Screening of bacteriocin producing bacteria obtained from marine soil sediments and their antibacterial activity against diabetic foot ulcer pathogens
Paper Details
Screening of bacteriocin producing bacteria obtained from marine soil sediments and their antibacterial activity against diabetic foot ulcer pathogens
Abstract
Marine bacteria are an important and relatively unexplored resource for novel microbial products. Bacteriocins were first detected by Andre Gratia in 1925. Bacteriocins function as a natural bacterial immune weapon system. Gram-positive and Gram-negative can produce many kinds of bacteriocins that allow bacteriocin-producing bacteria to have the ability to inhibit the growth of sensitive bacteria. They are ribosomally synthesized extracellularly released low molecular mass peptides, produced by different types of bacteria, Gram-positive, Gram-negative and Archaea. They can be produced spontaneously or induced by certain chemicals such as mitomycin C. In this study, several bacterial strains were isolated from the soil and screened for bacteriocin production. This newly isolated strain showed antibacterial activity against several Gram-positive and Gram-negative bacteria. Different concentrations of tryptone, yeast extract, and NaCl and physiochemical factors such as temperature, pH, and incubation period were selected as variables for maximum production of bacteriocin by using the agar well diffusion method. Totally19 bacterial colonies were isolated from three different marine environments of different places mangrove, saltpan, and coastal area of kodiyakarai area in vedaranyam, Nagapattinam district of Tamil Nadu. Probiotic properties viz., resistance to low pH and tolerance against bile salt 0.3% were tested. Six isolates had shown antibacterial activity against identified pathogens such as Enterococcus sp, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus pneumoniae.
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A. Swedha, G. Kanimozhi, A. Panneerselvam, 2024. Screening of bacteriocin producing bacteria obtained from marine soil sediments and their antibacterial activity against diabetic foot ulcer pathogens. Int. J. Biosci., 24(2), 97-102.
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