Response of different soybean varieties to phosphorus fertilizer microdosing and rhizobium inoculation in the sub-humid zone of Northern Benin

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Research Paper 23/12/2025
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Response of different soybean varieties to phosphorus fertilizer microdosing and rhizobium inoculation in the sub-humid zone of Northern Benin

Pierre G. Tovihoudji*, Kamarou-Dine Seydou, Lionel Zadji, Sissou Zakari, Valerien A. Zinsou
Int. J. Biosci. 27(6), 201-213, December 2025.
Copyright Statement: Copyright 2025; The Author(s).
License: CC BY-NC 4.0

Abstract

Soybean cultivation has expanded among farmers in Benin, yet yields remain below potential, mainly due to declining soil fertility and poor seed quality. This study evaluated the effects of organo-mineral microdose fertilization and rhizobium inoculation on the agronomic performance of three soybean varieties in the sub-humid zone of Northern Benin over two consecutive growing seasons. A split-plot design with three replications was used. The main factor was soybean variety, consisting of one traditional variety and two improved varieties (TGX1910-14F and TGX1830-20E). The sub-factor was fertilization, with nine treatments: i) control (no fertilizer, manure or inoculum), ii) phosphorus microdosing (MD; 10 kg P/ha), iii) recommended phosphorus rate (RR; 33 kg P/ha), iv) rhizobium inoculation (I), v) MD+I, vi) farmyard manure (F; 3 t/ha), vii) MD+F, viii) F+I, and ix) MD+I+F. The results showed that variety, fertilization and their interaction significantly affected soybean yield parameters. The improved variety TGX1910-14F produced the highest grain yield (2492 kg/ha), outperforming both the traditional variety and TGX1830-20E. The RR, MD+I+F and MD+F treatments resulted in the greatest yield improvements, with increases of approximately 95%, 100% and 103% compared to the control, respectively. Economic analysis indicated that these same treatments also generated the highest gross margins, with increases of 140%, 142% and 149% compared to the unfertilized control. Overall, the combined application of microdose phosphorus with inoculum, manure or both, particularly when applied to the TGX1910-14F variety, appears to be an effective strategy for improving soybean productivity and farmer income in Northern Benin.

Abbasi MK, Manzoor M, Tahir MM. 2010. Efficiency of Rhizobium inoculation and P   fertilization in enhancing nodulation, seed yield, and phosphorus use efficiency by field grown soybean under hilly region of Rawalakot Azad Jammu and Kashmir, Pakistan. Journal of Plant Nutrition. Taylor & Francis. https://doi.org/10.1080/01904161003729782

Adimi OB, Dassoundo-Assogba CFJ, Kinkpe AT, Yabi AJ. 2017. Éducation en production de soja au nord-est du Bénin. Bulletin de la Recherche Agronomique du Bénin, Numéro spécial Technologie Alimentaire & Sécurité Alimentaire. Institut National des Recherches Agricoles du Bénin.

Aduloju MO, Mahamood J, Abayomi YA. 2009. Evaluation of soybean [Glycine max (L) Merrill] genotypes for adaptability to a southern Guinea savanna environment with and without P fertilizer application in north central Nigeria. African Journal of Agricultural Research. Academic Journals. https://eprints.lmu.edu.ng/id/eprint/2675

Afzal A, Asghari B, Mussarat F. 2010. Higher soybean yield by inoculation with N- fixing and P-solubilizing bacteria. Agronomy for Sustainable Development. EDP Sciences S.A. https://doi.org/10.1051/agro/2009041

Agnoro M. 2008. Effet de l’inoculation avec” Bradyrhizobium japonicum” et de l’apport de phosphore sur la productivité du soja (Glycine max) en champs paysans au Bénin. Oléoscope. Université d’Abomey Calavi, Faculté des Sciences Agronomiques.

Ahiabor BDK, Lamptey S, Yeboah S, Bahari V. 2014. Application of phosphorus fertilizer on soybean (Glycine max L. (Merril) inoculated with rhizobium and its economic implication to farmers. American Journal of Experimental Agriculture. ScienceDomain International.  http://hdl.handle.net/123456789/3221

Amidou M, Djènontin AJ, Wennink B. 2005. Valorisation des résidus de récolte dans l’exploitation agricole au nord du Bénin : utilisation du fumier produit dans le parc de stabulation des bœufs. Bulletin de la Recherche Agronomique du Bénin. Institut National des Recherches Agricoles du Bénin.

Bambani RC, Vodouhe F, Alaye E. 2024. Efficacité économique des producteurs de maïs et du soja adoptant la gestion intégrée de fertilité des sols comme technique d’adaptation aux changements climatiques au Nord du Bénin. Revue Marocaine des Sciences Agronomiques et Vétérinaires. AgriMaroc. https://doi.org/10.5281/zenodo.10798961

Belete S, Bezabih M, Abdulkadir B, Tolera A, Mekonnen K, Wolde-Meskel E. 2019. Inoculation and phosphorus fertilizer improve food-feed traits of grain legumes in mixed crop-livestock systems of Ethiopia. Agriculture, ecosystems & environment. Elsevier. https://doi.org/10.1016/j.agee.2019.04.014

Buerkert A, Bationo A, Piepho HP. 2001. Efficient phosphorus application strategies for increased crop production in sub-Saharan West Africa. Field Crops Research. Elsevier. https://doi.org/10.1016/S0378-4290(01)00166-6

Buruchara R, Chirwa R, Sperling L, Mukankusi C, Rubyogo J, Mutonhi R, Abang M. 2012. Development and delivery of bean varieties in Africa: The Pan-Africa bean research alliance (PABRA) model. African Crop Science Journal. African Crop Science Society. https://doi.org/10.4314/acsj.v19i4.

Couto C, Silva LR, Valentão P, Velázquez E, Peix A, Andrade PB. 2011.  Effects induced by the nodulation with Bradyrhizobium japonicum on Glycine max (soybean) metabolism and antioxidant potential. Food Chemistry. Elsevier. https://doi.org/10.1016/j.foodchem.2011.01.135

Ferguson BJ, Mens C, Hastwell AH, Zhang M, Su H, Jones CH, Gresshoff PM. 2019. Legume nodulation: The host controls the party. Plant, cell & environment. Wiley. https://doi.org/10.1111/pce.13348

Gee GW, Or D. 2002. 2.4 Particle‐size analysis. Methods of soil analysis: Part 4 physical methods. Wiley. https://doi.org/10.2136/sssabookser5.4.c12

Hayashi K, Abdoulaye T, Gerard B, Bationo A. 2008. Evaluation of application timing in fertilizer micro-dosing technology on millet production in Niger, West Africa. Nutrient Cycling in Agroecosystems. Springer Nature. https://doi.org/10.1007/s10705-007-9141-3

Houngnandan P, Zapata F, Boekx P, Van Cleemput O. 2010. Effect of bradyrhizobial inoculation and n fertilization on promiscuous soybean and subsequent maize yield grown in degraded “terre de barre” in Benin. Annales Des Sciences Agronomiques. Institut National des Recherches Agricoles du Bénin. https://doi.org/10.4314/asab.v12i2.53855

Hussain K, Islam M, Siddique MT, Hayat R, Mohsan S. 2011. Soybean Growth and Nitrogen Fixation as Affected by Sulfur Fertilization and Inoculation under Rainfed Conditions in Pakistan. International Journal of Agriculture & Biology. Friends Science Publishers.

Ibrahim A, Abaidoo RC, Fatondji D, Opoku A. 2015. Hill placement of manure and fertilizer micro-dosing improves yield and water use efficiency in the Sahelian low input millet-based cropping system. Field Crops Research. Elsevier. https://doi.org/10.1016/j.fcr.2015.04.022

Ikeogu UN, Nwofia GE. 2013. Yield parameters and stabilty of soybean [Glycine max (L.) merril] as influenced by phosphorus fertilizer rates in two ultisols. Journal of Plant Breeding and Crop Science. Academic Journals. https://doi.org/10.5897/JPBCS12.014

Kabiru MR, Buernor AB, Dahhani S, Hafidi M, Jibrin JM, Jemo M. 2024. Soybean yield variability and predictability from applied phosphorus sources and rhizobia inoculation in Northern Nigeria. Frontiers in Sustainable Food Systems. Frontiers Media SA. https://doi.org/10.3389/fsufs.2024.1428466

Liu Q, Xu H, Mu X, Zhao G, Gao P, Sun W. 2020. Effects of different fertilization regimes on crop yield and soil water use efficiency of millet and soybean. Sustainability. MDPI. https://doi.org/10.3390/su12104125

Liu Y, Bai M, Shen F, Wu Z, Yang J, Li N, Han X. 2024. Enhancing Soybean and Maize Yields through Improved Nitrogen and Soil Water Use Efficiencies: A 40-Year Study on the Impact of Farmyard Manure Amendment in Northeast China. Plants. MDPI. https://doi.org/10.3390/plants13040500

Lyimo LD, Tamba MR, Madege RR. 2017. Effects of genotype on yield and yield component of soybean (Glycine max (L) Merrill). African Journal of Agricultural Research. Academic Journals. https://doi.org/10.5897/AJAR2017.12177

MAEP. 2014. Cellule de suivi- Rapport de performance du secteur agricole. MAEP Bénin.

McInnes A, Haq K. 2007. Contributions of Rhizobia to Soil Nitrogen Fertility. In Soil Biological Fertility -A Key to Sustainable Land Use in Agriculture (Eds) Abbott, L.K. and D.V. Murphy. Springer Nature.

Mühlig-Versen B, Bürkert A, Bationo A, Roemheld V. 2003. Phosphorus placement on acid arenosols of the West African Sahel. Experimental Agriculture. Cambridge University Press. https://doi.org/10.1017/S0014479703001261

Okogun JA, Sanginga N, Abaidoo R, Dashiell KE, Diels J. 2005. On-farm evaluation of biological nitrogen fixation potential and grain yield of Lablab and two soybean varieties in the northern Guinea savanna of Nigeria. Nutrient cycling in agroecosystems. Springer Nature. https://doi.org/10.1007/s10705-005-3821-7

Ronner E, Franke AC, Vanlauwe B, Dianda M, Edeh E, Ukem B, Giller KV. 2016. Understanding variability in soybean yield and response to P-fertilizer and rhizobium inoculants on farmers’ fields in northern Nigeria. Field Crops Research. Elsevier. https://doi.org/10.1016/j.fcr.2015.10.023

Smaling EMA, Roscoe R, Lesschen JP, Bouwman AF, Comunello E. 2008. From forest to waste: Assessment of the Brazilian soybean chain, using nitrogen as a marker. Agriculture, Ecosystems & Environment. Elsevier. https://doi.org/10.1016/j.agee.2008.06.005

Tairo EV, Ndakidemi PA. 2014. Macronutrients uptake in soybean as affected by Bradyrhizobium japonicum inoculation and phosphorus (P) supplements. American Journal of Plant Sciences. Scientific Research Publishing.

Tovihoudji PG, Akponikpè PI, Adjogboto A, Djenontin JA, Agbossou EK, Bielders CL. 2018. Combining hill-placed manure and mineral fertilizer enhances maize productivity and profitability in northern Benin. Nutrient Cycling in Agroecosystems. Springer Nature. https://doi.org/10.1007/s10705-017-9872-8

Tovihoudji PG, Akponikpè PI, Agbossou EK, Bertin P, Bielders CL. 2017. Fertilizer microdosing enhances maize yields but may exacerbate nutrient mining in maize cropping systems in northern Benin. Field crops research. Elsevier. https://doi.org/10.1016/j.fcr.2017.08.003

Twomlow S, Rohrbach D, Dimes J, Rusike J, Mupangwa W, Ncube B, Mahposa P. 2011. Micro-dosing as a pathway to Africa’s Green Revolution: evidence from broad-scale on-farm trials. In Innovations as Key to the Green Revolution in Africa: Exploring the Scientific Facts. Dordrecht: Springer Netherlands. https://doi.org/10.1007/978-90-481-2543-2_113

Ulzen J, Abaidoo RC, Mensah NE, Masso C, AbdelGadir AH. 2016. Bradyrhizobium inoculants enhance grain yields of soybean and cowpea in Northern Ghana. Frontiers in plant science. Frontiers Media SA. https://doi.org/10.3389/fpls.2016.01770

Ulzen J, Abaidoo RC, Ewusi-Mensah N, Masso C. 2018. On-farm evaluation and determination of sources of variability of soybean response to Bradyrhizobium inoculation and phosphorus fertilizer in northern Ghana. Agriculture, Ecosystems & Environment. Elsevier. https://doi.org/10.1016/j.agee.2018.08.007

Ulzen J, Abaidoo RC, Ewusi-Mensah N, Osei O, Masso C, Opoku A. 2020. Organic manure improves soybean response to rhizobia inoculant and P-fertilizer in northern Ghana. Frontiers in Agronomy. Frontiers Media SA. https://doi.org/10.3389/fagro.2020.00009

van Heerwaarden J, Baijukya F, Kyei-Boahen S, Adjei-Nsiah S, Ebanyat P, Kamai N, Giller K. 2018. Soyabean response to rhizobium inoculation across sub-Saharan Africa: Patterns of variation and the role of promiscuity. Agriculture, ecosystems & environment. Elsevier. https://doi.org/10.1016/j.agee.2017.08.016

Van Reeuwijk LP. 2002. Procedures for soil analysis (6th edition). Technical Paper, ISRIC, Wageningen.

Walkley A, Black IA. 1934. An examination of the Degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil science. Williams & Wilkins.

Wang C, Sun A, jie Zhu L, Liu M, Zhang Q, Wang L, Gao X. 2025. Drought and rewatering effects on soybean photosynthesis, physiology and yield. Plant Biology. PeerJ. https://doi.org/10.7717/peerj.19658

Wei W, Guan D, Ma M, Jiang X, Fan F, Meng F, Li J. 2023. Long-term fertilization coupled with rhizobium inoculation promotes soybean yield and alters soil bacterial community composition. Frontiers in Microbiology. Frontiers Media SA. https://doi.org/10.3389/fmicb.2023.1161983

Youssouf I, Lawani M. 2002. Les sols béninois : classification dans la base de référence mondiale. Quatorzième réunion du sous-comité ouest et centre africain de corrélation des sols pour la mise en valeur des terres, 9–13 octobre 2000 Abomey, Bénin. Rapport sur les Ressources en Sols du Monde. FAO, Rome.

Zoundji CC, Houngnandan P, Amidou MH, Kouelo FA, Toukourou F. 2015. Inoculation and phosphorus application effects on soybean [Glycine max (L.) merrill] productivity grown in farmers ‘fields of Benin. Journal of Animal & Plant Sciences. Academia.

Zwart SJ, Bastiaanssen WG. 2004. Review of measured crop water productivity values for irrigated wheat, rice, cotton and maize. Agricultural water management. Elsevier. https://doi.org/10.1016/j.agwat.2004.04.007

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