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Combined effect of salinity and copper on the rates of polyphénols, flavonoids, and proline accumulation in the leaves of the Atriplex canescens (Pursh ) Nutt

Research Paper | December 1, 2017

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Ghamnia Youcef, Reguieg Yssaad Houcine Abdelhakim, Belkhoudja Moulay

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Int. J. Biosci.11( 6), 130-138, December 2017

DOI: http://dx.doi.org/10.12692/ijb/11.6.130-138


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Atriplex is a halophyte plant that accumulates high levels of salt in these tissues. It is also considered as a accumulative plant of heavy metals in the soil. Copper is considered to be a toxic metal when it is at high concentrations. This study investigated the effect of different concentrations of copper (0, 2000, 2500, and 3000 ppm) combined with 0.5 and 3% salt (NaCl) during one month of stress on the rates of polyphenols, flavonoids, and proline in the leaves of the Atriplex canescens Pursh Nutt. The analysis of the antioxidants at the leaf level of the Atriplex canescens is marked by an increase in the rates of polyphenols and flavonoids under the action of copper combined with 0.5 and 3% NaCl. The higher levels of polyphenols and flavonoids (178 mg. g-1 dry weight and 173 mg. g-1 dry weights respectively) are obtained at the dose of 3000 ppm copper combined with 3% NaCl. Plants exposed to different concentrations of copper combined with 0.5% NaCl accumulated significant amounts of proline in their leaves compared to controls, the highest levels of which are recorded in plant leaves which receive 2500 and 3000 ppm copper combined with 3% NaCl (447.8 and 546 µg .g-1 dry weight respectively)  . Finally, it is noted that the Atriplex canescens has mechanisms that allow it to tolerate excess copper combined with NaCl.


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Combined effect of salinity and copper on the rates of polyphénols, flavonoids, and proline accumulation in the leaves of the Atriplex canescens (Pursh ) Nutt

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