Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | October 1, 2015

| Download 1

The effect of packaging and storage of vitamin-C in fresh and commercial orange juices

Afshan Kaleem, Asma Tanveer, Irfana Iqbal, Moneeza Abbas, Roheena Abdullah, Mehwish Iqtedar, Mahwish Aftab, Tehreema Iftikhar, Shagufta Naz

Key Words:

Int. J. Biosci.7(4), 22-30, October 2015

DOI: http://dx.doi.org/10.12692/ijb/7.4.22-30


IJB 2015 [Generate Certificate]


Vitamin-C in commercial and fresh orange juice samples was investigated. The commercially available juices included packed and powdered orange juices. Different brands of commercial liquid and powdered orange juices were included in this study. The seasonal variation of vitamin-C in fresh oranges was also explored. Fresh cultivar of oranges including Ambersweet, Tangarin and Mandrin were selected for vitamin-C content. Furthermore the effect of storage, packing material lined and unlined with different fabrics and preservatives on the stability of vitamin-C in orange juice samples were investigated. Redox titration was used for measurement of vitamin-C content in all samples. Maximum vitamin-C content was observed in end of the season fresh juice samples. Some of the commercially available orange juices (within expiry date) showed highest vitamin-C content, while mostly showed loss of vitamin-C during preservation process.


Copyright © 2015
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

The effect of packaging and storage of vitamin-C in fresh and commercial orange juices

Andrews FE, Driscoll PJ. 1977. Stability of ascorbic acid in orange juice exposed to light and air during storage. Journal of the American Dietetic Association 71, 140-142.

Aruoma OI. 1998. Free radicals, oxidative stress and antioxidants in human health and disease. Journal of the American Oil Chemists’ Society 75, 199-212.

Bacigalupi C, Lemaistre MH, Boutroy N, Bunel C, Peyron S, Guillard V, Chalier P. 2013. Changes in nutritional and sensory properties of orange juice packed in PET bottles: an experimental and modelling approach. Food Chemistry 141, 3827-3836.

Bolling BW, Chen YY, Chen CY. 2013. Contributions of phenolics and added vitamin-C to the antioxidant capacity of pomegranate and grape juices: synergism and antagonism among constituents. International Journal of Food Science & Technology 48, 2650–2658.

Bossi A, Piletsky SA, Piletska EV, Righetti PG, Turner AP. 2000. An assay for ascorbic acid based on polyaniline-coated microplates. Analytical Chemistry 72, 4296-4300.

Brenes CH, DelPozo-Insfran D, Talcott ST. 2005. Stability of copigmented anthocyanins and ascorbic acid in a grape juice model system. Journal of Agricultural and Food Chemistry 53, 49‒56.

Daglia M, Papetti A, Gregotti C, Berte F, Gazzani G. (2000). In vitro antioxidant and ex vivo protective activities of green and roasted coffee. Journal of Agricultural and Food Chemistry 48, 1449-1454.

Davalos A, Bartolome B, Gomez-Cordoves C. 2005. Antioxidant properties of commercial grape juices and vinegars. Food Chemistry 93, 325-330.

Djordjevic B, Savikin K, Zdunic G, Jankovic T, Vulic T, Pljevljakusic D, Oparnica C. 2013. Biochemical properties of the fresh and frozen black currants and juices. Journal of Medicinal Food 16, 73-81.

Doolaege EH, Vossen E, Raes K, De Meulenaer B, Verhe R, Paelinck H, De Smet S. 2012. Effect of rosemary extract dose on lipid oxidation, colour stability and antioxidant concentrations, in reduced nitrite liver pates. Meat Science 90, 925‒931.

Gillman MW, Cupples LA, Gagnon D, Posner BM, Ellison RC, Castelli WP, Wolf PA. 1995. Protective effect of fruits and vegetables on development of stroke in men. The Journal of the American Medical Association 273, 1113-1117.

Janjarasskul T, Min SC, Krochta JM. 2011. Storage stability of ascorbic acid incorporated in edible whey protein films. Journal of the Science of Food and Agriculture 59, 12428‒12432.

Juhasz M, Kitahara Y, Takahashi S, Fujii T. 2012. Thermal stability of vitamin C: thermogravimetric analysis and use of total ion monitoring chromatograms. Journal of Pharmaceutical and Biomedical Analysis 59, 190-193.

Justi KC, Visentainer JV, Evelazio de Souza N, Matsushita M. 2000. Nutritional composition and vitamin-C stability in stored camu-camu (Myrciaria dubia) pulp. Archivos Latinoamericanos de Nutrición 50, 405-408.

Kahkonen MP, Hoia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS, Heinonen M. 1999. Antioxidant activity of plant extracts containing phenolic compounds. Journal of the Science of Food and Agriculture 47, 3954‒3962.

Koh E, Charoenprasert S, Mitchell AE. 2012. Effects of industrial tomato paste processing on ascorbic acid, flavonoids and carotenoids and their stability over one-year storage. Journal of the Science of Food and Agriculture 92, 23-28.

Kong Y, Shan X, Ma J, Che M, Chen Z. 2014. A novel voltammetric sensor for ascorbic acid based on molecularly imprinted poly(o-phenylenediamine-co-o-aminophenol). Analytica Chimica Acta 809, 54-60.

La   Vecchia    C,    Altieri   A,    Tavani    A.    2001. Vegetables, fruit, antioxidants and cancer: a review of Italian studies. European Journal of Nutrition 40, 261-267.

Lavelli V, Corey M, Kerr W, Vantaggi C. 2011. Stability and anti-glycation properties of intermediate moisture apple products fortified with green tea. Food Chemistry 127, 589-595.

Licciardello F, Muratore G. 2011. Effect of temperature and some added compounds on the stability of blood orange marmalade. Journal of Food Science 76, C1094‒1100.

Llorent-Martinez EJ, Molina-Garcia L, Kwiatkowski R, Ruiz-Medina A. 2013. Application of quantum dots in clinical and alimentary fields using multicommutated flow injection analysis. Talanta 109, 203-208.

Muszalska I, Zajac M, Wrobel G, Nogowska M. 2000. Redox methods validation of paracetamol and ascorbic acid in pharmaceutical preparations. Acta Poloniae Pharmaceutica 57, 27‒32.

Proteggente AR, Pannala AS, Paganga G, Va BL, Wagner E, Wisema S, Vande PF, Dacombe C, Rice-Evans CA. 2002. The antioxidant activity of regular consumed fruit and vegetables reflects their phenolic and vitamin-C composition. Free Radical Research 36, 217-233.

Ramadan MF, Kroh LW, Moersel JT. 2003. Radical scavenging activity of black cumin (Nigella sativa L.),(Guizotia abyssinica Cass.) crude seed oils and oil fractions. Journal of the Science of Food and Agriculture 51, 6961‒6969.

Ramadan MF, Moersel JT. 2007. Impact of enzymatic treatment on chemical composition, physicochemical properties and radical scavenging activity of goldenberry (Physalis peruviana L.) juice. Journal of the Science of Food and Agriculture 87, 452-460.

Ramaiya SD, Bujang JS, Zakaria MJ, King WS, Shaffiq Sahrir MA. 2013. Sugars, ascorbic acid, total phenolic content and total antioxidant activity in passion fruit (Passiflora) cultivars. Journal of the Science of Food and Agriculture 93, 11981205.

Ribeiro DO, Pinto DC, Lima LM, Volpato NM, Cabral LM, de Sousa VP. 2011. Chemical stability study of vitamins thiamine, riboflavin, pyridoxine and ascorbic acid in parenteral nutrition for neonatal use. Nutrition Journal 10, 47.

Richelle M, Travazzi I, Offord E. 2001. Comparison of the antioxidant activity of commonly consumed polyphenolic beverages (coffee, cocoa and tea) prepared per cup serving. Journal of Agricultural and Food Chemistry 49, 3438-3442.

Riemersma RA, Carruthers KF, Elton RA, Fox KA. 2000. Vitamin-C and the risk of acute myocardial infarction. The American Journal of Clinical Nutrition 71, 1181-1186.

Rodríguez-Bernaldo de Quirós A, Costa HS. 2006 Analysis of carotenoids in vegetable and plasma samples: A review. Journal of Food Composition and Analysis 19, 97-111.

Salama MF, Abbas A, Darweish MM, El-Hawwary AA, Al-Gayyar MM. 2013. Hepatoprotective effects of cod liver oil against sodium nitrite toxicity in rats. Pharmaceutical Biology 51, 1435-1443.

Sargeant LA, Jaeckel A, Wareham NJ. 2000. Interaction of vitamin-C with the relation between smoking and obstructive airways disease in EPIC Norfolk. European Prospective Investigation into Cancer and Nutrition. European Respiratory Journal 16, 397-403.

Shinn C, Marco A, Serrano L. 2013. Influence of low levels of water salinity on toxicity of nitrite to anuran larvae. Chemosphere 92, 1154-1160.

Shyla B, Nagendrappa G. 2013. Redox spectrophotometric method involving electrolytically generated manganese(III) sulphate with diphenylamine for the determination of ascorbic acid present in the samples of various fruits, commercial juices and sprouted food grains. Food Chemistry 138, 2036-2042.

Sigusch BW. 2013. The role of vitamin-C (ascorbic acid) in the prevention and therapy of oral diseases. Archives of Oral Biology 58, 905-906.

Sorice A, Guerriero E, Capon, F, Colonna G, Castello G, Costantini S. 2014. Ascorbic acid: its role in immune system and chronic inflammation diseases. Mini Reviews in Medicinal Chemistry 14, 444-452.

Stander MA, Kuhn W, Hiten NF. 2013. Survey of South African fruit juices using a fast screening HILIC-MS method. Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment 30, 1473-1484.

Terry P, Terry JB, Wolk A. 2001. Fruit and vegetable consumption in the prevention of cancer: an update. Journal of General Internal Medicine 250, 280-290.

Valente A, Sanches-Silva A, AlbuquerqueTG, Costa HS. 2014. Development of an orange juice in-house reference material and its application to guarantee the quality of vitamin-C determination in fruits, juices and fruit pulps. Food Chemistry 154, 71-77.

Verdini RA, Lagier CM. 2000. Voltammetric iodometric titration of ascorbic acid with dead-stop end-point detection in fresh vegetables and fruit samples. Journal of the Science of Food and Agriculture 48, 2812-2817.

Willett WC. 1991. Micronutrients and cancer risk. The Journal of the American Medical Association 53, 265S-269S.

Yokoyama T, Date C, Kokubo Y, Yoshiike N, Matsumura Y, Tanaka H. 2000. Serum vitamin-C concentration was inversely associated with subsequent 20‒year incidence of stroke in a Japanese rural community. The Shibata study. Stroke 31, 2287-2294.


Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background