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Assessment of selected culture media for competent growth characteristics of Ceratocystis manginecans (Cause of Mango Sudden Death)

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Research Paper 01/05/2017
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Assessment of selected culture media for competent growth characteristics of Ceratocystis manginecans (Cause of Mango Sudden Death)

Kokab Jabeen, Shahzad Asad
J. Bio. Env. Sci.10( 5), 148-154, May 2017.
Certificate: JBES 2017 [Generate Certificate]
Key: Ceratoc ystismanginecansCulture mediaMango sudden death (MSD)

Abstract

Comprehensive and critical facts of aspects which influence patterns of growth and nutrition of the fungi is precondition for research related to the relationship of host and pathogen. A number of media compositions likewise influence the colony morphology of fungus. Most conjoint and distressing disease in mango trees is Mango sudden death (MSD) caused by Ceratocystis manginecans. Present study is aimed to evaluate the influence of selected six different culture media (agar and broth media) on sporulation; colony characteristics and growth of the C. manginecans isolated from MSD infected mango trees. Statistical analysis of the data gathered revealed that all the treatments were significantly different from each other. Among solid media, Malt Extract Agar and Carrot Juice Agar supported best growth of C. manginecans89.53mm and 85.31mm respectively. Among broth media Carrot Juice supported better growth (503.33mg dry weight) than other media. The Characteristics of growth like color of substrate, colony color and margins, mycelia topography as well as sporulation of the C. manginecans were studied. Best sporulation of C. manginecans was shown in Malt Extract Agar media. Selection of most effective culture media for efficient growth characteristics of C. manginecans is necessary for its physiological and taxonomical study as well as for suitable disease management strategies.

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