Viability formulation with active Trichoderma asperellum TR3 in three packaging variation
Paper Details
Viability formulation with active Trichoderma asperellum TR3 in three packaging variation
Abstract
Trichoderma asperellum TR3 has been applied as a biopesticide and Plant Growth Promotion Fungi (PGPF) but in the form of a suspension and substrate without the addition of carriers and adhesives so for long-term use and short shelf life. This study aims to obtain the right packaging formulation and have good viability, as well as a long storage time using talc and tapioca flour as the basic ingredients. The isolate collection was rejuvenated by growing in PDA medium, after completion of incubation for 48 hours, the growing fungal colonies were counted, isolated and purified, then made in the form of a powder starter. Test of various formulations of T. asperellum TR3 fungal powder packaging was carried out by viability test using a Completely Randomized Design (CRD), with packaging treatment on the formulation: P1 = Aluminum foil plastic, P2 = Bottle and P3 = Cetic plastic. Each treatment was repeated 3 times to obtain a combination of 9 treatment units. Furthermore, all packaging combinations were stored at room temperature. In the first month, 1 pack was taken for regrowth which aims to determine the viability of the conidia and then every two weeks for 12 weeks. The results showed that the average number of conidia colonies growing on various packages gave different viability results, but had no significant effect between formulations of various packages and viability. The average number of colonies that grew on aluminum foil plastic packaging was more in each observation of storage time compared to bottle and plastic packaging.
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Ratnawati Ratnawati, Arfan Arfan, Kasman Jaya, Sri Sudewi, Mr. Mufida (2022), Viability formulation with active Trichoderma asperellum TR3 in three packaging variation; IJMM, V15, N4, October, P26-31
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