Protective effect of Asparagus adscendens on 5-fluorouracil induced gastrointestinal lesions in Wistar rats
Paper Details
Protective effect of Asparagus adscendens on 5-fluorouracil induced gastrointestinal lesions in Wistar rats
Abstract
The use of the anti-cancer drug 5-fluorouracil (5-FU) is known to induce intestinal mucositis in patients without known satisfactory preventive therapy. An ethanolic extract of Asparagus adscendens (A. a. EE) of was prepared with extractability percentage calculated as 27.2% (w/w) anda simple solution was made using CMC (carboxy methyl cellulose).Twenty-four five-week-old male Wistar rats were divided in four groups- Group-I acted as control(receiving oral saline), Group-II receiving 5-FU, Group-III receiving 5-FU and Asparagus adscendens EE, and lastly Group-IV receiving Asparagus adscendens EE alone. Dosage of Asparagus adscendens EE (300mg/kg body weight) was prepared in three divided doses and administered by gavage to the rats. A single intraperitoneal dose of 5-FU (500 g/ml) was injected in the test groups II and III. A drastic and significant (p<0.01) fall in body weight between Group-II and III rats, together with severe diarrhoea score in these groups was noted. The histopathological scores derived from 11 devised parameters showed a severe and significant (p<0.01) affliction of the ileum as compared to the jejunum in Groups-II and III. With 5-FU administration, histopathological changes in the jejunum were associated with severe pathology, such as- villi shortening, disruption of tips, degenerate enterocytes, Goblet cell reduction, marked mucosal mononuclear cell infiltration, significant loss of crypts or their distortion/loss, congestion of blood vessels and submucosal oedema. Similar changes also appeared in the ileum, together with lacteal dilation and hyperplastic Peyer’s patches. In Group-III, enteric changes in the jejunum and ileum were indicative of mild enteritis, although far less in severity than observed in Group-II. In Group-IV changes were normal except for abundant Goblet cells, both in the jejunum and ileum. Overall Asparagus adscendens was found to significantly improve the detrimental effects induced by 5-FU.
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