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Antioxidant, antibacterial activities and phenolic content of organic fractions obtained from Chamaerops humilis L. leaf and fruit

Research Paper | July 1, 2017

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Saber Belhaoues, Sandra Amri, Mourad Bensouilah, Ratiba Seridi

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Int. J. Biosci.11( 1), 284-297, July 2017

DOI: http://dx.doi.org/10.12692/ijb/11.1.284-297


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The use of plant extracts are the subject of numerous studies because, in addition to their use as preservatives in foodstuffs by replacing synthetic antioxidants, they are involved in the treatment of many diseases. This study investigated the antioxidant and antibacterial activities of Chamaerops humilis L. (Asteraceae) leaf and fruit, collected in north east of Algeria and their respective fractions, obtained using dichloromethane (DCMF), ethyl acetate (EAF), n-butanol (BF) and water (WF). The total phenolic and total flavonoids content was determined by the Folin-Ciocalteu and the aluminum chloride (2%) reaction, respectively. Antioxidant activity was measured, using different antioxidant assays (DPPH, FRAP, BCB). The antibacterial activities of the fractions were tested on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Enterococcus faecalis and Salmonella typhimurium. Two different methods were employed for the determination of antibacterial activities: disc diffusion method and minimum inhibitory concentrations (MICs). A significant correlation existed between concentrations of the extract and antioxidant activity since the maximum antioxidative activities were exhibited by ethyl acetate fraction [IC50=0.12 mg/ml (DPPH assay)] which has the highest levels of phenolic compounds (214,8±3,26 mg GAE/g fractions).Concerning antibacterial activity , the lowest activity was founded on the (WF)from fruits against Salmonella typhimurium (MIC=70mg/ml)and the highest was found on the (EA) fraction from leaves against Enterococcus faecalis (MIC=0,25 mg/ml) . These findings demonstrate the remarkable potential of this plant as new source of antioxidant ingredient with interesting antibacterial capacities.


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Antioxidant, antibacterial activities and phenolic content of organic fractions obtained from Chamaerops humilis L. leaf and fruit

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