Total phenolic contents of two varieties of Crocus sativus and their antioxidant activity

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Research Paper 01/03/2019
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Total phenolic contents of two varieties of Crocus sativus and their antioxidant activity

Zafar Iqbal, Mohsan Javed, Samina Gull, Mian Habib-Ur-Rahman Mahmood, Zhang Hai
Int. J. Biosci.14( 3), 128-132, March 2019.
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Methanolic extract of two varieties of crocus sativus dried stigmas was taken for total phenolic contents and antioxidant activity by using 1,1-diphenyl-2-picryl hydroxide scavenging. UV absorption of both samples separately showed three maximum wavelengths 236 nm and 234 nm (maximum absorbance of picrococine), 320 nm and 308 nm (maximum absorbance of safranal) and 436 nm and 434 nm (maximum absorbance of crocine) respectively. The electrochemical studies showed the oxidation reduction peaks. Total Phenolic content of red and brown saffron by using FC reagent was 5.05 mg and 4 mg of gallic acid equivalent/g of saffron extract respectively. At 50 µL concentration, both red and brown crocus sativus showed 71.68% and 73.70% inhibition respectively.


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