Study on the effect of colchicine on in-vitro cultures of turmeric (Curcuma longa L.) approach to polyploid development
Paper Details
Study on the effect of colchicine on in-vitro cultures of turmeric (Curcuma longa L.) approach to polyploid development
Abstract
Crop improvement possibilities in turmeric Curcuma longa (L.) are limited due to its triploid nature except for polyploidisation. Colchicine is a chemical that is used frequently to make plants polyploidy. Hence, this research aimed to study the effect of colchicine on the ability to induce polyploidisation of in-vitro turmeric cultures. The study consisted of two experiments: (1) assay on in-vitro culturing for callus induction and (2) assay on colchicine treatments and polyploidy screening. In experiment 1, turmeric rhizome buds were cultured on MS-solidified medium for callus induction with 100 mL coconut water, 2.5 and 4.5 mg L-1 2,4-D, 0.93 mg L-1 KIN, and without growth regulators. In addition, cell suspension culture was tested for callus induction with 4.5 mg L-1 2,4-D and 0.93 mg L-1 KIN. For polyploidy induction in experiment 2, in-vitro developed callus tissues were transferred to liquid MS medium supplemented with various concentrations of colchicine (0, 0.05, 0.10, 0.15, and 0.20%) for 2 days. Then acetocarmine staining method and microscopic observation were attempted to count the chromosome number. Nucleus size; nucleus area (µmSq) and perimeter (µm) were referred using microscopic observation under 1000× magnification and BEL capture software. The results revealed that MS-solidified medium supplemented with coconut water was most effective in inducing callus. The nucleus area (371.225 µmSq) and perimeter (65.725 µm) of the cells in 0.05% colchicine for 2 days showed the highest results. It can be concluded that 0.05% colchicine concentration has an effect on nuclear size increment thereby possibly inducing polyploidization of turmeric.
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Rathnayaka Mudiyanselage Nilan Suneth Dayarathna, Dandudeniye Gedara Harsha Manoj Kumara Dissanayake, Prathiba Aruni Weerasinghe, Randeniya Mudiyanselage Erandi Madushika Kumari Randeniya (2024), Study on the effect of colchicine on in-vitro cultures of turmeric (Curcuma longa L.) approach to polyploid development; IJAAR, V25, N2, August, P20-25
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