Sugar and anthocyanin characterization of four Iranian pomegranate (Punica granatum L.) varieties using HPLC System

Paper Details

Research Paper 01/04/2014
Views (495)
current_issue_feature_image
publication_file

Sugar and anthocyanin characterization of four Iranian pomegranate (Punica granatum L.) varieties using HPLC System

Somayeh Mirzaee
J. Biodiv. & Environ. Sci. 4(4), 248-253, April 2014.
Copyright Statement: Copyright 2014; The Author(s).
License: CC BY-NC 4.0

Abstract

Among all types of fruit, pomegranate has been known as an excellent fruit due to its high valued nutraceutical components; i.e. anthocyanins, phenolic compounds and organic acids. This research aimed to measure anthocyanins and sugars in different varieties of pomegranate. Four cultivars were Vahshe Kane Tehran, Gorche Shahvar Yazdi, Shirin Shahvare Yazd and Pust sefid. High performance liquid chromatography (HPLC) was used in order to analyze the samples for measuring their components. Cyanidin 3-glucoside, cyanidin 3,5-diglucoside, delphinidin 3-glucoside, pelargonidin 3-glucoside and pelargonidin 3,5-diglucoside were detected in all samples as major anthocyanins. However, their quantities were different such that cyanidin 3,5-diglucoside was the main pigment, followed by cyanidin 3-glucoside and delphinidin 3-glucoside, while pelargonidin derivatives were always present in small amounts. Pust Sefid and Shirin Shahvare Yazd cultivars contained more anthocyanin compared to Gorche Shahvar Yazdi and Vahshe Kane Tehran. Results showed Fructose, glucose and sucrose were major sugars of the cultivars. Vahshe Kane Tehran had the highest fructose and sucrose content, while Gorche Shahvar Yazdi had the highest glucose content. Sugar content in Gorche Shahvar Yazdi and Vahshe Kane Tehran was more than that in Pust sefid and Shirin Shahvare Yazd cultivars.

Mousavinejad G, Emam-Djomeh Z, Rezaei K, Khodaparast MHH. 2009. Identification and quantification of phenolic compounds and their effects on antioxidant activity in pomegranate juices of eight Iranian cultivars”, Journal of Food Chemistry 115, 1274–1278. http://dx.doi.org/10.1016/j.foodchem.2009.01.044

Gil MI, Tomas-Barberan FA, Hess-Pierce B, Holcroft DM, Kader AA. 2000. Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing”, Journal of Agricultural and Food Chemistry 48, 4581–4589. 10.1021/jf000404a

Du C, Wang P, Francis FJ. 1975. Anthocyanins of pomegranate, Punica granatum. Journal of Food Science 40, 417–418.

Hulme AC. 1970. The biochemistry of fruits and their products”, Vol. 1. London and New York: Academic Press.

Mertens-Talcott SU, Jilma-Stohlawetz P, Rios J, Hingorani L, Derendorf H. 2006. Absorption, metabolism and antioxidant effects of pomegranate (Punica granatum L.) Polyphenols after ingestion of a standardized extract in healthy human volunteers”, Journal of Agricultural and Food Chemistry 54, 8956–8961.

Kong JM, Chia LS, Goh NK, Chia TF, Brouillard R. 2003. Analysis and biological activities of anthocyanins. Journal of Phytochemistry 64, 923–933. http://dx.doi.org/10.1016/S0031-9422(03)00438-2

Miguel  G,  Fontes  C,  Antunes  D,  Neves  A, Martins  D.  2004.  Anthocyanin  concentration  of “Assaria”  pomegranate  fruits  during  different  cold storage conditions”, Journal of Biomedicine and Biotechnology 5, 338-342. http://dx.doi.org/10.1155/S1110724304403076

Sepúlveda E, Sáenz C, Peña Á, Robert P, Bartolomé B, Gómez-Cordovés C. 2010. Influence of the genotype on the anthocyanin composition, antioxidant capacity and color of Chilean pomegranate (Punica granatum L.) juices”, Chilean Journal of Agricultural Research 70(1), 50-57.

Melgarejo P, Salazar DM, Artes F. 2000. Organic acids and Sugars composition of harvested pomegranate fruits”. Eur Food Res Technol 211, 185-190. 10.1007/s002170050021

Fadavi A, Barzegar M, Azizi MH, Bayat M. 2005. Physicochemical composition of ten pomegranate cultivars (Punica granatum L.) grown in Iran”, Food Science and Technology International 11, 113-119. 10.1177/1082013205052765

Related Articles

Overemphasis on blue carbon leads to biodiversity loss: A case study on subsidence coastal wetlands in southwest Taiwan

Yih-Tsong Ueng, Feng-Jiau Lin, Ya-Wen Hsiao, Perng-Sheng Chen, Hsiao-Yun Chang, J. Biodiv. & Environ. Sci. 27(2), 46-57, August 2025.

An assessment of the current scenario of biodiversity in Ghana in the context of climate change

Patrick Aaniamenga Bowan, Francis Tuuli Gamuo Junior, J. Biodiv. & Environ. Sci. 27(2), 35-45, August 2025.

Entomofaunal diversity in cowpea [Vigna unguiculata (L.) Walp.] cultivation systems within the cotton-growing zone of central Benin

Lionel Zadji, Roland Bocco, Mohamed Yaya, Abdou-Abou-Bakari Lassissi, Raphael Okounou Toko, J. Biodiv. & Environ. Sci. 27(2), 21-34, August 2025.

Biogenic fabrication of biochar-functionalized iron oxide nanoparticles using Miscanthus sinensis for oxytetracycline removal and toxicological assessment

Meenakshi Sundaram Sharmila, Gurusamy, Annadurai, J. Biodiv. & Environ. Sci. 27(2), 10-20, August 2025.

Bacteriological analysis of selected fishes sold in wet markets in Tuguegarao city, Cagayan, Philippines

Lara Melissa G. Luis, Jay Andrea Vea D. Israel, Dorina D. Sabatin, Gina M. Zamora, Julius T. Capili, J. Biodiv. & Environ. Sci. 27(2), 1-9, August 2025.

Effect of different substrates on the domestication of Saba comorensis (Bojer) Pichon (Apocynaceae), a spontaneous plant used in agroforestry system

Claude Bernard Aké*1, Bi Irié Honoré Ta2, Adjo Annie Yvette Assalé1, Yao Sadaiou Sabas Barima1, J. Biodiv. & Environ. Sci. 27(1), 90-96, July 2025.

Determinants of tree resource consumption around Mont Sangbé national park in western Côte d’Ivoire

Kouamé Christophe Koffi, Serge Cherry Piba, Kouakou Hilaire Bohoussou, Naomie Ouffoue, Alex Beda, J. Biodiv. & Environ. Sci. 27(1), 71-81, July 2025.