi, M. Fadjar
Int. J. Biosci.9( 1), 124-137, July 2016.
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Abstract

Shell growth of pearl oyster is affected by the availability of nacreous liquid containing calcium carbonate (CaCO3). It is nearly similar to mucus from enzymatic metabolism product in protein digestion. This study was aimed at observing the protease enzyme reactability and its effect on the survival and growth rate of the pearl oyster seeds at different water temperature and salinity conditions. It was carried out on March 6th – May 7th, 2015, at Laboratory of Marine Bio-industry Technical Implementation Unit, Research Centre for Oseanography, Indonesian Institute of Sciences in Lombok. CaCO3 concentration was analyzed using an Atomic Absorption Spectrophotometer (AAS) to know the reactability of protease enzyme and the relationship between CaCO3 content and shell growth. ANOVA indicated that larval survival at spat phase cultured in the media of different temperature-salinity interaction showed different effect (P<0.05). Based on Honest Significant Difference test, spat survival at the treatment of tempersture-salinity interaction II (28±0.5ºC and 32±1ppt) was the best. High spat survival and growth in treatment II were supported by the reactability of protease enzyme and higher CaCO3, 0.0518 µmol/mL-min-1(unit) and 98.873 ±0.04% than those of other treatments. In addition, water quality of the culture media was also discussed in this paper.

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Blank S, Arnoldi M, Khoshnavaz S,Treccani L, Kuntz M, Mann K, Grathwohl G, Fritz M. 2003. The nacre protein perlucin nucleates growth calcium carbonate crystals. Journal of Microscopy 212, 280-291. http://dx.doi.org/10.1111/j.1365-2818.2003.01263.x

Bedouet L, Schuller MJ, Marin F, Milet C, Lopez E, Giraund M. 2001. Soluble proteins of the nacre of the giant oyster Pinctada maxima and of the abalone Haliotis tuberculata: extration and partial analysis of nacre protein. Comparative Biochemistry and Physiology Part B 128, 389-400. http://dx.doi.org/10.1016/S1096-4959(00)00337-7

Caiping, M, Cen Z, Yancheng N, Liping X, Rongqing Z. 2005. Extraction and purification of matrix protein from the nacre of pearl oyster Pinctada fucata. Tsinghua Science Technology 10(4), 499-503. http://dx.doi.org/10.1016/S1007-0214(05)70107-3

Feng Q, Fang Zi, Yan Z, Xing R, Xie L, Zhang R. 2009. The structure-fungsional relationship of MS17, a matrix protein from pearl oyster Pinctada fucata. Acta Biochim Biophys Sin 41(11), 955-962. http://dx.doi.org/10.1093/abbs/gmp086

Gong N, Zhuojun Ma, Qing Li, Qi Li, Yan Z, Xie L, Zhang R. 2008. Characterization of calcium deposition and shell matrix protein secretion in primary mantle tissue cuture from the marine pearl oyster Pinctada maxima. Marine Biotechnology 10, 457-465. http://dx.doi.org/10.1007/s10126-008-9081-1

Hamzah MS. 1997. Study on embryonic development and hatchability of squid eggs (Sepioteuthis lessoniana, LESSON) at different temperature and salinity conditions. Thesis. Study Program of Agriculture system, Fisheries Studies.Master Program of Hasanuddin University, Ujung Pandang, 79 p.

Hamzah MS. 2003. The effect of spat collector color on the attachibility of pearl oyster (Pinctada maxima) larvae in rearing tank. In : Buku Pesisir dan Pantai Indonesia VIII. Ruytno, Pramudji and Imam Supangat (eds). Research Centre for Oseanography, Indonesian Institute of Sciences in Jakarta, 79-84.

Hamzah MS. 2007. The effect of depth level on the attachibility of mabe oyster (Pteria penguin) larvae to the net as spat collector in Kapontori Bay, Buton Regency-Southeast Sulawesi. In : Proceeding of National Seminar on Mollusc in research, conservation, and economy. JournalMarine Science, Faculty Fisheries And Marine Sciences Semarang, 134-141.

Hamzah MS, Setiyono DE. 2009. Study on growth and survivorhip of pearl oyster seeds (Pinctada maxima) at different water temperature conditions. Proceeding on Annual Scientific Meeting (ISOI), 2008 in Bandung. Indonesian Oceanologist Association, 240-246.

Hamzah MS. 2013. Attachibility of pearl oyster (Pinctada maxima) larvae to the collectors under different stocking position and depth. Jurnal Tropical Marine Sciences And Technology 5(1), 60-68.

Hamzah MS. 2015. Survival and growth of gold-mouth turban(Turbo chrysostomus L.) at different temperature condition. Journal. Tropical marine Sciences and Technology 7 (1), 299-308.

Hamester MRR, Balze PS, Becker D. 2012. Characterization of calcium carbonate obtained from oyster and mussel shells and incorporation in Polypropylene. Materials Research 15 (2), 204-208. http://dx.doi.org/10.1590/S151614392012005000014

Haws M, Ellis S. 2000. Aquafarmer information sheet : collectiong black-lipped pearl oyster spat. Resources Center Univ. Of Hawaii at Hilo, HI 96720 USA. Center for Tropical and Subtropical Aquaculture CTSA Publication No. 144.

Islami MM. 2012. Several bio-ecological aspects of molluscs in relation with tide conditions. Fauna Indonesia. Research Centre for Biology, Indonesian Institute of Sciences in Bogor 11(1), 37-43.

KMNLH. 2004. Decree of State Minister of Population and Environment No. 51, 2004 concerning seawater quality standard. Office of Living Environment, Jakarta 4p.

Kvingedal R, Evans BS, Taylor JJU, Knauer J, Jerry DR. 2008. Family by environment interactions in shell size of 43-day old silver-lip pearl oyster (Pinctada maxima), five families reared under different nursery conditions. Aquaculture 279, 23-28. http://dx.doi.org/10.1016/j.aquaculture.2008.04.022

Litaay M. 2011. Lola Trochus nilotiucus L. culture. Reflection of Indonesia shell culture development. Research and Development Board for Marine and Fisheries. Research and Development Center of Culture Fisheries, Jakarta, 149-163.

Miyamoto H, Miyoshi F, Kohno J. 2005. The carbonic anhydrase domain protein nacrein is expressed in the epithelial cell of the mantle and acts as a negative regulator in calcification in the Molluscs Pinctada fucata. Zoological Science of Japan 22, 311-315. http://dx.doi.org/10.2108/zsj.22.311

Mouries LP, Almeida MJ, Ribeiro C, Peduzzi J, Barthelemy M, Milet C, Lopez E. 2002. Soluble silk-like organic matrix in the nacreous layer the bivalve Pinctada maxima. Eur. Journal Biochimestry 269, 4994-5003. http://dx.doi.org/10.1046/j.1432-1033.2002.03203.x

Naganuma T, Hoshino W, Shikanai Y, Sato R, Liu K, Muramoto K, Osada M, Yoshimi K, Ogawa T, Sato S. 2014. Novel Matrix Proteins of Pteria penguin Pearl Oyster Shell Nacre Homologous to  the  Jacalin-Related  ß-Prism Prism Fold  Lectin. PLoS ONE 9(11), e112326. http://dx.doi.org/10.1371/journal.pone.0112326

O’Connor WA, Lawler NF. 2004. Salinity and temperature tolerance of embryos and juveniles of the pearl oyster, Pinctada imbricata Ro¨ding. NSW Fisheries, Port Stephens Fisheries Centre, Taylors Beach, NSW 2316, Australia. Aquaculture 229, 493–506. http://dx.doi.org/10.1016/S0044-8486(03)00400-9

Simonian MH. 2002. Spectrophotometric determination of protein concentration, John Willey & Sons. Inc. http://dx.doi.org/10.1002/0471140864.ps0301s33

Sudjana. 1991. Design and experimental analyses, 3rd ed. Published by “Tarsito” Bandung, 415 p.

Supii AI. 2007. Breeding experiment of pearl oyster (Pinctada maxima) in backyard hatchery scale. Proceeding of Marine National Seminar III, Univ. Hang Tuah.Science-Tecnology-based marine developmentin order to increase coastal community welfare. Surabaya, 49-58.

Zheng H, Zhang C, Cao W, Liu S, Ji H. 2009. Preparation and chracterisation of the pearl oyster (Pinctada martensii) meat protein hydrolysates with a high Fischer ratio. International Jounal of. of Food Science and Technology 44, 1183-1191. http://dx.doi.org/10.1111/j.1365-2621.2009.01942.x