Toxic and teratogenic effects of mycelia and fruiting body extracts of Lentinus strigosus (BIL 1324) in zebra fish (Danio rerio) embryo
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Toxic and teratogenic effects of mycelia and fruiting body extracts of Lentinus strigosus (BIL 1324) in zebra fish (Danio rerio) embryo
Abstract
We reported in the previous works the successful rescue, domestication, optimization, enriched cultivation, compositions, and functional activities of Lentinus strigosus, a naturally occurring, wood-rotting edible mushroom. Herein, the embryo-toxic and teratogenic effects of water extracts of mycelia and fruiting body of this mushroom in zebra fish (Danio rerio) embryos were investigated. The toxic effects of both extracts were dependent on concentration and time of exposure. Embryos at 1000 µg/ml (after 24 hrs) and 100 µg/ml (after 48 hrs) and lower concentrations of mycelial extract survived while 100% mortality was recorded to those exposed at 10000 µg/ml (after 24 hrs) and 1000 µg/ml (after 48 hrs) concentration of fruiting body extract. Both extracts significantly reduced the hatchability and heartbeat rate of zebra fish. Delayed development was the sole teratogenic effect and this was obvious to embryos exposed at 100 µg/ml of both mycelia and fruiting body extracts. Therefore, L. strigosus holds promising potential as source of teratogens, which are also known as anticancer agents.
Blagosklonny M. 2005. Teratogens as anticancer drugs. Cell Cycle 4(11), 1518–21.
Busquet F, Nagel R, Landenberg F, Mueller S, Huebler N, Broschard T. 2008. Development of a new screening assay to identify proteratogenic substances using zebra fish Danio rerio embryo combined with an exogenous mammalian metabolic activation system (mDarT). Toxicological Sciences 104(1), 177–188.
Bustillos RG, Paguio ZKG, Hermosa DP, Dulay RMR. 2016. Philippine coprophilous mushrooms (Panaeolus antillarium and Panaeolus cyanescens) exhibit toxic and teratogenic effects in zebra fish (Danio rerio) embryo model. Advances in Environmental Biology 10(3), 75-80.
De Castro MEG, Dulay RMR, Enriquez MLD. 2016. Toxic and Teratogenic Effects of Medicinal and Culinary Mushroom, Termitomyces clypeatus, Collected from the Termite Mound in Mt. Makiling Forest Reserve, Los Baños, Laguna, Philippines on Developing Embryos of Zebra fish (Danio rerio). Der Pharmacia Lettre 8(5), 237-242.
De Castro MEG, Dulay RMR. 2015. Toxic and teratogenic effects of Lentinus sajor-caju and Pleurotus ostreatus ethanolic extracts in Danio rerio embryo model. International Journal of Biology, Pharmacy and Allied Sciences 4(4), 2261-2269.
Dulay RMR, Garcia EJB. 2017. Optimization and enriched cultivation of Philippine (CLSU) strain of Lentinus strigosus (BIL1324). Biocatalysis and Agricultural Biotechnology 12, 323-328.
Dulay RMR, Kalaw SP, Reyes RG, Alfonso NF, Eguchi F. 2012. Teratogenic and toxic effects of lingzhi or reishi medicinal mushroom, Ganoderma lucidum (W.Curt.:Fr.) P. Karst. (Higher basidiomycetes), on zebrafish embryo as model. International Journal of Medicinal Mushrooms 14(5), 507–512.
Dulay RMR, Kalaw SP, Reyes RG, Cabrera EC. 2014. Embryo-toxic and teratogenic effects of Philippine strain of Lentinus tigrinus (tiger sawgill basidiomycetes) extract on zebra fish (Danio rerio) embryos. Annals of Biological Research 5(6), 9–14.
Dulay RMR, Pamiloza DG. 2018. Proximate composition and bioactivities of hairy sawgill mushroom, Lentinus strigosus (BIL 1324) from the Philippines. International Journal of Biology, Pharmacy and Allied Sciences 7(3), 361-369.
Dulay RMR, Rivera AGC, Garcia EJB. 2017. Mycelial growth and basidiocarp production of wild hairy sawgill Lentinus strigosus, a new record of naturally occurring mushroom in the Philippines. Biocatalysis and Agricultural Biotechnology 10, 242-246.
Nagel R. 2002. DarT: The embryo test with the Zebra fish Danio rerio–a general model in ecotoxicology and toxicology. ALTEX 19(1), 38-48.
Souza-Fagundes EM, Cota BB, Rosa LH, Romanha AJ, Correa-Oliveira R, Rosa CA, Zani CL, Teixeira-Carvalho A, Martins-Filho OA. 2010. In vitro activity of hypnophilin from Lentinus strigosus: a potential prototype for Chagas disease and leishmaniasis chemotherapy. Brazillian Journal of Medical and Biological Research 43, 1054-1061.
Thomas J. 2000. The Zebra fish Book 4th edition, University of Oregon.
Rich Milton R. Dulay, Danielle G. Pamiloza, Rolen L. Ramirez, 2018. Toxic and teratogenic effects of mycelia and fruiting body extracts of Lentinus strigosus (BIL 1324) in zebra fish (Danio rerio) embryo. Int. J. Biosci., 13(5), 205-211.
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