Direct embryogenesis of Valerian (Valeriana officinalis L.) using leaf segments

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Research Paper 01/12/2016
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Direct embryogenesis of Valerian (Valeriana officinalis L.) using leaf segments

Arsalan Shekarchian, Ghollam Hossein Rahmani, Mohammad Reza Kudori, Davod Darvishi Zeidabadi
J. Biodiv. & Environ. Sci. 9(6), 113-120, December 2016.
Copyright Statement: Copyright 2016; The Author(s).
License: CC BY-NC 4.0

Abstract

A simple procedure for direct embryogenesis without an intervening callus production was developed in valerian (Valeriana officinalis L.), using the leaf segments. Direct somatic embryogenesis was induced using half-strength Murashige and Skoog (MS) medium supplemented with 2, 4 dichlorophenoxyacetic acid (0.5mg L-1), glutamic acid (100mg L-1), 4% sucrose and 8g L-1 agar. Embryo germination to form plantlets enhanced on MS medium supplemented with naphthalene acetic acid (0.1mg L-1) and kinetin (2mg L-1). Regenerated plants with well-developed root and shoot systems were successfully transferred to greenhouse.

Bohinski RC. 1991. Metabolismo de los aminoácidos y nucleótidos. Bioquímica. Editorial Addison-Wesley Iberoamericana, 5ta edición, 737pp.

Bos R, Hendriks H, Scheffer JJC, Woerdenbag HJ, 1998. Cytotoxic potential ofvalerian constituents and valerian tinctures. Phytomedicine 5, 219-225.

Castillo P, Márquez J, Rubluo A, Hernández G, Lara M. 2000. Plant regeneration from callus and suspension cultures of Valeriana edulis ssp. procera via simultaneous organogenesis and somatic embryogenesis. Plant science 151(2), 115-119.

Chen R, Zhang M, Lü J, Zhang X, da Silva JA, Ma G. 2014. Shoot organogenesis and somatic embryogenesis from leaf explants of Valeriana jatamansi Jones. Scientia Horticulturae 22, 392-7.

Cruz GS, Canhoto JM, Abreu MAV. 1990. Somatic embryogenesis and plant regeneration from zygotic embryos of Feijoa sellowiana Berg. Plant Science 66(2), 263-270.

Deng MD, Cornu D. 1992. Maturation and germination of walnut somatic embryos. Plant cell, tissue and organ culture 28(2), 195-202.

Encisco-Rodriguez R. 1997. Micropropagation of Valeriana edulis ssp. Procera. Planta Medica 63, 274-275.

Hiller KO, Zetler G. 1996. Neuropharmacological studies on ethanol extracts of Valeriana officinalis L.: behavioural and anticonvulsant properties. Phytotherapy research 10(2), 145-151.

Ivanova A, Velcheva M, Denchev P, Atanassov A, Onckelen HA. 1994. Endogenous hormone levels during direct somatic embryogenesis in Medicago falcata. Physiologia Plantarum 92(1), 85-89.

Kaur R, Sood M., Chander S, Mahajan R, Kumar V, Sharma DR. 1999. In vitro propagation of Valeriana jatamansi. Plant cell, tissue and organ culture 59(3), 227-229.

Lea PJ, Leegood RC. 1993. Plant biochemistry and molecular biology. John Wiley & Sons. New York pp. 155-180.

Mathur J, Ahuja PS, Mathur A, Kukreja AK, Shah NC. 1988. In vitro propagation of Valeriana wallichii. Planta medica 54(01), 82-83.

Mathur J, Ahuja PS. 1991. Plant regeneration from callus cultures of Valeriana wallichii DC. Plant cell reports 9(9), 523-526.

Mathur J. 1992. Plantlet regeneration from suspension cultures of Valeriana wallichii DC. Plant Science 81(1), 111-115.

Murashige T, Skoog F. 1962. A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures. PhysiologiaPlantamm 15, 473-497.

O’Hara M, Kiefer D, Farrell K, Kemper K. 1998. A review of 12 commonly used medicinal herbs. Archives of Family Medicine 7(6), 523.

Parrott WA, Dryden G, Vogt S, Hildebrand DF, Collins GB, Williams EG. 1988. Optimization of somatic embryogenesis and embryo germination in soybean. In vitro cellular & developmental biology 24(8), 817-820.

Pedroso MC, Pais MS. 1995. Factors controlling somatic embryogenesis. Plant cell, tissue and organ culture 43(2), 147-154.

Tripathi L, Tripathi JN. 2003. Role of biotechnology in medicinal plants. Tropical Journal of Pharmaceutical Research 2(2), 243-253.

Young G, Jack D, Smith D, Saier M. 1999. The amino acid/auxin: proton symport permease family. Biochimica et Biophysica Acta (BBA)-Biomembranes 1415(2), 306-322.

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