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Seed germination potential, phytochemical analysis and antioxidant activity of two tomato varieties

Mst. Samima Nasrin, Fatema Jesmin, M. Mostafizur Rahman, M. Firoz Alam

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Int. J. Biosci.8(1), 63-76, January 2016

DOI: http://dx.doi.org/10.12692/ijb/8.1.63-76


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The present study has carried out to evaluate seed germination potential, phytochemical analysis and antioxidant activity of two tomato varieties viz., Raton and Persimmon.Seed germination experiment has undertaken with an objective to determine how the rate of seed germination can be influenced by various concentrations (10-3M, 10-4M, 10-5M) of plant hormones i.e. NAAand BA.Raton (BA 10-5M) has showed highest germination percentage as well as the longestshoot and root length in contrast to other treatments. So, low concentration of plant hormones has showed more effectiveness for seed germination in both varieties. Phytochemical analysis and antioxidant activityof well ripen berry powder have assessed to explainthe scientific basis fornutritional and nutraceuticals benefits of tomato. Phytochemical screening of Raton and Persimmon have showed that alkaloids, flavonoids, tannins, terpenoids and glycosides are present in both Raton and Persimmon, but saponin and phenol are absent in Persimmon. Nutraceutical especially antioxidant activities of Raton and Persimmon have examined through DPPH free radical scavenging assay. Both the varieties have showed significant antioxidant activity. Where IC50 value of Raton and Persimmon are 29.2 mg/ml and 38.1 mg/ml respectively. The IC50 value of standard sample (ascorbic acid) is 11.5 mg/ml. The result of this study indicates that Raton contain higher antioxidant potential than Persimmon.The phytochemical analysis supportsantioxidant properties of both varieties and also indicating for developing herbal medicine from tomatoes.


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Seed germination potential, phytochemical analysis and antioxidant activity of two tomato varieties

Adnan JM, Zearah SA, Alwan NA. 2013. Total antioxidant capacity and antihyperlipidemic activity of alkaloid extract from aerial part of Anethumgraveolens L. plant. European Scientific Journal 9(33), 1857-7881.

Ahmad I, Beg Z. 2001. Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. Journal ofEthnopharmacology 74, 87-91.

Akilan CA, Srividhya M, Mohana PC, Jeba SCS,Sundara MMA. 2014. Comparative Analysis ofPhytochemicals, Antibiogram of Selected Plants in Solanaceae Family and its Characterization Studies. International Journal of Pharmacy and PharmaceuticalSciences 6(2), 946-950.

Akueshi CO, Kadiri CO, Akueshi EU, Agina SE, Ngurukwem B. 2002. Antimicrobial potentials of HyptissauvedensPoit(Lamiaceae).Nigerian Journal of Botany 15, 37-41.

Aplin  THE,  Cannon  JR.  1971.  Distribution  of Alkaloids in some western Australian plants.Economic Botany 25(4), 366-380.

Bakrim A, Lamhamdi M, Sayah F, Chibi F.2007. Effects of plant hormones and 20-hydroxyecdysone on tomato (Lycopersicumesculentum) seed germination and seedling growth.African Journal of Biotechnology 6(24), 2792-2802.

Brault M, Maldiney R. 1999. Mechanisms of cytokinin action. Plant Physiology andBiochemistry 37, 403-412.

Chew YL, Goh JK, Lim YY. 2009. Assessment of in vitro antioxidant capacity and polyphenolic composition of selected medicinal herbs from Leguminosae family in Peninsular Malaysia. Food Chemistry 119, 373-378.

Clinton SK. 1998. Lycopene; Chemistry, biology and implications for human health and diseases. Nutrition Reviews56, 35-51.

Davies PJ. 1995. The plant hormone concept: concentration, sensitivity and transport. In: Davies, P.J. (ed.): Plant Hormones. Kluwer Academic Publishers, Dordrecht, Boston, London.pp. 13-38.

Doganlar S, Frary A, Tanksley SD. 2000. The genetic basis of seed-weight variation:tomato as a model system. Theoretical andApplied Genetics100, 1267-1273.

Edoga HO,Okwu DE, Mbaebie BO. 2005. Phytochemicals constituents of some Nigerian medicinal plants. African Journal ofBiotechnology 4(7), 685-688.

Ekwenye UN, Elegalam NN. 2005. Antibacterial activity of Ginger (Zingiberofficinale) Roscoe and Garlic (Allium sativum L.) extracts on Escherichia coli and Salmonella typhi. International Journal of Molecular Medicine and Advance Sciences 1(4), 411-416.

Farnsworth NR, Kinghorn AD, Soehart DD, Waller. 1985. Siberian Ginseng (Eleutheroccussenticosus): Current status as an adaptogen. In: Wagner H. Hikino H. and Farnsworth NR, (eds) In economic and Medicinal plant Research.Vol. 1 p.155-215. Academic press, Orlando, Florida, USA.

Fernandez-Ruiz V, Olives AI, Camara M, Sanchez-Mata MDE,Toriza ME. 2011. Mineral and trace elements content in 30 accessions of tomato fruits (Solanumlycopersicum L.) and wild relatives (Solanumpimpinellifolium L., Solanumcheesmaniae L. Riley and SolanumhabrochaitesS. Knapp and D.M. Spooner). Biological Trace Element Research 141, 329-339.

Finkelstein RR. 2004. Hormones in seed development and germination. In: Davies, P.J. (ed.). Plant hormones: Biosynthesis, signal transduction and action. Kluwer Academic Publishers. 513–537 p.

Foti MC. 2007. Antioxidant properties of phenols. Journal of Pharmacy and Pharmacology 59(12), 1673-85.

Frankel EN, Kanner J, German JB, Parks E, Kinsella JE. 1993. Inhibition of oxidation of human low density lipoprotein by phenolic substances in red wine. Lancet341, 454-457.

Frusciante L, Baroneb A, Carputob D, Ercolanoa MR, della Roccaa F, Espositoa S. 2000. Evaluation and use of plant diversity for food and pharmaceuticals.Fitoterapia 71, 66-72.

Gonçalves C, Dinis T, Batista MT. 2005. Antioxidant properties of proanthocyanidins of Uncariatomentosa bark decoction: a mechanism for anti-inflammatory activity. Phytochemistry 66, 89-98.

Goodwin JS, Brodwick M. 1995. Diet, aging and cancer. Clinics in Geriatric Medicine 11(4), 577-589.

Grassman J. 2005. Terpenoids as plant antioxidants. Vitamins & Hormones 72, 505-35.

Halliwell B. 1995. How to characterize an antioxidant- An update. Biochemical Society Symposium 61, 73-101.

Halliwell B. 1996. Antioxidants in human health and disease. Annual Reviews of Nutrition 16, 33-50.

Halliwell B, Gutteridge JMC. 1989. Free Radicals in Biology and Medicine. Clarendon Press, Oxford, UK.

Harborne JB. 1973. Phytochemicals Methods. Chapman and Hall Ltd., London, 49-188.

Hill AF. 1952. Economic Botany. A textbook of useful plants and plant products. 2nd edn. McGarw-Hill Book Company Inc, New York.

Hsu CY, Chan YP, Chang J. 2007. Antioxidant activity of extract from Polygonumcuspidatum. Biological Research 40, 13-21.

Kahkonen MP, Hopia AI, Vuorela HJ. 2008. Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agricultural and Food chemistry 47, 3954-3962.

Kaur C, Harish K. 2002. Antioxidant activity and total phenolic content of some Asian vegetables. International Journal of Food Science and Technology37, 153-161.

Koleva II, van Beek T, Linssen JPH, de Groot A, Evstatieva LN. 2002. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochemical Analysis 13, 8-17.

Kucera B, Cohn MA, Leubner-Metzger G. 2005. Plant hormone interaction during seed dormancy release and germination. Seed Science Research 15, 281-307.

Lasbrook CC, Tieman DM, Klee HJ. 1998. Differential regulation of the tomato ETR gene family throught plant development. Plant Journal15, 243-252.

Mandal P, Misra TK, Ghosal M. 2009. Free-radical scavenging activity and phytochemical analysis in the leaf and stem of DrymariadiandraBlume. International Journal of Integrative Biology 7(2), 80-84.

Morrissey PA, O’Brien NM. 1998. Dietary antioxidants in health and disease. International Dairy Journal 8, 463-472.

Nawaz A, Amjad M, Khan SM, Afzal I, Ahmed T, Iqbal Q, Iqbal J. 2013.Tomato seed invigoration with cytokinins. The Journal of Animal & Plant Sciences 23(1), 121-128.

Omodamiro OD, Amechi U. 2013. The phytochemical content, antioxidant, antimicrobial and anti-inflammatory activities of Lycopersiconesculentum (tomato). Asian Journal of Plant Science and Research 3(5), 70-81.

Pamplona-Roger D. 1998. Encyclopedia of Medicinal Plants, Saeliz, Spain.

Parnell TL, Suslow TV, Harris LJ. 2004. Tomatoes: Safe Methods to Store, Preserve, and Enjoy. ANR Catalog. University of California: Division of Agriculture and Natural Resources. Retrieved 18-02-2013.

Pavlov A, Kovatcheva P, Georgiev V, Koleva I. 2002. Biosynthesis and Radical Scavenging Activity of Betalains during the Cultivation of Red Beet (Beta vulgaris) Hairy Root Cultures. Zeitschrift fur Naturforschung 57, 640-644.

Philosoph-Hadas S, Friedman H, Meir S. 2005. Gravitropic Bending and Plant Hormones. Vitamins & Hormones 72, 31-78.

Pietta PG. 2000. Flavonoids as antioxidants. Journal of Natural Products 63(7), 1035-42.

Planta Medica. 2004. Antioxidant activity of saponins isolated from ivy: alpha-hederin, hederasaponin-C, hederacolchiside-E and hederacolchiside-F. 70(6), 561-3.

Plumb GW, Price KR, Williamson G. 1999. Antioxidant properties of flavonol glycosides from green beans. Redox Report 4(3), 123-7.

Sarla S, Prakash MA, Apeksha R, Subhash C. 2011. Free radical scavenging (DPPH) and ferric reducing ability (FRAP) of Aphanamixispolystachya (Wall) Parker. International Journal ofDrug Development and Research 3(4), 271-274.

Siddiqui AA, Ali M. 1997. Practical pharmaceutical chemistry. 1st Edition. CBS publishers and distributors New Delhi. 126-131.

Soares JR, Dins TCP, Cunha AP, Almeida LM. 1997. Antioxidant activity of some extracts of Thymus zygis. Free Radical Research26, 469-478.

Swaminathaan C, Srinivasan VM. 1996. Seedling invigoration through plant growth substances in teak (Tectonagrandis). Journal Tropical Forest Science 8, 310-316.

Trease GE, Evans WC. 1989. Pharmacognosy, 11thedn., BailliereTindall, London, 45-50.

Trigui M, Hsouna AB, Tounsi S, Jaoua S. 2013. Efficacy of Lawsoniainermis leaves extract and its phenolic compounds against olive knot and crown gall diseases. Industrial Crops and Products45, 83-88.

Walia S, Sing M, Kaur C, Kumar R, Joshi S. 2010. Antioxidant Composition of Red and Orange Cultivars of Tomatoes (SolanumlycopersiconL): A Comparative Evaluation. Journal of Plant Biochemistry and Biotechnology 19(1), 95-97.

Wall ME, Krider MM, Krewson CF, Eddy CR, Willaman JJ, Corell SS, Gentry HS. 1954. Steroidal sapogenins Survey of plants for steroidal sapogenins and other constituents. Journal of Pharmaceutical Sciences 1-7.

Wilcox JK,  Catignani  GL,  Lazarus  C.  2003. Tomatoes and cardiovascular health. Critical Reviews in Food Science and Nutrition 43(1), 1-18.

Yamaguchi T, Takamura H, Matoba T, Terao J. 1998. HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl. Bioscience Biotechnology and Biochemistry 62, 1201-1204.

Yu BP. 1994. Cellular defenses against damage from reactive oxygen species. Physiological Reviews 74(1), 139-162.

Zhang LJ, Yuan YJ, Wang CG, Pan XL. 2006. Physiological effects of NAA and SA on seed germination of Cornusofficinalis. Journal of Shaanxi Normal University Natural Science Edition 20(4), 75-79.


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