Antimicrobial properties of the extracts of locally sold garlic and neem leaf in Benin City, Nigeria

Paper Details

Research Paper 01/04/2012
Views (781)
current_issue_feature_image
publication_file

Antimicrobial properties of the extracts of locally sold garlic and neem leaf in Benin City, Nigeria

Irorere Uhunoma Victor, Igeleke C. L
Int. J. Biosci. 2(4), 21-27, April 2012.
Copyright Statement: Copyright 2012; The Author(s).
License: CC BY-NC 4.0

Abstract

Different varieties of medicinal plants have been reported and a recent study on various herbal plants sold in Benin City, Nigeria has revealed the presence of disease causing organisms present on them. This report questions the antimicrobial potency of these locally sold varieties of medicinal plants. In this study, we obtained garlic and neem leaf from local herbal practitioners in Benin City, Nigeria and prepared three different extracts (methanol, acetone and chloroform) of both samples. We then used the disc diffusion method of antimicrobial assay to analyse the antimicrobial properties of these extracts against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi. Our results showed that at least two of the test organisms are susceptible to each garlicextract, with the chloroform extract having the most antimicrobial property against all microorganisms at concentration as low as 2.26mg/ml. The extracts of neem leaf however showed antimicrobial property against S. aureus and E. coli only with the acetone extract having the highest antimicrobial potency against these two organismsat a concentration of 6.84mg/ml. These results thus revealed the antimicrobial property of locally sold garlic and neem leaf for herbal medicines. However, it is suggested that these plants be thoroughly washed with water before use to reduce or eliminate any microbial contamination due to field contamination.

Abere T. A., Onyekweli A. O. and Ukoh G. C. 2007. Invitro antimicrobial activity of the extracts of Miltracarpus scaber leaves formulated as syrup. Tropical Journal of Pharmaceutical Research 6(1), 671– 682.

Bakht J, Tayyab M, Ali H, Islam A, Shafi M. 2011. Effect of different solvent extracted samples of Allium sativum (Linn) on bacteria and fungi. African Journal of Biotechnology 10(31), 5910 – 5915.

Bala M, Ray K, Gupta SM. 2005. Comparison of disc diffusion results with minimum inhibitory concentration (MIC) values for antimicrobial susceptibility testing of Neisseria gonorrhoeae. Indian Journal of Medical Research 122, 48 – 51.

Bisht R, Katiyar A, Singh R, Mittal P. 2009. Antibiotic Resistance: An issue of Global Concern. Asian Journal of Pharmaceutical and Clinical Research 2(2), 34 – 39.

Biswas K, Chattopadhyay I, Barnerjee KR, Bandyopadhyay U. 2002. Biological activities and medicinal properties of neem (Azadirachta indica). Current Science 82(11), 1336 – 1345.

Daka D. 2011. Antibacterial effect of garlic (Allium sativum) on Staphyloccus aureus: An in vitro study. African Journal of Biotechnology 10(4), 666 – 669.

Durairaj S, Srinivasan S, Lakshmanaperumalsamy P. 2009. In vitro Antibacterial Activity and Stability of Garlic Extract at Different pH and Temperature. Electronic Journal of Biology 5(1), 5 – 10.

Harunobo A. 2006. Clarifying the Real Bioactive Component of Garlic. Journal of Nutrition 136, 716 – 725.

Idu M, Erhabor JO, Idele SO. 2011. Microbial Load of Some Medicinal Plants Sold in Local Market in Benin City Nigeria. International Journal of Medical and Aromatic Plants 1(3), 272 – 277.

Joshi B, Sah PG., Basnet BB., Bhatt MR, Sharma D, Subedi KPJ, Malla R. 2011. Phytochemical extraction and antimicrobial properties of different medicinal plants: Ocimum sanctum (Tulsi), Eugenia caryophyllata (Clove), Achyranthes bidentata (Datiwan) and Azadirachta indica (Neem). Journal of Microbiology and Antimicrobials 3(1), 1 – 7.

Koul A, Bharrhan S, Singh B, Rishi P. 2008. Potential of Azadirachta indica against Salmonella typhimurium – induced inflammation in BALB. Inflammopharmacology 17(1), 29 – 36.

Okigbo R. N. and Mmeka E. C. 2006. An appraisal of phytomedicines in Africa. KMITL Science and Technology Journal 6(2), 83 – 94.

Okonko IO, Fajobi EA, Ogunnusi TA, Ogunjobi AA, Obiagbolo CH. 2008. Antimicrobial chemotherapy and Sustainable Development: The past, The Current Trend, and the future. African Journal of Biomedical Research 11, 235 – 250.

Onyeagba RA, Ugbogu OC, Okeke CU, Iroakasi O. 2004. Studies on the antimicrobial effects of garlic (Allium sativum                Linn), ginger (Zingiber officinale Roscoe) and lime (Citrus aurantifolia Linn). African Journal of Biotechnology 3(10), 552 – 554.

Pandy M, Debnath M, Gupta S, Shikara SK. 2011. Phytomedicine: An ancient approach turning into future potential source of therapeutics. Journal of Pharmacognosy and phytotherapy 3(3), 27 – 37.

Shobana S, Vidhya VG, Ramya M. 2009. Antibacterial Activity of Garlic Varieties (Ophioscordon and Sativum) on Enteric Pathogens. Current Research Journal of Biological Sciences 1(3), 123 – 126.

Turkmen N, Velioglu SY, Sari F, Polat G. 2007. Effect of Extraction Conditions on Measured Total Polyphenol Contents and Antioxidant and Antibacterial Activities of Black Tea. Molecules 12, 484– 496.

Tiwara P, Kumar B, Kaur M, Kaur G, Kaur H. 2011. Phytochemical Screening and Extraction: A Review. Internationale Pharmaceutica.

Related Articles

The protective effect of black pepper (Piper nigrum) on liver enzymes in streptozotocin-induced diabetic rats

Amani A. R. Filimban, Khulud A. Wathi, Int. J. Biosci. 27(4), 140-149, October 2025.

Production of bioplastics (PHB) using waste paper as feed stock by Cupriavidus taiwanensis

Ajeena Davis, Jini Joseph, Int. J. Biosci. 27(4), 130-139, October 2025.

Gill ectoparasites of the mugilidae from the Ebrié lagoon, Abidjan (Côte d’Ivoire)

Eby Yoboué Gnamma Honorine Alla, Carel Wilfried Bermian Dibi-Ahui, Fidèle Kouassi Kouakou, Abouo Béatrice Adepo-Gourene, Int. J. Biosci. 27(4), 123-129, October 2025.

Impacts of diverse water management systems on growth and yield of two prominent boro rice cultivars in Bangladesh

Zahidul Islam, Md Ekhlasur Rahman, Md Khayrul Islam Bashar, Sharmin Sultana, Md Taharat Al Tauhid, Md Rabiul Islam, Md Shahed Hossain, Md Musa Mondal, Pradip Kumar Biswas, Int. J. Biosci. 27(4), 110-122, October 2025.

First record of Brachymeria excarinata Gahan, 1925 (Hymenoptera: Chalcididae) parasitizing Plutella xylostella (L.) (Lepidoptera: Plutellidae) in west Africa

Babacar Labou, Etienne Tendeng, El hadji Sérigne Sylla, Mamadou Diatte, Karamoko Diarra, Int. J. Biosci. 27(4), 104-109, October 2025.

Assessment of adsorption isotherms of three plantain flours (Musa paradisiaca L. var. Horn 1, FHIA 21 and PITA 3) and cassava flour (Manihot esculenta Crantz var. Bonoua 2)

Brou Koffi Siméon, Yue Bi Yao Clément, Kane Fako, Douali Gohi Bi Douali Jean-Sory, Tano Kablan, Int. J. Biosci. 27(4), 93-103, October 2025.

Exploring the antioxidant efficacy of boldine: A natural compound with broad-spectrum activity

Maharani Jaganathan, Kathiresan Suresh, Manickam John, Rajeswari Vasu, Theerthu Azhamuthu, Nihal Ahamed Abulkalam Asath, Ravichandran Pugazhendhi, Pratheeba Veerapandiyan, Int. J. Biosci. 27(4), 82-92, October 2025.

Assessment of genetic parameters and yield trait stability in sweet sorghum genotypes through AMMI and GGE biplot approaches

A. H. Inuwa, H. A. Ajeigbe, Y. Mustapha, B. S. Aliyu, I. I. Angarawai, Int. J. Biosci. 27(4), 69-81, October 2025.