Comparative analysis of phenolic compounds in two samples of Rosa damascena by HPLC

Paper Details

Research Paper 01/07/2015
Views (434) Download (28)
current_issue_feature_image
publication_file

Comparative analysis of phenolic compounds in two samples of Rosa damascena by HPLC

Zahra Memariani, Gholamreza Amin, Ghazaleh Moghaddam, Mannan Hajimahmoodi
Int. J. Biosci.7( 1), 112-118, July 2015.
Certificate: IJB 2015 [Generate Certificate]

Abstract

Rosa damascena Mill. (Rosaceae) commonly known as Damask rose is widely grown in Kashan and Tabriz of Iran which has different therapeutic indications among Iranian traditional medicine practitioners. R. damascena is a rich source of phenolic compounds responsible for its medicinal properties. The objective of this study was to quantify the phenolic compounds present in R. damascena samples. The analytical separation and determination of phenolic compounds were performed using reversed phase HPLC with UV detector. Based on the results obtained throughout this study total phenolic and total flavonoid contents of sample from Tabriz (217.728 ± 0.13 and 22.8 ± 0.18 mg CE/g, respectively) were significantly higher than Kashan (134.568 ± 0.11 and 15.84 ± 0.23 mg CE/g respectively). Also phenolic compounds including gallic acid, syringic acid and quercetin were detected in both samples. The most abundant phenolic compound was gallic acid with amounts of 118.213 mg/g in Tabriz and 86.562 mg/g in Kashan samples (p < 0.05) but there was not any significant difference between two samples in the case of quercetin and syringic acid. The present study represents differences in total phenolics and gallic acid between two studied samples which might be implicated in different antioxidant activity and therapeutic applications of R. damascena from Tabriz and Kashan.

VIEWS 43

Abbasian S, Karimi F, Moghaddam G, Soroush A, MoloudianH, Ahosseini MA. 2013. Antioxidant properties of different black tea samples and some Iranian native plants. International Journal of Comprehensive Pharmacy 4, 1–5.

Abdel-Hameed ES, Bazaid SA, Shohayeb MM. 2012. Total phenolics and antioxidant activity of defatted fresh Taif rose Saudi Arabia. British Journal of Pharmaceutical Research 2, 129-140.

Achuthan CR, Babu BH, Padikkala J. 2003. Antioxidant and hepatoprotective effects of Rosa damascena. Pharmaceutical Biology 41, 357-361.

Baydar NG, Baydar H. 2013. Phenolic compounds, antiradical activity and antioxidant capacity of oil-bearing rose (Rosa damascena Mill.) extracts. Industrial Crops and Products 41, 375-380. http://dx.doi.org/10.1016/j.indcrop.2012.04.045

Baziar S, Zakerin A, Rowshan V. 2013. Antioxidant properties and polyphenolic compounds of Rosa damascena collected from different altitudes. International Journal of Agronomy and Plant Production 4, 2937-2942.

Boskabady MH, Shafei MN, Saberi Z, Amini S. 2011. Pharmacological effects of Rosa damascena. Iranian Journal of Basic Medical Sciences 14, 295-307.

Cai Y, Luo Q, Sun M, Corke H. 2004. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sciences 74, 2157-2184. http://dx.doi.org/10.1016/j.lfs.2003.09.047

Farzaei MH, Khanav M, Moghaddam G, Dolatshahi F, Rahimi R, Shams-Ardekani MR, Amin G, Hajimahmoodi M. 2014. Standardization of Tragopogon graminifolius DC. extract based on phenolic compounds and antioxidant activity. Journal of Chemistry 2014, 1-5. http://dx.doi.org/10.1155/2014/425965

Ginova A, Kiril M, Violeta K. 2013. Antioxidant capacity of petals and leaves from different rose (Rosa damascena Mill.) plantations in Bulgaria. International Journal of Pure and Applied Bioscience 1, 38-43.

Haghi G, Hatami A. 2010. Simultaneous quantification  of  flavonoids  and  phenolic  acids  in plant materials by a newly developed isocratic high-performance liquid chromatography approach. Journal of Agricultural and Food Chemistry 58, 10812-10816. http://dx.doi.org/10.1021/jf102175x

Hajimahmoodi M, Faramarzi MA, Mohammadi N, Soltani N, Oveisi MR, Nafissi-Varcheh N. 2010. Evaluation of antioxidant properties and total phenolic contents of some strains of microalgae. Journal of Applied Phycology 22, 43–50. http://dx.doi.org/10.1007/s10811-009-9424-y

Hajimahmoodi M, Moghaddam G, Ranjbar AM, Khazani H, Sadeghi N, Oveisi MR, Jannat B. 2013. Total phenolic, flavonoids, tannin content and antioxidant power of some Iranian pomegranate flower cultivars (Punica granatum L.). American Journal of Plant Sciences 4, 1815-1820. http://dx.doi.org/10.4236/ajps.2013.49223

IbnSina H. 2005. Al-Qanon fi al-Tibb. Alamy Le- Al-Matbooat institute. Beirut.

Kim HY, Kim OH, Sung MK. 2003. Effects of phenol-depleted and phenol-rich diets on blood markers of oxidative stress, and urinary excretion of quercetin and kaempferol in healthy volunteers. Journal of the American College of Nutrition 22, 217-223.

Kumar N, Bhandari P, Singh B, Bari SS. 2009. Antioxidant activity and ultra-performance LC-electrospray ionization-quadrupole time-of-flight mass spectrometry for phenolics-based fingerprinting of Rose species: Rosa damascena, Rosa bourboniana and Rosa brunonii. Food Chemistry Toxicology 47, 361-367. http://dx.doi.org/10.1016/j.fct.2008.11.036

Kumar N, Bhandari P, Singh B, Gupta AP, Kaul VK. 2008. Reversed phase‐HPLC for rapid determination of polyphenols in flowers of rose species. Journal of Separation Science 31, 262-267. http://dx.doi.org/10.1002/jssc.200700372

Moein S, Moein M, Khoshnoud MJ, Kalanteri T. 2012. In vitro antioxidant properties evaluation of 10 Iranian medicinal plants by different methods. Iranian Red Crescent Medical Journal 14, 771-775. http://dx.doi.org/10.5812/ircmj.1408

Naithani V, Nair S, Kakkar P. 2006. Decline in antioxidant capacity of Indian herbal teas during storage and its relation to phenolic content. Food Research International 39, 176-181. doi:10.1016/j.foodres.2005.07.004

Özkan G, Sagdic O, Baydar N, Baydar H. 2004. Note: Antioxidant and antibacterial activities of Rosa damascena flower extracts. Food Science and Technology International 10, 277-281. http://dx.doi.org/10.1177/1082013204045882

Schiber A, Mihalev K, Berardini N, Mollov P, Carle R. 2005. Flavonol glycosides from distilled petals of Rosa damascena Mill. Zeitschrift für Naturforschung C 60, 379-384.

Shahriari S, Yasa N, Mohammadirad A, Khorasani R, Abdollahi M. 2007. In vivo antioxidant potentials of Rosa damascena petal extract from Guilan, Iran, comparable to a-tocopherol. International Journal of Pharmacology 3, 187-190. http://dx.doi.org/10.3923/ijp.2007.187.190

Sharafi SM, Rasooli I, Dehghan Kashani A, Owlia P, Rezaee MB, Darvish Alipoor Astaneh S. 2010. Cytobiochemical Potentials of Rosa damascena Mill. Extract. Iranian Journal of Pathology 5, 184-193.

Velioglu YS, Mazza G. 1991. Characterization of flavonoids in petals of Rosa damascena by HPLC and spectral  analysis.  Journal  of  agricultural  and  food chemistry 39, 463-467. http://dx.doi.org/10.1021/jf00003a007

Vinokur Y, Rodov V, Reznick N, Goldman G, Horev B, Umiel N, Friedman H. 2006. Rose Petal Tea as an Antioxidant‐rich Beverage: Cultivar Effects. Journal of Food Science 71, S42-S47. http://dx.doi.org/10.1111/j.13652621.2006.tb12404.x

Yassa N, Masoomi F, Rankouhi SR, Hadjiakhoondi A. 2009. Chemical composition and antioxidant activity of the extract and essential oil of Rosa damascena from Iran, population of Guilan. DARU Journal of Pharmaceutical Sciences 17, 175-180.

Zhishen J, Mengcheng T, Jianming W. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry 64, 555–559. http://dx.doi.org/10.1016/S0308-8146(98)00102-2